MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1426 495.2250 988.4354
1427 495.2250 988.4354
1428 495.2250 988.4354
1429 495.2250 988.4354
1430 495.2250 988.4354
1431 495.2250 988.4354
1432 495.2250 988.4354
1434 495.2250 988.4354
1435 495.2250 988.4354
1436 495.2250 988.4354
1437 495.2250 988.4354
1438 495.2250 988.4354
1439 495.2250 988.4354
1441 495.2250 988.4354
1442 495.2250 988.4354
1443 495.2250 988.4354
1444 495.2250 988.4354
1445 495.2250 988.4354
1446 495.2250 988.4354
1447 495.2250 988.4354
1448 495.2250 988.4354
1449 495.2250 988.4354
1450 495.2250 988.4354
1451 495.2250 988.4354
1452 495.2250 988.4354
1453 495.2250 988.4354
1454 495.2250 988.4354
1455 495.2250 988.4354
1456 495.2250 988.4354
1457 495.2250 988.4354
1458 495.2250 988.4354
1459 508.7248 1015.4350
1460 508.7260 1015.4374
1461 508.7261 1015.4376
1462 508.7262 1015.4378
1463 508.7265 1015.4384
1464 508.7275 1015.4404
1465 508.7275 1015.4404
1466 508.7275 1015.4404
1467 508.7277 1015.4408
1468 508.7280 1015.4414
1469 508.7281 1015.4416
1470 508.7289 1015.4432
1471 508.7292 1015.4438
1472 508.7293 1015.4440
1473 508.7294 1015.4442
1474 508.7294 1015.4442
1475 508.7304 1015.4462
1476 508.7305 1015.4464
1477 508.7306 1015.4466
1478 508.7307 1015.4468
1479 508.7311 1015.4476
1480 508.7311 1015.4476
1481 508.7311 1015.4476
1482 508.7311 1015.4476
1483 508.7311 1015.4476
1484 508.7311 1015.4476
1485 508.7311 1015.4476
1486 508.7311 1015.4476
1487 508.7311 1015.4476
1488 508.7311 1015.4476
1489 508.7311 1015.4476
1490 508.7311 1015.4476
1491 508.7311 1015.4476
1492 508.7311 1015.4476
1493 508.7311 1015.4476
1494 508.7311 1015.4476
1495 508.7311 1015.4476
1496 508.7311 1015.4476
1497 508.7311 1015.4476
1498 508.7311 1015.4476
1499 508.7311 1015.4476
1500 508.7311 1015.4476
1501 508.7311 1015.4476
1502 508.7311 1015.4476
1503 508.7311 1015.4476
1504 508.7311 1015.4476
1505 508.7311 1015.4476
1506 508.7311 1015.4476
1507 508.7311 1015.4476
1508 508.7311 1015.4476
1509 508.7311 1015.4476
1510 508.7311 1015.4476
1511 508.7311 1015.4476
1512 508.7311 1015.4476
1513 508.7311 1015.4476
1514 508.7311 1015.4476
1515 508.7311 1015.4476
1516 508.7311 1015.4476
1517 508.7311 1015.4476
1518 508.7311 1015.4476
1519 508.7311 1015.4476
1520 508.7311 1015.4476
1521 508.7311 1015.4476
1522 508.7311 1015.4476
1523 508.7311 1015.4476
1524 508.7311 1015.4476
1525 508.7311 1015.4476
1526 508.7311 1015.4476
1527 508.7311 1015.4476
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