MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
79 387.1759 772.3372
80 387.1759 772.3372
81 387.1759 772.3372
82 387.1759 772.3372
83 387.1759 772.3372
84 387.1759 772.3372
85 387.1771 772.3396
87 387.1788 772.3430
88 387.1789 772.3432
89 387.1793 772.3440
90 387.1796 772.3446
91 387.1797 772.3448
92 387.1797 772.3448
93 387.1799 772.3452
94 387.1799 772.3452
95 387.1799 772.3452
96 387.1799 772.3452
97 387.1799 772.3452
99 387.1800 772.3454
100 387.1801 772.3456
101 387.1801 772.3456
103 387.1801 772.3456
104 387.1801 772.3456
105 387.1802 772.3458
106 387.1802 772.3458
107 387.1802 772.3458
108 387.1802 772.3458
109 387.1803 772.3460
111 387.1803 772.3460
112 387.1804 772.3462
113 387.1804 772.3462
114 387.1804 772.3462
115 387.1804 772.3462
116 387.1804 772.3462
117 387.1805 772.3464
118 387.1805 772.3464
119 387.1805 772.3464
120 387.1805 772.3464
121 387.1805 772.3464
122 387.1805 772.3464
123 387.1805 772.3464
124 387.1805 772.3464
125 387.1805 772.3464
126 387.1806 772.3466
127 387.1806 772.3466
128 387.1806 772.3466
129 387.1806 772.3466
130 387.1806 772.3466
131 387.1806 772.3466
132 387.1806 772.3466
134 387.1807 772.3468
135 387.1807 772.3468
136 387.1807 772.3468
137 387.1807 772.3468
138 387.1807 772.3468
139 387.1808 772.3470
140 387.1808 772.3470
142 387.1808 772.3470
143 387.1808 772.3470
144 387.1809 772.3472
145 387.1809 772.3472
146 387.1810 772.3474
147 387.1811 772.3476
148 387.1811 772.3476
150 387.1811 772.3476
151 387.1812 772.3478
152 387.1812 772.3478
153 387.1812 772.3478
154 387.1813 772.3480
156 387.1813 772.3480
157 387.1815 772.3484
158 387.1816 772.3486
159 387.1817 772.3488
160 387.1821 772.3496
161 387.1822 772.3498
162 387.1823 772.3500
163 400.6772 799.3398
164 400.6773 799.3400
165 400.6780 799.3414
166 400.6784 799.3422
167 400.6812 799.3478
168 400.6819 799.3492
169 400.6820 799.3494
170 400.6820 799.3494
171 400.6820 799.3494
172 400.6820 799.3494
173 400.6820 799.3494
174 400.6820 799.3494
175 400.6820 799.3494
176 400.6820 799.3494
177 400.6820 799.3494
178 400.6820 799.3494
179 400.6820 799.3494
180 400.6820 799.3494
181 400.6820 799.3494
182 400.6820 799.3494
183 400.6820 799.3494
184 400.6820 799.3494
185 400.6820 799.3494
186 400.6820 799.3494
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