MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
631 427.6943 853.3740
633 427.6943 853.3740
634 427.6943 853.3740
635 427.6943 853.3740
637 427.6943 853.3740
638 427.6943 853.3740
639 427.6943 853.3740
640 427.6943 853.3740
641 427.6943 853.3740
642 427.6943 853.3740
643 427.6943 853.3740
644 427.6943 853.3740
645 427.6943 853.3740
646 427.6943 853.3740
647 427.6943 853.3740
648 427.6943 853.3740
650 441.1925 880.3704
652 441.1925 880.3704
653 441.1926 880.3706
655 441.1928 880.3710
661 441.1931 880.3716
666 441.1932 880.3718
669 441.1933 880.3720
672 441.1934 880.3722
673 441.1934 880.3722
674 441.1935 880.3724
675 441.1935 880.3724
676 441.1935 880.3724
677 441.1935 880.3724
679 441.1936 880.3726
680 441.1938 880.3730
681 441.1938 880.3730
682 441.1939 880.3732
684 441.1939 880.3732
686 441.1941 880.3736
687 441.1941 880.3736
688 441.1942 880.3738
693 441.1942 880.3738
694 441.1942 880.3738
695 441.1942 880.3738
696 441.1943 880.3740
698 441.1944 880.3742
699 441.1944 880.3742
702 441.1946 880.3746
703 441.1947 880.3748
704 441.1948 880.3750
706 441.1954 880.3762
707 441.1996 880.3846
708 441.2004 880.3862
709 441.2004 880.3862
710 441.2004 880.3862
711 441.2004 880.3862
712 441.2004 880.3862
713 441.2004 880.3862
714 441.2004 880.3862
715 441.2004 880.3862
716 441.2004 880.3862
717 441.2004 880.3862
718 441.2004 880.3862
719 441.2004 880.3862
720 441.2004 880.3862
721 441.2004 880.3862
722 441.2004 880.3862
723 441.2004 880.3862
724 441.2004 880.3862
725 441.2004 880.3862
726 441.2004 880.3862
727 441.2004 880.3862
728 441.2004 880.3862
729 441.2004 880.3862
730 441.2004 880.3862
731 441.2004 880.3862
732 441.2004 880.3862
733 441.2004 880.3862
734 441.2004 880.3862
735 441.2004 880.3862
736 441.2004 880.3862
737 441.2004 880.3862
738 441.2004 880.3862
739 441.2004 880.3862
740 441.2004 880.3862
741 441.2004 880.3862
742 441.2004 880.3862
743 441.2004 880.3862
744 441.2004 880.3862
745 441.2004 880.3862
746 441.2004 880.3862
747 441.2004 880.3862
748 441.2004 880.3862
749 441.2004 880.3862
750 441.2004 880.3862
752 441.2004 880.3862
753 441.2004 880.3862
754 441.2004 880.3862
756 441.2004 880.3862
757 441.2004 880.3862
758 441.2004 880.3862
759 441.2004 880.3862
761 441.2004 880.3862
763 441.2004 880.3862
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