MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 7204)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
982 468.2127 934.4108
983 468.2127 934.4108
985 468.2127 934.4108
986 468.2127 934.4108
987 468.2127 934.4108
988 468.2127 934.4108
989 468.2127 934.4108
990 468.2127 934.4108
991 468.2127 934.4108
992 468.2127 934.4108
993 468.2127 934.4108
994 468.2127 934.4108
996 468.2127 934.4108
997 468.2127 934.4108
998 468.2127 934.4108
999 468.2127 934.4108
1000 468.2127 934.4108
1001 468.2127 934.4108
1002 468.2127 934.4108
1003 468.2127 934.4108
1004 468.2127 934.4108
1005 468.2127 934.4108
1006 468.2127 934.4108
1007 468.2127 934.4108
1008 468.2127 934.4108
1009 468.2127 934.4108
1010 468.2127 934.4108
1011 468.2127 934.4108
1012 468.2127 934.4108
1013 468.2127 934.4108
1014 468.2127 934.4108
1015 468.2127 934.4108
1016 468.2127 934.4108
1017 468.2127 934.4108
1018 468.2127 934.4108
1019 468.2127 934.4108
1020 468.2127 934.4108
1021 468.2127 934.4108
1023 468.2127 934.4108
1024 468.2127 934.4108
1025 468.2127 934.4108
1026 468.2127 934.4108
1027 468.2127 934.4108
1028 468.2127 934.4108
1029 468.2127 934.4108
1032 468.2127 934.4108
1033 468.2127 934.4108
1035 468.2127 934.4108
1036 468.2127 934.4108
1037 468.2127 934.4108
1038 468.2127 934.4108
1039 468.2127 934.4108
1040 468.2127 934.4108
1041 468.2127 934.4108
1042 468.2127 934.4108
1043 468.2127 934.4108
1044 468.2127 934.4108
1045 468.2127 934.4108
1046 468.2127 934.4108
1047 468.2127 934.4108
1048 468.2127 934.4108
1049 468.2127 934.4108
1050 468.2127 934.4108
1051 468.2127 934.4108
1052 468.2127 934.4108
1053 468.2127 934.4108
1054 468.2127 934.4108
1056 468.2127 934.4108
1058 468.2127 934.4108
1059 468.2127 934.4108
1060 468.2127 934.4108
1061 468.2127 934.4108
1062 468.2127 934.4108
1064 468.2127 934.4108
1065 468.2127 934.4108
1066 468.2127 934.4108
1067 468.2127 934.4108
1068 468.2127 934.4108
1071 468.2127 934.4108
1072 468.2127 934.4108
1073 468.2127 934.4108
1074 468.2127 934.4108
1075 468.2127 934.4108
1076 468.2127 934.4108
1077 468.2127 934.4108
1078 468.2127 934.4108
1079 468.2127 934.4108
1080 468.2127 934.4108
1081 468.2127 934.4108
1082 468.2127 934.4108
1083 468.2127 934.4108
1084 468.2127 934.4108
1085 468.2127 934.4108
1087 468.2127 934.4108
1088 468.2127 934.4108
1089 468.2127 934.4108
1091 468.2127 934.4108
1094 468.2127 934.4108
1095 468.2127 934.4108
1096 468.2127 934.4108
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Not what you expected? Try the select summary.