MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
764 441.2004 880.3862
765 441.2004 880.3862
767 441.2004 880.3862
768 441.2004 880.3862
769 441.2004 880.3862
771 441.2004 880.3862
772 441.2004 880.3862
773 441.2004 880.3862
774 441.2004 880.3862
775 441.2004 880.3862
776 441.2004 880.3862
777 441.2004 880.3862
778 441.2004 880.3862
779 441.2004 880.3862
780 441.2004 880.3862
781 441.2004 880.3862
782 441.2004 880.3862
783 441.2004 880.3862
785 441.2004 880.3862
786 441.2004 880.3862
787 441.2004 880.3862
788 441.2004 880.3862
790 441.2004 880.3862
791 441.2004 880.3862
792 441.2004 880.3862
793 441.2004 880.3862
795 441.2004 880.3862
796 441.2004 880.3862
797 441.2004 880.3862
798 441.2004 880.3862
799 441.2004 880.3862
800 441.2004 880.3862
801 441.2004 880.3862
802 441.2004 880.3862
803 441.2004 880.3862
804 441.2004 880.3862
805 441.2004 880.3862
806 441.2004 880.3862
807 441.2043 880.3940
809 441.2086 880.4026
810 441.2092 880.4038
811 454.7066 907.3986
812 454.7066 907.3986
813 454.7066 907.3986
814 454.7066 907.3986
815 454.7066 907.3986
816 454.7066 907.3986
817 454.7066 907.3986
818 454.7066 907.3986
819 454.7066 907.3986
820 454.7066 907.3986
821 454.7066 907.3986
822 454.7066 907.3986
823 454.7066 907.3986
824 454.7066 907.3986
825 454.7066 907.3986
826 454.7066 907.3986
827 454.7066 907.3986
828 454.7066 907.3986
829 454.7066 907.3986
830 454.7066 907.3986
831 454.7066 907.3986
832 454.7066 907.3986
833 454.7066 907.3986
834 454.7066 907.3986
835 454.7066 907.3986
837 454.7066 907.3986
838 454.7066 907.3986
839 454.7066 907.3986
840 454.7066 907.3986
841 454.7066 907.3986
842 454.7066 907.3986
843 454.7066 907.3986
844 454.7066 907.3986
845 454.7066 907.3986
846 454.7066 907.3986
847 454.7066 907.3986
848 454.7066 907.3986
849 454.7066 907.3986
850 454.7066 907.3986
851 454.7066 907.3986
852 454.7066 907.3986
853 454.7066 907.3986
854 454.7066 907.3986
855 454.7066 907.3986
856 454.7066 907.3986
857 454.7066 907.3986
858 454.7066 907.3986
859 454.7066 907.3986
860 454.7066 907.3986
861 454.7066 907.3986
862 454.7066 907.3986
863 454.7066 907.3986
864 454.7066 907.3986
865 454.7066 907.3986
866 454.7066 907.3986
867 454.7066 907.3986
868 454.7066 907.3986
869 454.7066 907.3986
870 454.7066 907.3986
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