MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
395 414.1881 826.3616
396 414.1881 826.3616
397 414.1881 826.3616
398 414.1881 826.3616
399 414.1881 826.3616
400 414.1881 826.3616
402 414.1881 826.3616
403 414.1881 826.3616
404 414.1881 826.3616
405 414.1881 826.3616
406 414.1881 826.3616
407 414.1881 826.3616
408 414.1881 826.3616
409 414.1881 826.3616
412 414.1881 826.3616
413 414.1881 826.3616
414 414.1881 826.3616
415 414.1881 826.3616
416 414.1881 826.3616
419 414.1881 826.3616
420 414.1881 826.3616
423 414.1881 826.3616
427 414.1881 826.3616
428 414.1881 826.3616
430 414.1881 826.3616
431 414.1881 826.3616
433 414.1881 826.3616
434 414.1881 826.3616
436 414.1881 826.3616
437 414.1881 826.3616
438 414.1881 826.3616
441 414.1881 826.3616
442 414.1881 826.3616
443 414.1881 826.3616
445 414.1881 826.3616
446 414.1881 826.3616
451 414.1881 826.3616
453 414.1881 826.3616
454 414.1881 826.3616
456 414.1881 826.3616
457 414.1881 826.3616
459 414.1881 826.3616
460 414.1881 826.3616
461 414.1881 826.3616
462 414.1881 826.3616
463 414.1881 826.3616
464 414.1881 826.3616
466 414.1881 826.3616
467 414.1881 826.3616
468 414.1881 826.3616
469 414.1881 826.3616
470 414.1881 826.3616
471 414.1881 826.3616
472 414.1881 826.3616
473 414.1881 826.3616
474 414.1881 826.3616
475 414.1881 826.3616
476 414.1881 826.3616
477 414.1881 826.3616
478 414.1881 826.3616
479 414.1881 826.3616
480 414.1881 826.3616
481 414.1881 826.3616
482 414.1881 826.3616
483 414.1881 826.3616
484 414.1881 826.3616
485 414.1881 826.3616
486 414.1921 826.3696
487 427.6943 853.3740
488 427.6943 853.3740
489 427.6943 853.3740
490 427.6943 853.3740
491 427.6943 853.3740
492 427.6943 853.3740
493 427.6943 853.3740
494 427.6943 853.3740
495 427.6943 853.3740
496 427.6943 853.3740
497 427.6943 853.3740
498 427.6943 853.3740
499 427.6943 853.3740
500 427.6943 853.3740
501 427.6943 853.3740
502 427.6943 853.3740
503 427.6943 853.3740
504 427.6943 853.3740
505 427.6943 853.3740
506 427.6943 853.3740
507 427.6943 853.3740
508 427.6943 853.3740
509 427.6943 853.3740
510 427.6943 853.3740
511 427.6943 853.3740
512 427.6943 853.3740
513 427.6943 853.3740
514 427.6943 853.3740
515 427.6943 853.3740
516 427.6943 853.3740
517 427.6943 853.3740
518 427.6943 853.3740
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