MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
789 441.2004 880.3862 880.3927 -7.31 0 7 0.35 +1Score > 15 indicates identity DGDGFGVSK
6311 900.4092 1798.8038 1798.7870 9.35 0 7 0.39 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
926 454.7066 907.3986 907.3957 3.29 0 6 0.3 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
1164 481.7188 961.4230 961.4253 -2.38 0 6 0.36 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
2880 616.7802 1231.5458 1231.5503 -3.62 0 6 0.39 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
3052 630.2864 1258.5582 1258.5830 -19.6 1 6 0.31 +1Score > 14 indicates identity QVRSFTYDDK + Deamidated (NQ)
5606 846.3846 1690.7546 1690.7832 -16.9 0 6 0.48 +1Score > 16 indicates identity NGIANNMLLENGGSLR + 3 Deamidated (NQ); Oxidation (M)
6823 954.4337 1906.8528 1906.8784 -13.4 0 6 0.67 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
6093 886.9030 1771.7914 1771.7909 0.29 0 6 0.53 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
5281 819.3723 1636.7300 1636.7403 -6.24 0 6 0.58 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
663 441.1931 880.3716 880.3709 0.87 0 6 0.29 +1Score > 13 indicates identity ATDSATCR
1203 481.7188 961.4230 961.4253 -2.38 0 6 0.38 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
1218 481.7188 961.4230 961.4253 -2.38 0 6 0.39 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
5605 846.3846 1690.7546 1690.7403 8.49 0 6 0.51 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
4314 738.3355 1474.6564 1474.6582 -1.22 1 6 0.49 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
5531 846.3846 1690.7546 1690.7430 6.91 0 6 0.53 +1Score > 16 indicates identity YSTEMMNVISAGLDK + Deamidated (NQ); 2 Oxidation (M)
872 454.7066 907.3986 907.3957 3.29 0 6 0.34 +1Score > 14 indicates identity QMQNELK + 2 Deamidated (NQ); Oxidation (M)
4493 751.8416 1501.6686 1501.6983 -19.8 1 6 0.68 +1Score > 17 indicates identity ERFLENAMYASR + Oxidation (M)
5588 846.3846 1690.7546 1690.7403 8.49 0 6 0.53 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
2733 603.2741 1204.5336 1204.5394 -4.75 1 6 0.56 +1Score > 16 indicates identity EAAASEKDPCK
4324 738.3355 1474.6564 1474.6582 -1.22 1 6 0.5 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
6831 954.4337 1906.8528 1906.8784 -13.4 0 6 0.75 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
6746 940.9276 1879.8406 1879.8258 7.90 0 6 0.78 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
3827 697.8171 1393.6196 1393.6402 -14.7 0 6 0.62 +1Score > 16 indicates identity SSINPFLFPGEGE + Deamidated (NQ)
4682 765.3478 1528.6810 1528.7005 -12.7 0 6 0.42 +1Score > 15 indicates identity EVPGVQLTNEGDNR + 2 Deamidated (NQ)
3860 697.8171 1393.6196 1393.6217 -1.50 0 6 0.63 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
2933 630.2816 1258.5486 1258.5724 -18.9 1 6 0.26 +1Score > 13 indicates identity TAMGEPGPDGRR + Oxidation (M)
3346 657.2986 1312.5826 1312.6081 -19.4 1 6 0.58 +1Score > 16 indicates identity AGGTIMFSNNKR + 2 Deamidated (NQ); Oxidation (M)
2972 630.2842 1258.5538 1258.5387 12.0 0 6 0.3 +1Score > 13 indicates identity YDSSATLDEMK
353 414.1853 826.3560 826.3565 -0.50 0 6 0.31 +1Score > 13 indicates identity MLGQMSK + Deamidated (NQ); 2 Oxidation (M)
1969 549.2496 1096.4846 1096.4924 -7.07 0 6 0.47 +1Score > 15 indicates identity EITEQYEGK + Deamidated (NQ)
6331 913.9153 1825.8160 1825.8087 4.02 1 6 0.77 +1Score > 17 indicates identity NGMECGIGVKNYNDVR + Deamidated (NQ)
760 441.2004 880.3862 880.3927 -7.31 0 6 0.44 +1Score > 15 indicates identity DGDGFGVSK
1121 468.2127 934.4108 934.4145 -3.86 0 5 0.54 +1Score > 15 indicates identity TDGTWEAR
2242 562.7557 1123.4968 1123.5114 -13.0 0 5 0.44 +1Score > 14 indicates identity ISGTGMCNIR + Oxidation (M)
6403 913.9153 1825.8160 1825.8482 -17.6 1 5 0.8 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
1976 549.2496 1096.4846 1096.4825 1.93 0 5 0.49 +1Score > 15 indicates identity YVTWEDQR + Deamidated (NQ)
6737 940.9276 1879.8406 1879.8557 -7.98 0 5 0.86 +1Score > 17 indicates identity MQEMFGEATNPTIAQGR
3631 684.3109 1366.6072 1366.6339 -19.5 1 5 0.63 +1Score > 16 indicates identity NFGMPAPEGYRK + Deamidated (NQ)
4609 765.3478 1528.6810 1528.6576 15.3 0 5 0.46 +1Score > 15 indicates identity HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M)
5283 819.3723 1636.7300 1636.7119 11.1 1 5 0.72 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
5608 846.3846 1690.7546 1690.7403 8.49 0 5 0.61 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
4322 738.3355 1474.6564 1474.6293 18.4 0 5 0.58 +1Score > 15 indicates identity NTVCTHCQAINR + 2 Deamidated (NQ)
2370 576.2618 1150.5090 1150.5288 -17.2 1 5 0.55 +1Score > 15 indicates identity DDLSGKDVMR + Oxidation (M)
5248 819.3723 1636.7300 1636.7119 11.1 1 5 0.73 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
5361 832.8785 1663.7424 1663.7406 1.10 1 5 0.64 +1Score > 16 indicates identity MDPSLNTMANDRQR + Oxidation (M)
5949 873.3969 1744.7792 1744.7735 3.28 0 5 1.1 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
3796 697.8171 1393.6196 1393.6217 -1.50 0 5 0.73 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
4403 751.8416 1501.6686 1501.6943 -17.1 1 5 0.82 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
3980 711.3232 1420.6318 1420.6187 9.25 1 5 0.75 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
6846 954.4337 1906.8528 1906.8784 -13.4 0 5 0.89 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
2930 630.2811 1258.5476 1258.5724 -19.7 1 5 0.31 +1Score > 13 indicates identity TAMGEPGPDGRR + Oxidation (M)
6344 913.9153 1825.8160 1825.8451 -15.9 1 5 0.87 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
3805 697.8171 1393.6196 1393.6217 -1.49 0 5 0.75 +1Score > 16 indicates identity MEQSVANLVDMR + 2 Deamidated (NQ)
5860 873.3969 1744.7792 1744.7839 -2.64 1 5 1.1 +1Score > 18 indicates identity GGQYCSADYQAQLKR + Deamidated (NQ)
3655 684.3109 1366.6072 1366.6299 -16.6 1 5 0.69 +1Score > 16 indicates identity SRDAFIDEMQR
5005 792.3600 1582.7054 1582.6987 4.26 0 5 0.52 +1Score > 15 indicates identity WEVHNFTMGYQR + Oxidation (M)
284 400.6820 799.3494 799.3573 -9.78 0 5 0.32 +1Score > 13 indicates identity HAGNSGTR + Deamidated (NQ)
2060 549.2496 1096.4846 1096.4978 -12.0 0 5 0.55 +1Score > 15 indicates identity YYGYTGAFR
766 441.2004 880.3862 880.3927 -7.31 0 5 0.52 +1Score > 15 indicates identity DGDGFGVSK
4404 751.8416 1501.6686 1501.6943 -17.1 1 5 0.87 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
2753 603.2805 1204.5464 1204.5684 -18.2 0 5 0.96 +1Score > 17 indicates identity SAETLSGGEAQR
265 400.6820 799.3494 799.3573 -9.78 0 5 0.33 +1Score > 13 indicates identity HAGNSGTR + Deamidated (NQ)
1686 522.2372 1042.4598 1042.4791 -18.5 1 5 0.33 +1Score > 13 indicates identity ANNPDRAER + Deamidated (NQ)
2850 616.7802 1231.5458 1231.5503 -3.62 0 5 0.58 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
7076 967.9399 1933.8652 1933.8298 18.3 1 5 0.89 +1Score > 17 indicates identity QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M)
5922 873.3969 1744.7792 1744.7735 3.28 0 5 1.2 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
4834 778.8539 1555.6932 1555.7163 -14.8 0 5 0.64 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
562 427.6943 853.3740 853.3600 16.5 0 5 0.34 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
2296 576.2618 1150.5090 1150.5254 -14.2 1 5 0.63 +1Score > 15 indicates identity LEFNNDNRK + 2 Deamidated (NQ)
6411 913.9153 1825.8160 1825.8186 -1.39 1 5 0.95 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
2550 589.7679 1177.5212 1177.5107 8.95 0 5 0.5 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
3127 643.7925 1285.5704 1285.5761 -4.41 0 5 0.57 +1Score > 15 indicates identity MQAYFDQLDR
6904 954.4337 1906.8528 1906.8690 -8.48 1 5 1 +1Score > 17 indicates identity LSAEDNARVNVAMNQTR + 3 Deamidated (NQ); Oxidation (M)
2223 562.7557 1123.4968 1123.4824 12.9 1 5 0.53 +1Score > 14 indicates identity RQMMGMEPK + Deamidated (NQ); Oxidation (M)
2955 630.2834 1258.5522 1258.5621 -7.79 0 5 0.35 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
2227 562.7557 1123.4968 1123.4968 0.033 0 5 0.54 +1Score > 14 indicates identity MASGVDPFER + Oxidation (M)
4802 778.8539 1555.6932 1555.7163 -14.8 0 5 0.66 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
4803 778.8539 1555.6932 1555.7163 -14.8 0 5 0.66 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
2894 616.7802 1231.5458 1231.5503 -3.62 0 5 0.62 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
3621 684.3109 1366.6072 1366.6299 -16.6 1 5 0.78 +1Score > 16 indicates identity GAAMEGQYKGAQR + Deamidated (NQ)
69 387.1759 772.3372 772.3351 2.72 0 4 0.36 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
4789 778.8539 1555.6932 1555.7163 -14.8 0 4 0.69 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
7028 967.9399 1933.8652 1933.8993 -17.6 1 4 0.96 +1Score > 17 indicates identity LSGFDMGYSGIQFGGDKR
632 427.6943 853.3740 853.3851 -13.0 0 4 0.37 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
2969 630.2841 1258.5536 1258.5621 -6.68 0 4 0.37 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
4836 778.8539 1555.6932 1555.7163 -14.8 0 4 0.7 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
2971 630.2841 1258.5536 1258.5724 -14.9 1 4 0.37 +1Score > 13 indicates identity TAMGEPGPDGRR + Oxidation (M)
4846 778.8539 1555.6932 1555.7163 -14.8 0 4 0.71 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
3962 711.3232 1420.6318 1420.6187 9.25 1 4 0.92 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
4261 738.3355 1474.6564 1474.6398 11.3 0 4 0.73 +1Score > 15 indicates identity YLYANMEVNGQR + 2 Deamidated (NQ); Oxidation (M)
4345 738.3355 1474.6564 1474.6398 11.3 0 4 0.74 +1Score > 15 indicates identity YLYANMEVNGQR + 2 Deamidated (NQ); Oxidation (M)
4432 751.8416 1501.6686 1501.6909 -14.8 1 4 1 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
4503 751.8416 1501.6686 1501.6727 -2.71 1 4 1 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
1440 495.2250 988.4354 988.4461 -10.8 0 4 0.38 +1Score > 13 indicates identity EELDQEAR
3708 684.3109 1366.6072 1366.6266 -14.1 1 4 0.84 +1Score > 16 indicates identity DWRETNIDYR
5559 846.3846 1690.7546 1690.7403 8.49 0 4 0.81 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
2502 589.7679 1177.5212 1177.5363 -12.8 1 4 0.56 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
2851 616.7802 1231.5458 1231.5615 -12.7 1 4 0.68 +1Score > 15 indicates identity IREMSDNDPR
6430 913.9153 1825.8160 1825.8482 -17.6 1 4 1.1 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
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