MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
519 427.6943 853.3740
520 427.6943 853.3740
521 427.6943 853.3740
522 427.6943 853.3740
523 427.6943 853.3740
524 427.6943 853.3740
525 427.6943 853.3740
526 427.6943 853.3740
527 427.6943 853.3740
528 427.6943 853.3740
529 427.6943 853.3740
530 427.6943 853.3740
531 427.6943 853.3740
532 427.6943 853.3740
533 427.6943 853.3740
534 427.6943 853.3740
535 427.6943 853.3740
537 427.6943 853.3740
538 427.6943 853.3740
539 427.6943 853.3740
540 427.6943 853.3740
541 427.6943 853.3740
542 427.6943 853.3740
543 427.6943 853.3740
544 427.6943 853.3740
545 427.6943 853.3740
546 427.6943 853.3740
547 427.6943 853.3740
549 427.6943 853.3740
550 427.6943 853.3740
551 427.6943 853.3740
552 427.6943 853.3740
553 427.6943 853.3740
554 427.6943 853.3740
555 427.6943 853.3740
556 427.6943 853.3740
557 427.6943 853.3740
558 427.6943 853.3740
559 427.6943 853.3740
560 427.6943 853.3740
561 427.6943 853.3740
563 427.6943 853.3740
564 427.6943 853.3740
565 427.6943 853.3740
566 427.6943 853.3740
567 427.6943 853.3740
570 427.6943 853.3740
571 427.6943 853.3740
573 427.6943 853.3740
574 427.6943 853.3740
576 427.6943 853.3740
577 427.6943 853.3740
578 427.6943 853.3740
579 427.6943 853.3740
581 427.6943 853.3740
582 427.6943 853.3740
583 427.6943 853.3740
584 427.6943 853.3740
585 427.6943 853.3740
586 427.6943 853.3740
587 427.6943 853.3740
588 427.6943 853.3740
590 427.6943 853.3740
591 427.6943 853.3740
592 427.6943 853.3740
593 427.6943 853.3740
594 427.6943 853.3740
595 427.6943 853.3740
596 427.6943 853.3740
597 427.6943 853.3740
598 427.6943 853.3740
599 427.6943 853.3740
600 427.6943 853.3740
602 427.6943 853.3740
603 427.6943 853.3740
604 427.6943 853.3740
605 427.6943 853.3740
606 427.6943 853.3740
607 427.6943 853.3740
609 427.6943 853.3740
610 427.6943 853.3740
611 427.6943 853.3740
612 427.6943 853.3740
613 427.6943 853.3740
614 427.6943 853.3740
615 427.6943 853.3740
616 427.6943 853.3740
617 427.6943 853.3740
618 427.6943 853.3740
619 427.6943 853.3740
620 427.6943 853.3740
621 427.6943 853.3740
622 427.6943 853.3740
624 427.6943 853.3740
625 427.6943 853.3740
626 427.6943 853.3740
627 427.6943 853.3740
628 427.6943 853.3740
629 427.6943 853.3740
630 427.6943 853.3740
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