MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
290 400.6820 799.3494
291 400.6820 799.3494
292 400.6820 799.3494
293 400.6820 799.3494
294 400.6820 799.3494
295 400.6820 799.3494
296 400.6820 799.3494
297 400.6820 799.3494
298 400.6820 799.3494
299 400.6820 799.3494
300 400.6820 799.3494
301 400.6820 799.3494
302 400.6820 799.3494
303 400.6820 799.3494
304 400.6820 799.3494
305 400.6820 799.3494
306 400.6820 799.3494
307 400.6820 799.3494
308 400.6820 799.3494
309 400.6820 799.3494
310 400.6820 799.3494
311 400.6820 799.3494
312 400.6820 799.3494
313 400.6820 799.3494
314 400.6820 799.3494
315 400.6820 799.3494
316 400.6820 799.3494
317 400.6820 799.3494
318 400.6820 799.3494
319 400.6820 799.3494
320 400.6820 799.3494
321 400.6820 799.3494
322 400.6821 799.3496
323 400.6832 799.3518
324 400.6868 799.3590
326 414.1805 826.3464
327 414.1811 826.3476
328 414.1815 826.3484
329 414.1816 826.3486
330 414.1816 826.3486
331 414.1817 826.3488
332 414.1819 826.3492
333 414.1820 826.3494
334 414.1827 826.3508
335 414.1829 826.3512
336 414.1832 826.3518
337 414.1832 826.3518
338 414.1832 826.3518
339 414.1835 826.3524
340 414.1837 826.3528
341 414.1838 826.3530
342 414.1839 826.3532
343 414.1840 826.3534
344 414.1840 826.3534
345 414.1841 826.3536
346 414.1841 826.3536
347 414.1842 826.3538
350 414.1845 826.3544
351 414.1846 826.3546
352 414.1849 826.3552
354 414.1856 826.3566
355 414.1859 826.3572
356 414.1869 826.3592
357 414.1869 826.3592
358 414.1873 826.3600
359 414.1873 826.3600
360 414.1880 826.3614
361 414.1881 826.3616
362 414.1881 826.3616
363 414.1881 826.3616
364 414.1881 826.3616
365 414.1881 826.3616
366 414.1881 826.3616
367 414.1881 826.3616
368 414.1881 826.3616
369 414.1881 826.3616
370 414.1881 826.3616
371 414.1881 826.3616
372 414.1881 826.3616
373 414.1881 826.3616
374 414.1881 826.3616
375 414.1881 826.3616
376 414.1881 826.3616
377 414.1881 826.3616
378 414.1881 826.3616
379 414.1881 826.3616
380 414.1881 826.3616
381 414.1881 826.3616
382 414.1881 826.3616
383 414.1881 826.3616
384 414.1881 826.3616
385 414.1881 826.3616
386 414.1881 826.3616
387 414.1881 826.3616
388 414.1881 826.3616
389 414.1881 826.3616
390 414.1881 826.3616
392 414.1881 826.3616
393 414.1881 826.3616
394 414.1881 826.3616
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