MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1634 522.2358 1042.4570
1635 522.2360 1042.4574
1636 522.2361 1042.4576
1637 522.2372 1042.4598
1638 522.2372 1042.4598
1639 522.2372 1042.4598
1640 522.2372 1042.4598
1641 522.2372 1042.4598
1642 522.2372 1042.4598
1643 522.2372 1042.4598
1644 522.2372 1042.4598
1645 522.2372 1042.4598
1646 522.2372 1042.4598
1647 522.2372 1042.4598
1648 522.2372 1042.4598
1649 522.2372 1042.4598
1650 522.2372 1042.4598
1651 522.2372 1042.4598
1652 522.2372 1042.4598
1653 522.2372 1042.4598
1654 522.2372 1042.4598
1655 522.2372 1042.4598
1656 522.2372 1042.4598
1657 522.2372 1042.4598
1658 522.2372 1042.4598
1659 522.2372 1042.4598
1660 522.2372 1042.4598
1661 522.2372 1042.4598
1662 522.2372 1042.4598
1663 522.2372 1042.4598
1664 522.2372 1042.4598
1665 522.2372 1042.4598
1666 522.2372 1042.4598
1667 522.2372 1042.4598
1668 522.2372 1042.4598
1669 522.2372 1042.4598
1670 522.2372 1042.4598
1671 522.2372 1042.4598
1672 522.2372 1042.4598
1673 522.2372 1042.4598
1674 522.2372 1042.4598
1675 522.2372 1042.4598
1676 522.2372 1042.4598
1677 522.2372 1042.4598
1678 522.2372 1042.4598
1679 522.2372 1042.4598
1681 522.2372 1042.4598
1682 522.2372 1042.4598
1683 522.2372 1042.4598
1684 522.2372 1042.4598
1685 522.2372 1042.4598
1689 522.2372 1042.4598
1690 522.2372 1042.4598
1691 522.2372 1042.4598
1693 522.2372 1042.4598
1694 522.2372 1042.4598
1695 522.2372 1042.4598
1696 522.2372 1042.4598
1697 522.2372 1042.4598
1698 522.2372 1042.4598
1699 522.2372 1042.4598
1700 522.2372 1042.4598
1701 522.2372 1042.4598
1702 522.2372 1042.4598
1703 522.2372 1042.4598
1706 522.2372 1042.4598
1708 522.2372 1042.4598
1709 522.2372 1042.4598
1710 522.2372 1042.4598
1711 522.2372 1042.4598
1712 522.2372 1042.4598
1713 522.2372 1042.4598
1714 522.2372 1042.4598
1715 522.2372 1042.4598
1716 522.2372 1042.4598
1717 522.2372 1042.4598
1718 522.2372 1042.4598
1719 522.2372 1042.4598
1721 522.2372 1042.4598
1723 522.2372 1042.4598
1724 522.2372 1042.4598
1725 522.2372 1042.4598
1726 522.2372 1042.4598
1727 522.2372 1042.4598
1728 522.2372 1042.4598
1729 522.2372 1042.4598
1730 522.2372 1042.4598
1731 522.2372 1042.4598
1732 522.2372 1042.4598
1733 522.2372 1042.4598
1735 522.2372 1042.4598
1736 522.2372 1042.4598
1737 522.2372 1042.4598
1738 522.2372 1042.4598
1739 522.2372 1042.4598
1740 522.2372 1042.4598
1741 522.2372 1042.4598
1742 522.2372 1042.4598
1743 522.2372 1042.4598
1744 522.2372 1042.4598
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