MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1326 495.2220 988.4294
1327 495.2220 988.4294
1328 495.2220 988.4294
1329 495.2220 988.4294
1330 495.2220 988.4294
1331 495.2222 988.4298
1332 495.2222 988.4298
1333 495.2223 988.4300
1334 495.2223 988.4300
1335 495.2224 988.4302
1336 495.2224 988.4302
1337 495.2224 988.4302
1338 495.2224 988.4302
1339 495.2224 988.4302
1340 495.2225 988.4304
1341 495.2226 988.4306
1342 495.2226 988.4306
1343 495.2227 988.4308
1344 495.2227 988.4308
1345 495.2228 988.4310
1346 495.2228 988.4310
1347 495.2228 988.4310
1348 495.2229 988.4312
1349 495.2229 988.4312
1350 495.2230 988.4314
1351 495.2230 988.4314
1352 495.2230 988.4314
1353 495.2231 988.4316
1354 495.2231 988.4316
1355 495.2231 988.4316
1356 495.2231 988.4316
1357 495.2231 988.4316
1358 495.2232 988.4318
1359 495.2232 988.4318
1360 495.2232 988.4318
1361 495.2232 988.4318
1362 495.2234 988.4322
1363 495.2234 988.4322
1364 495.2234 988.4322
1365 495.2235 988.4324
1366 495.2235 988.4324
1367 495.2235 988.4324
1368 495.2236 988.4326
1369 495.2236 988.4326
1370 495.2236 988.4326
1371 495.2236 988.4326
1372 495.2237 988.4328
1373 495.2237 988.4328
1374 495.2238 988.4330
1375 495.2238 988.4330
1376 495.2238 988.4330
1377 495.2239 988.4332
1378 495.2239 988.4332
1379 495.2240 988.4334
1380 495.2240 988.4334
1381 495.2240 988.4334
1382 495.2241 988.4336
1383 495.2241 988.4336
1384 495.2241 988.4336
1385 495.2241 988.4336
1386 495.2242 988.4338
1387 495.2243 988.4340
1388 495.2244 988.4342
1389 495.2247 988.4348
1390 495.2250 988.4354
1391 495.2250 988.4354
1392 495.2250 988.4354
1393 495.2250 988.4354
1394 495.2250 988.4354
1395 495.2250 988.4354
1396 495.2250 988.4354
1397 495.2250 988.4354
1398 495.2250 988.4354
1399 495.2250 988.4354
1400 495.2250 988.4354
1401 495.2250 988.4354
1402 495.2250 988.4354
1403 495.2250 988.4354
1404 495.2250 988.4354
1405 495.2250 988.4354
1406 495.2250 988.4354
1407 495.2250 988.4354
1408 495.2250 988.4354
1409 495.2250 988.4354
1410 495.2250 988.4354
1411 495.2250 988.4354
1412 495.2250 988.4354
1413 495.2250 988.4354
1414 495.2250 988.4354
1415 495.2250 988.4354
1416 495.2250 988.4354
1417 495.2250 988.4354
1418 495.2250 988.4354
1419 495.2250 988.4354
1420 495.2250 988.4354
1421 495.2250 988.4354
1422 495.2250 988.4354
1423 495.2250 988.4354
1424 495.2250 988.4354
1425 495.2250 988.4354
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