MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2236 562.7557 1123.4968
2237 562.7557 1123.4968
2238 562.7557 1123.4968
2239 562.7557 1123.4968
2240 562.7557 1123.4968
2241 562.7557 1123.4968
2243 562.7557 1123.4968
2244 562.7557 1123.4968
2245 562.7557 1123.4968
2246 562.7557 1123.4968
2247 562.7557 1123.4968
2248 562.7557 1123.4968
2249 562.7557 1123.4968
2250 562.7557 1123.4968
2251 562.7557 1123.4968
2252 562.7557 1123.4968
2253 562.7557 1123.4968
2254 562.7557 1123.4968
2256 562.7557 1123.4968
2257 562.7557 1123.4968
2258 562.7557 1123.4968
2259 562.7557 1123.4968
2261 562.7557 1123.4968
2262 562.7557 1123.4968
2263 562.7557 1123.4968
2264 562.7557 1123.4968
2265 562.7557 1123.4968
2266 562.7617 1123.5088
2267 562.7621 1123.5096
2268 562.7628 1123.5110
2269 576.2512 1150.4878
2270 576.2557 1150.4968
2271 576.2565 1150.4984
2272 576.2587 1150.5028
2273 576.2618 1150.5090
2274 576.2618 1150.5090
2275 576.2618 1150.5090
2276 576.2618 1150.5090
2277 576.2618 1150.5090
2278 576.2618 1150.5090
2279 576.2618 1150.5090
2280 576.2618 1150.5090
2281 576.2618 1150.5090
2282 576.2618 1150.5090
2283 576.2618 1150.5090
2284 576.2618 1150.5090
2285 576.2618 1150.5090
2286 576.2618 1150.5090
2287 576.2618 1150.5090
2288 576.2618 1150.5090
2289 576.2618 1150.5090
2290 576.2618 1150.5090
2291 576.2618 1150.5090
2292 576.2618 1150.5090
2293 576.2618 1150.5090
2294 576.2618 1150.5090
2295 576.2618 1150.5090
2297 576.2618 1150.5090
2298 576.2618 1150.5090
2299 576.2618 1150.5090
2300 576.2618 1150.5090
2301 576.2618 1150.5090
2302 576.2618 1150.5090
2303 576.2618 1150.5090
2304 576.2618 1150.5090
2305 576.2618 1150.5090
2306 576.2618 1150.5090
2307 576.2618 1150.5090
2309 576.2618 1150.5090
2310 576.2618 1150.5090
2311 576.2618 1150.5090
2313 576.2618 1150.5090
2314 576.2618 1150.5090
2315 576.2618 1150.5090
2316 576.2618 1150.5090
2317 576.2618 1150.5090
2319 576.2618 1150.5090
2320 576.2618 1150.5090
2321 576.2618 1150.5090
2322 576.2618 1150.5090
2323 576.2618 1150.5090
2324 576.2618 1150.5090
2325 576.2618 1150.5090
2326 576.2618 1150.5090
2327 576.2618 1150.5090
2328 576.2618 1150.5090
2329 576.2618 1150.5090
2330 576.2618 1150.5090
2331 576.2618 1150.5090
2332 576.2618 1150.5090
2333 576.2618 1150.5090
2334 576.2618 1150.5090
2335 576.2618 1150.5090
2338 576.2618 1150.5090
2339 576.2618 1150.5090
2340 576.2618 1150.5090
2341 576.2618 1150.5090
2342 576.2618 1150.5090
2343 576.2618 1150.5090
2345 576.2618 1150.5090
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