MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 7204)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1219 481.7188 961.4230
1220 481.7188 961.4230
1221 481.7188 961.4230
1223 481.7188 961.4230
1224 481.7188 961.4230
1225 481.7188 961.4230
1226 481.7188 961.4230
1227 481.7188 961.4230
1228 481.7188 961.4230
1229 481.7188 961.4230
1230 481.7188 961.4230
1231 481.7188 961.4230
1232 481.7188 961.4230
1233 481.7188 961.4230
1234 481.7188 961.4230
1235 481.7188 961.4230
1236 481.7188 961.4230
1237 481.7188 961.4230
1238 481.7188 961.4230
1240 481.7188 961.4230
1241 481.7188 961.4230
1242 481.7188 961.4230
1243 481.7188 961.4230
1244 481.7188 961.4230
1245 481.7188 961.4230
1246 481.7188 961.4230
1247 481.7188 961.4230
1248 481.7188 961.4230
1251 481.7188 961.4230
1252 481.7188 961.4230
1253 481.7188 961.4230
1254 481.7188 961.4230
1255 481.7188 961.4230
1256 481.7188 961.4230
1257 481.7188 961.4230
1258 481.7188 961.4230
1259 481.7188 961.4230
1260 481.7188 961.4230
1261 481.7188 961.4230
1262 481.7188 961.4230
1263 481.7188 961.4230
1264 481.7188 961.4230
1265 481.7188 961.4230
1266 481.7188 961.4230
1267 481.7188 961.4230
1268 481.7188 961.4230
1269 481.7188 961.4230
1270 481.7188 961.4230
1271 481.7188 961.4230
1272 481.7188 961.4230
1273 481.7188 961.4230
1274 481.7188 961.4230
1275 481.7188 961.4230
1276 481.7188 961.4230
1277 481.7188 961.4230
1278 481.7188 961.4230
1280 481.7188 961.4230
1282 481.7188 961.4230
1283 481.7188 961.4230
1284 481.7188 961.4230
1285 481.7188 961.4230
1286 481.7188 961.4230
1287 481.7188 961.4230
1288 481.7188 961.4230
1289 481.7188 961.4230
1290 481.7188 961.4230
1292 481.7188 961.4230
1293 481.7265 961.4384
1294 481.7270 961.4394
1295 481.7279 961.4412
1296 481.7279 961.4412
1297 495.2160 988.4174
1298 495.2162 988.4178
1299 495.2166 988.4186
1300 495.2166 988.4186
1301 495.2179 988.4212
1302 495.2181 988.4216
1303 495.2189 988.4232
1304 495.2192 988.4238
1305 495.2193 988.4240
1306 495.2193 988.4240
1307 495.2194 988.4242
1308 495.2204 988.4262
1309 495.2205 988.4264
1310 495.2209 988.4272
1311 495.2210 988.4274
1312 495.2210 988.4274
1313 495.2211 988.4276
1314 495.2211 988.4276
1315 495.2213 988.4280
1316 495.2214 988.4282
1317 495.2215 988.4284
1318 495.2215 988.4284
1319 495.2216 988.4286
1320 495.2216 988.4286
1321 495.2219 988.4292
1322 495.2219 988.4292
1323 495.2219 988.4292
1324 495.2219 988.4292
1325 495.2220 988.4294
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