MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1098 468.2127 934.4108
1099 468.2127 934.4108
1100 468.2127 934.4108
1101 468.2127 934.4108
1102 468.2127 934.4108
1103 468.2127 934.4108
1104 468.2127 934.4108
1105 468.2127 934.4108
1106 468.2127 934.4108
1107 468.2127 934.4108
1108 468.2127 934.4108
1109 468.2127 934.4108
1110 468.2127 934.4108
1111 468.2127 934.4108
1112 468.2127 934.4108
1113 468.2127 934.4108
1114 468.2127 934.4108
1115 468.2127 934.4108
1116 468.2127 934.4108
1118 468.2127 934.4108
1119 468.2127 934.4108
1122 468.2127 934.4108
1123 468.2127 934.4108
1124 468.2127 934.4108
1125 468.2127 934.4108
1126 468.2127 934.4108
1127 468.2127 934.4108
1128 468.2127 934.4108
1129 468.2127 934.4108
1130 468.2127 934.4108
1131 468.2127 934.4108
1132 468.2127 934.4108
1133 468.2127 934.4108
1134 468.2143 934.4140
1135 481.7188 961.4230
1136 481.7188 961.4230
1137 481.7188 961.4230
1138 481.7188 961.4230
1139 481.7188 961.4230
1140 481.7188 961.4230
1141 481.7188 961.4230
1142 481.7188 961.4230
1143 481.7188 961.4230
1144 481.7188 961.4230
1145 481.7188 961.4230
1146 481.7188 961.4230
1147 481.7188 961.4230
1148 481.7188 961.4230
1149 481.7188 961.4230
1150 481.7188 961.4230
1151 481.7188 961.4230
1152 481.7188 961.4230
1153 481.7188 961.4230
1154 481.7188 961.4230
1155 481.7188 961.4230
1156 481.7188 961.4230
1157 481.7188 961.4230
1158 481.7188 961.4230
1159 481.7188 961.4230
1160 481.7188 961.4230
1161 481.7188 961.4230
1163 481.7188 961.4230
1165 481.7188 961.4230
1167 481.7188 961.4230
1168 481.7188 961.4230
1169 481.7188 961.4230
1170 481.7188 961.4230
1171 481.7188 961.4230
1173 481.7188 961.4230
1174 481.7188 961.4230
1177 481.7188 961.4230
1178 481.7188 961.4230
1179 481.7188 961.4230
1181 481.7188 961.4230
1183 481.7188 961.4230
1184 481.7188 961.4230
1185 481.7188 961.4230
1186 481.7188 961.4230
1188 481.7188 961.4230
1189 481.7188 961.4230
1190 481.7188 961.4230
1191 481.7188 961.4230
1192 481.7188 961.4230
1193 481.7188 961.4230
1195 481.7188 961.4230
1196 481.7188 961.4230
1199 481.7188 961.4230
1200 481.7188 961.4230
1201 481.7188 961.4230
1204 481.7188 961.4230
1205 481.7188 961.4230
1206 481.7188 961.4230
1207 481.7188 961.4230
1208 481.7188 961.4230
1210 481.7188 961.4230
1211 481.7188 961.4230
1212 481.7188 961.4230
1213 481.7188 961.4230
1215 481.7188 961.4230
1217 481.7188 961.4230
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