MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1528 508.7311 1015.4476
1529 508.7311 1015.4476
1530 508.7311 1015.4476
1531 508.7311 1015.4476
1532 508.7311 1015.4476
1533 508.7311 1015.4476
1534 508.7311 1015.4476
1535 508.7311 1015.4476
1536 508.7311 1015.4476
1537 508.7311 1015.4476
1538 508.7311 1015.4476
1539 508.7311 1015.4476
1540 508.7311 1015.4476
1541 508.7311 1015.4476
1542 508.7311 1015.4476
1543 508.7311 1015.4476
1544 508.7311 1015.4476
1545 508.7311 1015.4476
1546 508.7311 1015.4476
1547 508.7311 1015.4476
1548 508.7311 1015.4476
1549 508.7311 1015.4476
1550 508.7311 1015.4476
1551 508.7311 1015.4476
1553 508.7311 1015.4476
1554 508.7311 1015.4476
1555 508.7311 1015.4476
1556 508.7311 1015.4476
1557 508.7311 1015.4476
1558 508.7311 1015.4476
1559 508.7311 1015.4476
1560 508.7311 1015.4476
1561 508.7311 1015.4476
1562 508.7311 1015.4476
1563 508.7311 1015.4476
1564 508.7311 1015.4476
1565 508.7311 1015.4476
1566 508.7311 1015.4476
1567 508.7311 1015.4476
1568 508.7311 1015.4476
1569 508.7311 1015.4476
1570 508.7311 1015.4476
1571 508.7311 1015.4476
1572 508.7311 1015.4476
1573 508.7311 1015.4476
1574 508.7311 1015.4476
1575 508.7311 1015.4476
1576 508.7311 1015.4476
1577 508.7311 1015.4476
1578 508.7311 1015.4476
1579 508.7311 1015.4476
1580 508.7311 1015.4476
1581 508.7311 1015.4476
1582 508.7311 1015.4476
1583 508.7311 1015.4476
1585 508.7311 1015.4476
1586 508.7311 1015.4476
1587 508.7311 1015.4476
1588 508.7311 1015.4476
1589 508.7311 1015.4476
1590 508.7311 1015.4476
1591 508.7311 1015.4476
1592 508.7311 1015.4476
1593 508.7311 1015.4476
1594 508.7311 1015.4476
1595 508.7311 1015.4476
1596 508.7311 1015.4476
1597 508.7311 1015.4476
1598 508.7311 1015.4476
1599 508.7311 1015.4476
1600 508.7311 1015.4476
1601 508.7311 1015.4476
1602 508.7311 1015.4476
1603 508.7311 1015.4476
1604 508.7311 1015.4476
1605 508.7311 1015.4476
1606 508.7311 1015.4476
1607 508.7311 1015.4476
1608 508.7311 1015.4476
1609 508.7311 1015.4476
1610 508.7311 1015.4476
1611 508.7311 1015.4476
1612 508.7311 1015.4476
1613 508.7311 1015.4476
1614 508.7311 1015.4476
1615 508.7311 1015.4476
1616 508.7311 1015.4476
1617 508.7311 1015.4476
1618 508.7311 1015.4476
1619 508.7355 1015.4564
1620 508.7358 1015.4570
1622 522.2277 1042.4408
1623 522.2285 1042.4424
1626 522.2313 1042.4480
1628 522.2322 1042.4498
1629 522.2351 1042.4556
1630 522.2352 1042.4558
1631 522.2352 1042.4558
1632 522.2352 1042.4558
1633 522.2356 1042.4566
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