MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2346 576.2618 1150.5090
2347 576.2618 1150.5090
2348 576.2618 1150.5090
2349 576.2618 1150.5090
2351 576.2618 1150.5090
2352 576.2618 1150.5090
2353 576.2618 1150.5090
2354 576.2618 1150.5090
2355 576.2618 1150.5090
2356 576.2618 1150.5090
2357 576.2618 1150.5090
2358 576.2618 1150.5090
2359 576.2618 1150.5090
2360 576.2618 1150.5090
2361 576.2618 1150.5090
2362 576.2618 1150.5090
2363 576.2618 1150.5090
2364 576.2618 1150.5090
2366 576.2618 1150.5090
2367 576.2618 1150.5090
2369 576.2618 1150.5090
2372 576.2618 1150.5090
2373 576.2618 1150.5090
2375 576.2618 1150.5090
2376 576.2618 1150.5090
2377 576.2618 1150.5090
2379 576.2618 1150.5090
2380 576.2618 1150.5090
2381 576.2618 1150.5090
2382 576.2618 1150.5090
2383 576.2618 1150.5090
2385 576.2618 1150.5090
2386 576.2618 1150.5090
2387 576.2618 1150.5090
2388 576.2618 1150.5090
2390 576.2618 1150.5090
2391 576.2618 1150.5090
2392 576.2618 1150.5090
2393 576.2618 1150.5090
2394 576.2618 1150.5090
2396 576.2618 1150.5090
2397 576.2618 1150.5090
2398 576.2618 1150.5090
2399 576.2618 1150.5090
2400 576.2618 1150.5090
2401 576.2618 1150.5090
2402 576.2618 1150.5090
2404 576.2618 1150.5090
2405 576.2618 1150.5090
2406 576.2618 1150.5090
2408 576.2618 1150.5090
2409 576.2618 1150.5090
2411 576.2618 1150.5090
2413 576.2618 1150.5090
2414 576.2618 1150.5090
2415 576.2618 1150.5090
2416 576.2618 1150.5090
2417 576.2618 1150.5090
2418 576.2618 1150.5090
2419 576.2618 1150.5090
2420 576.2618 1150.5090
2421 576.2618 1150.5090
2422 576.2618 1150.5090
2424 576.2618 1150.5090
2425 576.2618 1150.5090
2426 576.2618 1150.5090
2427 576.2618 1150.5090
2428 576.2618 1150.5090
2429 576.2709 1150.5272
2430 576.2719 1150.5292
2431 589.7679 1177.5212
2432 589.7679 1177.5212
2433 589.7679 1177.5212
2434 589.7679 1177.5212
2435 589.7679 1177.5212
2436 589.7679 1177.5212
2437 589.7679 1177.5212
2438 589.7679 1177.5212
2439 589.7679 1177.5212
2440 589.7679 1177.5212
2441 589.7679 1177.5212
2442 589.7679 1177.5212
2443 589.7679 1177.5212
2444 589.7679 1177.5212
2445 589.7679 1177.5212
2446 589.7679 1177.5212
2447 589.7679 1177.5212
2448 589.7679 1177.5212
2449 589.7679 1177.5212
2450 589.7679 1177.5212
2451 589.7679 1177.5212
2452 589.7679 1177.5212
2453 589.7679 1177.5212
2455 589.7679 1177.5212
2456 589.7679 1177.5212
2458 589.7679 1177.5212
2459 589.7679 1177.5212
2460 589.7679 1177.5212
2462 589.7679 1177.5212
2463 589.7679 1177.5212
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