MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1745 522.2372 1042.4598
1746 522.2372 1042.4598
1747 522.2372 1042.4598
1748 522.2372 1042.4598
1749 522.2372 1042.4598
1750 522.2372 1042.4598
1751 522.2372 1042.4598
1752 522.2372 1042.4598
1753 522.2372 1042.4598
1754 522.2372 1042.4598
1755 522.2372 1042.4598
1756 522.2372 1042.4598
1757 522.2372 1042.4598
1758 522.2372 1042.4598
1759 522.2372 1042.4598
1760 522.2372 1042.4598
1761 522.2372 1042.4598
1762 522.2372 1042.4598
1763 522.2372 1042.4598
1764 522.2372 1042.4598
1765 522.2372 1042.4598
1766 522.2372 1042.4598
1767 522.2372 1042.4598
1768 522.2372 1042.4598
1769 522.2372 1042.4598
1770 522.2372 1042.4598
1771 522.2372 1042.4598
1772 522.2372 1042.4598
1773 522.2372 1042.4598
1774 522.2372 1042.4598
1775 522.2372 1042.4598
1776 522.2372 1042.4598
1777 522.2380 1042.4614
1778 522.2382 1042.4618
1779 522.2396 1042.4646
1780 522.2433 1042.4720
1781 522.2453 1042.4760
1782 522.2461 1042.4776
1783 535.7335 1069.4524
1784 535.7434 1069.4722
1785 535.7434 1069.4722
1786 535.7434 1069.4722
1787 535.7434 1069.4722
1788 535.7434 1069.4722
1789 535.7434 1069.4722
1790 535.7434 1069.4722
1791 535.7434 1069.4722
1792 535.7434 1069.4722
1793 535.7434 1069.4722
1794 535.7434 1069.4722
1795 535.7434 1069.4722
1796 535.7434 1069.4722
1797 535.7434 1069.4722
1798 535.7434 1069.4722
1799 535.7434 1069.4722
1800 535.7434 1069.4722
1801 535.7434 1069.4722
1802 535.7434 1069.4722
1803 535.7434 1069.4722
1804 535.7434 1069.4722
1806 535.7434 1069.4722
1807 535.7434 1069.4722
1809 535.7434 1069.4722
1812 535.7434 1069.4722
1813 535.7434 1069.4722
1814 535.7434 1069.4722
1815 535.7434 1069.4722
1816 535.7434 1069.4722
1817 535.7434 1069.4722
1818 535.7434 1069.4722
1821 535.7434 1069.4722
1822 535.7434 1069.4722
1823 535.7434 1069.4722
1824 535.7434 1069.4722
1825 535.7434 1069.4722
1826 535.7434 1069.4722
1829 535.7434 1069.4722
1830 535.7434 1069.4722
1833 535.7434 1069.4722
1834 535.7434 1069.4722
1835 535.7434 1069.4722
1836 535.7434 1069.4722
1837 535.7434 1069.4722
1841 535.7434 1069.4722
1842 535.7434 1069.4722
1843 535.7434 1069.4722
1844 535.7434 1069.4722
1845 535.7434 1069.4722
1846 535.7434 1069.4722
1847 535.7434 1069.4722
1848 535.7434 1069.4722
1849 535.7434 1069.4722
1850 535.7434 1069.4722
1854 535.7434 1069.4722
1855 535.7434 1069.4722
1856 535.7434 1069.4722
1857 535.7434 1069.4722
1858 535.7434 1069.4722
1859 535.7434 1069.4722
1860 535.7434 1069.4722
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