MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1979 549.2496 1096.4846
1980 549.2496 1096.4846
1981 549.2496 1096.4846
1982 549.2496 1096.4846
1983 549.2496 1096.4846
1984 549.2496 1096.4846
1985 549.2496 1096.4846
1986 549.2496 1096.4846
1988 549.2496 1096.4846
1989 549.2496 1096.4846
1991 549.2496 1096.4846
1992 549.2496 1096.4846
1993 549.2496 1096.4846
1994 549.2496 1096.4846
1995 549.2496 1096.4846
1996 549.2496 1096.4846
1997 549.2496 1096.4846
1998 549.2496 1096.4846
1999 549.2496 1096.4846
2000 549.2496 1096.4846
2001 549.2496 1096.4846
2002 549.2496 1096.4846
2003 549.2496 1096.4846
2004 549.2496 1096.4846
2005 549.2496 1096.4846
2006 549.2496 1096.4846
2007 549.2496 1096.4846
2008 549.2496 1096.4846
2009 549.2496 1096.4846
2010 549.2496 1096.4846
2011 549.2496 1096.4846
2012 549.2496 1096.4846
2013 549.2496 1096.4846
2014 549.2496 1096.4846
2015 549.2496 1096.4846
2016 549.2496 1096.4846
2019 549.2496 1096.4846
2022 549.2496 1096.4846
2023 549.2496 1096.4846
2024 549.2496 1096.4846
2025 549.2496 1096.4846
2028 549.2496 1096.4846
2029 549.2496 1096.4846
2030 549.2496 1096.4846
2031 549.2496 1096.4846
2032 549.2496 1096.4846
2033 549.2496 1096.4846
2037 549.2496 1096.4846
2039 549.2496 1096.4846
2040 549.2496 1096.4846
2042 549.2496 1096.4846
2043 549.2496 1096.4846
2044 549.2496 1096.4846
2048 549.2496 1096.4846
2049 549.2496 1096.4846
2050 549.2496 1096.4846
2053 549.2496 1096.4846
2056 549.2496 1096.4846
2057 549.2496 1096.4846
2058 549.2496 1096.4846
2062 549.2496 1096.4846
2063 549.2496 1096.4846
2066 549.2496 1096.4846
2067 549.2496 1096.4846
2070 549.2496 1096.4846
2073 549.2496 1096.4846
2074 549.2496 1096.4846
2076 549.2496 1096.4846
2078 549.2496 1096.4846
2080 549.2496 1096.4846
2081 549.2496 1096.4846
2083 549.2496 1096.4846
2084 549.2496 1096.4846
2085 549.2496 1096.4846
2088 549.2496 1096.4846
2089 549.2496 1096.4846
2090 549.2496 1096.4846
2091 549.2496 1096.4846
2092 549.2496 1096.4846
2094 549.2496 1096.4846
2095 549.2496 1096.4846
2096 549.2496 1096.4846
2097 549.2496 1096.4846
2098 549.2496 1096.4846
2099 549.2496 1096.4846
2100 549.2496 1096.4846
2101 549.2496 1096.4846
2102 549.2496 1096.4846
2103 549.2496 1096.4846
2107 562.7557 1123.4968
2108 562.7557 1123.4968
2109 562.7557 1123.4968
2110 562.7557 1123.4968
2111 562.7557 1123.4968
2112 562.7557 1123.4968
2113 562.7557 1123.4968
2114 562.7557 1123.4968
2115 562.7557 1123.4968
2116 562.7557 1123.4968
2117 562.7557 1123.4968
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