MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | PRT1377_JBEI_2 |
| MS data file | : | PRT1377_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 23 Jan 2026 at 21:31:54 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,287 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 6842 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 6420 |
913.9153 | 1825.8160 | 1825.8304 | -7.88 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
QMYGLNAIEHSNYLR + 2 Deamidated (NQ); Oxidation (M) |
| 6702 |
940.9276 | 1879.8406 | 1879.8291 | 6.13 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
MIQEQTMLNVADNSGAR + 3 Deamidated (NQ) |
| 5037 |
805.8662 | 1609.7178 | 1609.7406 | -14.1 | 0 | 4 | 0.71 | 1Score > 15 indicates identity |
MLQESVDALFDNGR + Oxidation (M) |
| 5651 |
846.3846 | 1690.7546 | 1690.7484 | 3.72 | 0 | 4 | 0.83 | 1Score > 16 indicates identity |
QYLMQFCPDAVFR + Deamidated (NQ); Oxidation (M) |
| 6317 |
900.4092 | 1798.8038 | 1798.8196 | -8.76 | 0 | 4 | 0.69 | 1Score > 15 indicates identity |
TEGATVFGYQVMDPER |
| 6892 |
954.4337 | 1906.8528 | 1906.8805 | -14.5 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
FAMGELPDEVLEICQR + Deamidated (NQ) |
| 5257 |
819.3723 | 1636.7300 | 1636.7119 | 11.1 | 1 | 4 | 0.97 | 1Score > 16 indicates identity |
RMGELMAESHASMR + 2 Oxidation (M) |
| 5583 |
846.3846 | 1690.7546 | 1690.7403 | 8.49 | 0 | 4 | 0.83 | 1Score > 16 indicates identity |
SEMGDLAQSVSHMQR + Oxidation (M) |
| 2158 |
562.7557 | 1123.4968 | 1123.4894 | 6.65 | 0 | 4 | 0.62 | 1Score > 14 indicates identity |
NLNDTNYNR + Deamidated (NQ) |
| 2105 |
549.2505 | 1096.4864 | 1096.4893 | -2.56 | 0 | 4 | 0.68 | 1Score > 15 indicates identity |
DEMIMSAIR + 2 Oxidation (M) |
| 2537 |
589.7679 | 1177.5212 | 1177.5107 | 8.95 | 0 | 4 | 0.58 | 1Score > 14 indicates identity |
MQQSIDPAMR + 2 Deamidated (NQ) |
| 2808 |
616.7802 | 1231.5458 | 1231.5503 | -3.62 | 0 | 4 | 0.7 | 1Score > 15 indicates identity |
MTDLHTDVER + Oxidation (M) |
| 568 |
427.6943 | 853.3740 | 853.3786 | -5.34 | 0 | 4 | 0.4 | 1Score > 13 indicates identity |
NGTMMIR + 2 Oxidation (M) |
| 4275 |
738.3355 | 1474.6564 | 1474.6582 | -1.22 | 1 | 4 | 0.78 | 1Score > 15 indicates identity |
AECQNQQGNLRR + 2 Deamidated (NQ) |
| 5400 |
832.8785 | 1663.7424 | 1663.7723 | -17.9 | 1 | 4 | 0.84 | 1Score > 16 indicates identity |
TNGLMAQAQQQLGKR + 5 Deamidated (NQ); Oxidation (M) |
| 4316 |
738.3355 | 1474.6564 | 1474.6582 | -1.22 | 1 | 4 | 0.79 | 1Score > 15 indicates identity |
AECQNQQGNLRR + 2 Deamidated (NQ) |
| 3113 |
643.7925 | 1285.5704 | 1285.5761 | -4.41 | 0 | 4 | 0.67 | 1Score > 15 indicates identity |
MQAYFDQLDR |
| 5930 |
873.3969 | 1744.7792 | 1744.7735 | 3.28 | 0 | 4 | 1.4 | 1Score > 18 indicates identity |
GTLFPMCGMNLAFDR + Oxidation (M) |
| 6401 |
913.9153 | 1825.8160 | 1825.8482 | -17.6 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
EDAQLLYGFNNKQER + 2 Deamidated (NQ) |
| 4949 |
792.3600 | 1582.7054 | 1582.7045 | 0.58 | 1 | 4 | 0.67 | 1Score > 15 indicates identity |
CQYETLVENNRR + 2 Deamidated (NQ) |
| 3078 |
630.2864 | 1258.5582 | 1258.5724 | -11.3 | 1 | 4 | 0.55 | 1Score > 14 indicates identity |
VNDRSHQMEK + Oxidation (M) |
| 5797 |
859.8907 | 1717.7668 | 1717.7808 | -8.14 | 1 | 4 | 0.76 | 1Score > 15 indicates identity |
GHSFEFRINGEDPGR + Deamidated (NQ) |
| 2570 |
589.7679 | 1177.5212 | 1177.5107 | 8.95 | 0 | 4 | 0.6 | 1Score > 14 indicates identity |
MQQSIDPAMR + 2 Deamidated (NQ) |
| 3303 |
657.2986 | 1312.5826 | 1312.5757 | 5.26 | 0 | 4 | 0.91 | 1Score > 16 indicates identity |
EYTWANLMER + Deamidated (NQ) |
| 2617 |
603.2741 | 1204.5336 | 1204.5506 | -14.1 | 0 | 4 | 0.91 | 1Score > 16 indicates identity |
GCGITVDQAER |
| 4264 |
738.3355 | 1474.6564 | 1474.6324 | 16.3 | 0 | 4 | 0.81 | 1Score > 15 indicates identity |
QHQQFADQESVR + 3 Deamidated (NQ) |
| 7021 |
967.9399 | 1933.8652 | 1933.8993 | -17.6 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
LSGFDMGYSGIQFGGDKR |
| 6987 |
967.9399 | 1933.8652 | 1933.8298 | 18.3 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M) |
| 2985 |
630.2846 | 1258.5546 | 1258.5621 | -5.89 | 0 | 4 | 0.44 | 1Score > 13 indicates identity |
ISMMPGMPSHR + Oxidation (M) |
| 3934 |
711.3232 | 1420.6318 | 1420.6551 | -16.3 | 1 | 4 | 1 | 1Score > 16 indicates identity |
MRACINNQQIR + 2 Deamidated (NQ); Oxidation (M) |
| 4822 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.8 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 1705 |
522.2372 | 1042.4598 | 1042.4576 | 2.17 | 0 | 4 | 0.42 | 1Score > 13 indicates identity |
GFADAIMMR + 2 Oxidation (M) |
| 4756 |
778.8539 | 1555.6932 | 1555.7235 | -19.5 | 1 | 4 | 0.81 | 1Score > 15 indicates identity |
CAAGTGRFLDVMSR + Oxidation (M) |
| 2403 |
576.2618 | 1150.5090 | 1150.5176 | -7.40 | 0 | 4 | 0.79 | 1Score > 15 indicates identity |
ELTENEMIR + Deamidated (NQ); Oxidation (M) |
| 2788 |
616.7802 | 1231.5458 | 1231.5429 | 2.42 | 1 | 4 | 0.75 | 1Score > 15 indicates identity |
REEEEQQQR + Deamidated (NQ) |
| 2786 |
616.7802 | 1231.5458 | 1231.5656 | -16.0 | 0 | 4 | 0.75 | 1Score > 15 indicates identity |
ICEPFGPEQR |
| 4781 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.82 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 3554 |
670.8048 | 1339.5950 | 1339.6125 | -13.0 | 1 | 4 | 1 | 1Score > 16 indicates identity |
AANQAFMMERR + Oxidation (M) |
| 4841 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.83 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 3332 |
657.2986 | 1312.5826 | 1312.6057 | -17.5 | 0 | 4 | 0.96 | 1Score > 16 indicates identity |
LMITGFCWGGR + Oxidation (M) |
| 1852 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 0 | 4 | 0.44 | 1Score > 13 indicates identity |
YCVNSASLR + Deamidated (NQ) |
| 659 |
441.1930 | 880.3714 | 880.3562 | 17.3 | 0 | 4 | 0.53 | 1Score > 13 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 5246 |
819.3723 | 1636.7300 | 1636.7119 | 11.1 | 1 | 4 | 1.1 | 1Score > 16 indicates identity |
RMGELMAESHASMR + 2 Oxidation (M) |
| 2077 |
549.2496 | 1096.4846 | 1096.4924 | -7.07 | 0 | 4 | 0.75 | 1Score > 15 indicates identity |
EITEQYEGK + Deamidated (NQ) |
| 4828 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.84 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 5584 |
846.3846 | 1690.7546 | 1690.7403 | 8.49 | 0 | 4 | 0.94 | 1Score > 16 indicates identity |
SEMGDLAQSVSHMQR + Oxidation (M) |
| 5910 |
873.3969 | 1744.7792 | 1744.7904 | -6.39 | 0 | 4 | 1.6 | 1Score > 18 indicates identity |
DIDVDYASHSPQIER + Deamidated (NQ) |
| 2378 |
576.2618 | 1150.5090 | 1150.5111 | -1.76 | 0 | 3 | 0.83 | 1Score > 15 indicates identity |
LMGMTPTGNGR + Deamidated (NQ); Oxidation (M) |
| 5615 |
846.3846 | 1690.7546 | 1690.7403 | 8.49 | 0 | 3 | 0.94 | 1Score > 16 indicates identity |
SEMGDLAQSVSHMQR + Oxidation (M) |
| 6847 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 897 |
454.7066 | 907.3986 | 907.4035 | -5.38 | 0 | 3 | 0.61 | 1Score > 14 indicates identity |
AQFEEER |
| 1805 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 0 | 3 | 0.45 | 1Score > 13 indicates identity |
YCVNSASLR + Deamidated (NQ) |
| 3118 |
643.7925 | 1285.5704 | 1285.5761 | -4.41 | 0 | 3 | 0.75 | 1Score > 15 indicates identity |
MQAYFDQLDR |
| 794 |
441.2004 | 880.3862 | 880.3927 | -7.31 | 0 | 3 | 0.73 | 1Score > 15 indicates identity |
DGDGFGVSK |
| 3368 |
657.2986 | 1312.5826 | 1312.5870 | -3.33 | 0 | 3 | 1 | 1Score > 16 indicates identity |
SYITGEFGHMR + Oxidation (M) |
| 4274 |
738.3355 | 1474.6564 | 1474.6582 | -1.22 | 1 | 3 | 0.89 | 1Score > 15 indicates identity |
AECQNQQGNLRR + 2 Deamidated (NQ) |
| 667 |
441.1932 | 880.3718 | 880.3562 | 17.7 | 0 | 3 | 0.55 | 1Score > 13 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 6549 |
927.4214 | 1852.8282 | 1852.8625 | -18.5 | 1 | 3 | 1.2 | 1Score > 17 indicates identity |
LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M) |
| 155 |
387.1813 | 772.3480 | 772.3351 | 16.7 | 0 | 3 | 0.46 | 1Score > 13 indicates identity |
DPNGDVR + Deamidated (NQ) |
| 3990 |
711.3232 | 1420.6318 | 1420.6187 | 9.25 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M) |
| 6202 |
900.4092 | 1798.8038 | 1798.7953 | 4.76 | 0 | 3 | 0.8 | 1Score > 15 indicates identity |
CLNHMFAAEIYMQR + Oxidation (M) |
| 3969 |
711.3232 | 1420.6318 | 1420.6187 | 9.25 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M) |
| 654 |
441.1926 | 880.3706 | 880.3562 | 16.4 | 0 | 3 | 0.6 | 1Score > 14 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 2905 |
616.7802 | 1231.5458 | 1231.5615 | -12.7 | 1 | 3 | 0.81 | 1Score > 15 indicates identity |
IREMSDNDPR |
| 662 |
441.1931 | 880.3716 | 880.3562 | 17.5 | 0 | 3 | 0.56 | 1Score > 13 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 5599 |
846.3846 | 1690.7546 | 1690.7403 | 8.49 | 0 | 3 | 0.98 | 1Score > 16 indicates identity |
SEMGDLAQSVSHMQR + Oxidation (M) |
| 6296 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.82 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 3027 |
630.2864 | 1258.5582 | 1258.5387 | 15.5 | 0 | 3 | 0.64 | 1Score > 14 indicates identity |
NPDMPADELEK + Deamidated (NQ) |
| 1627 |
522.2314 | 1042.4482 | 1042.4277 | 19.7 | 0 | 3 | 0.52 | 1Score > 13 indicates identity |
FSLGNMDDK + Deamidated (NQ); Oxidation (M) |
| 4308 |
738.3355 | 1474.6564 | 1474.6582 | -1.22 | 1 | 3 | 0.92 | 1Score > 15 indicates identity |
AECQNQQGNLRR + 2 Deamidated (NQ) |
| 6460 |
913.9153 | 1825.8160 | 1825.8251 | -4.96 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
MTNNDTTLQLSSVLNR + 4 Deamidated (NQ); Oxidation (M) |
| 2308 |
576.2618 | 1150.5090 | 1150.5117 | -2.32 | 0 | 3 | 0.88 | 1Score > 15 indicates identity |
YMTWDNPPK |
| 2734 |
603.2741 | 1204.5336 | 1204.5394 | -4.75 | 1 | 3 | 1 | 1Score > 16 indicates identity |
EAAASEKDPCK |
| 962 |
454.7066 | 907.3986 | 907.4035 | -5.38 | 0 | 3 | 0.64 | 1Score > 14 indicates identity |
AQFEEER |
| 4050 |
711.3232 | 1420.6318 | 1420.6187 | 9.25 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M) |
| 4989 |
792.3600 | 1582.7054 | 1582.6875 | 11.4 | 0 | 3 | 0.79 | 1Score > 15 indicates identity |
VYFCDPQSPWQR + Deamidated (NQ) |
| 5592 |
846.3846 | 1690.7546 | 1690.7403 | 8.49 | 0 | 3 | 1 | 1Score > 16 indicates identity |
SEMGDLAQSVSHMQR + Oxidation (M) |
| 6314 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.83 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 4776 |
778.8539 | 1555.6932 | 1555.7123 | -12.2 | 1 | 3 | 0.91 | 1Score > 15 indicates identity |
FVENKSQLMGACR + Deamidated (NQ); Oxidation (M) |
| 2260 |
562.7557 | 1123.4968 | 1123.4894 | 6.65 | 0 | 3 | 0.75 | 1Score > 14 indicates identity |
NLNDTNYNR + Deamidated (NQ) |
| 6085 |
886.9030 | 1771.7914 | 1771.7584 | 18.7 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
DPASDQMQHWKEQR + Deamidated (NQ); Oxidation (M) |
| 954 |
454.7066 | 907.3986 | 907.4035 | -5.38 | 0 | 3 | 0.65 | 1Score > 14 indicates identity |
AQFEEER |
| 3633 |
684.3109 | 1366.6072 | 1366.6153 | -5.91 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
FQQQKDWETR + 2 Deamidated (NQ) |
| 6568 |
927.4214 | 1852.8282 | 1852.8625 | -18.5 | 1 | 3 | 1.2 | 1Score > 17 indicates identity |
LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M) |
| 6979 |
967.9399 | 1933.8652 | 1933.8476 | 9.14 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
MSTQYETQGYTINNAGR + Deamidated (NQ) |
| 7107 |
967.9399 | 1933.8652 | 1933.9017 | -18.9 | 1 | 3 | 1.3 | 1Score > 17 indicates identity |
QDIDRWQSTSVNDVLR + 3 Deamidated (NQ) |
| 1909 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 1 | 3 | 0.49 | 1Score > 13 indicates identity |
EAMGFSEKR + Oxidation (M) |
| 5775 |
859.8907 | 1717.7668 | 1717.7763 | -5.51 | 0 | 3 | 0.9 | 1Score > 15 indicates identity |
EMLEHMASTLAQGER + Oxidation (M) |
| 5399 |
832.8785 | 1663.7424 | 1663.7406 | 1.10 | 1 | 3 | 1 | 1Score > 16 indicates identity |
MDPSLNTMANDRQR + Oxidation (M) |
| 1180 |
481.7188 | 961.4230 | 961.4253 | -2.38 | 0 | 3 | 0.79 | 1Score > 15 indicates identity |
SGNDLHYR + Deamidated (NQ) |
| 1291 |
481.7188 | 961.4230 | 961.4215 | 1.59 | 0 | 3 | 0.79 | 1Score > 15 indicates identity |
GSMTFFEK + Oxidation (M) |
| 3721 |
684.3109 | 1366.6072 | 1366.6266 | -14.1 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
DWRETNIDYR |
| 6283 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.87 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 6299 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.87 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 5923 |
873.3969 | 1744.7792 | 1744.7735 | 3.28 | 0 | 3 | 1.7 | 1Score > 18 indicates identity |
GTLFPMCGMNLAFDR + Oxidation (M) |
| 6224 |
900.4092 | 1798.8038 | 1798.7953 | 4.76 | 0 | 3 | 0.87 | 1Score > 15 indicates identity |
CLNHMFAAEIYMQR + Oxidation (M) |
| 3722 |
684.3109 | 1366.6072 | 1366.6266 | -14.1 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
DWRETNIDYR |
| 6252 |
900.4092 | 1798.8038 | 1798.8308 | -15.0 | 0 | 3 | 0.88 | 1Score > 15 indicates identity |
IYDSDSILSMHHEPR |
| 6289 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.88 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
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