MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1861 535.7434 1069.4722
1862 535.7434 1069.4722
1863 535.7434 1069.4722
1864 535.7434 1069.4722
1865 535.7434 1069.4722
1866 535.7434 1069.4722
1868 535.7434 1069.4722
1869 535.7434 1069.4722
1870 535.7434 1069.4722
1871 535.7434 1069.4722
1872 535.7434 1069.4722
1873 535.7434 1069.4722
1874 535.7434 1069.4722
1875 535.7434 1069.4722
1876 535.7434 1069.4722
1877 535.7434 1069.4722
1878 535.7434 1069.4722
1879 535.7434 1069.4722
1880 535.7434 1069.4722
1881 535.7434 1069.4722
1882 535.7434 1069.4722
1884 535.7434 1069.4722
1885 535.7434 1069.4722
1886 535.7434 1069.4722
1887 535.7434 1069.4722
1888 535.7434 1069.4722
1889 535.7434 1069.4722
1890 535.7434 1069.4722
1891 535.7434 1069.4722
1892 535.7434 1069.4722
1893 535.7434 1069.4722
1894 535.7434 1069.4722
1895 535.7434 1069.4722
1896 535.7434 1069.4722
1897 535.7434 1069.4722
1898 535.7434 1069.4722
1899 535.7434 1069.4722
1900 535.7434 1069.4722
1901 535.7434 1069.4722
1903 535.7434 1069.4722
1904 535.7434 1069.4722
1905 535.7434 1069.4722
1907 535.7434 1069.4722
1908 535.7434 1069.4722
1910 535.7434 1069.4722
1911 535.7434 1069.4722
1912 535.7434 1069.4722
1913 535.7434 1069.4722
1914 535.7434 1069.4722
1915 535.7434 1069.4722
1916 535.7434 1069.4722
1917 535.7434 1069.4722
1918 535.7434 1069.4722
1919 535.7434 1069.4722
1920 535.7434 1069.4722
1921 535.7434 1069.4722
1922 535.7434 1069.4722
1923 535.7434 1069.4722
1924 535.7434 1069.4722
1925 535.7434 1069.4722
1926 535.7434 1069.4722
1927 535.7434 1069.4722
1928 535.7434 1069.4722
1929 535.7434 1069.4722
1930 535.7434 1069.4722
1931 535.7434 1069.4722
1932 535.7434 1069.4722
1933 535.7434 1069.4722
1934 535.7434 1069.4722
1936 535.7434 1069.4722
1937 535.7434 1069.4722
1938 535.7434 1069.4722
1939 535.7434 1069.4722
1940 535.7434 1069.4722
1941 535.7434 1069.4722
1942 535.7434 1069.4722
1943 535.7434 1069.4722
1944 535.7434 1069.4722
1945 549.2401 1096.4656
1946 549.2440 1096.4734
1947 549.2496 1096.4846
1948 549.2496 1096.4846
1949 549.2496 1096.4846
1950 549.2496 1096.4846
1951 549.2496 1096.4846
1952 549.2496 1096.4846
1953 549.2496 1096.4846
1955 549.2496 1096.4846
1956 549.2496 1096.4846
1957 549.2496 1096.4846
1962 549.2496 1096.4846
1963 549.2496 1096.4846
1964 549.2496 1096.4846
1965 549.2496 1096.4846
1970 549.2496 1096.4846
1971 549.2496 1096.4846
1973 549.2496 1096.4846
1975 549.2496 1096.4846
1977 549.2496 1096.4846
1978 549.2496 1096.4846
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