MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | PRT1377_JBEI_2 |
| MS data file | : | PRT1377_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 23 Jan 2026 at 21:31:54 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,287 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| Score | Mass | Matches | Sequences | emPAI | |||
| 1 | SYS_ECOLI | 56 | 48669 | 3 (2) | 1 (1) | 0.07 | Serine--tRNA ligase OS=Escherichia coli (strain K12) GN=serS PE=1 SV=1 |
| 2 | Q88JG6_PSEPK | 26 | 61597 | 2 (2) | 1 (1) | 0.05 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
| 3 | GLCF_ECOLI | 21 | 46051 | 6 (1) | 1 (1) | 0.07 | Glycolate oxidase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=glcF PE=4 SV=1 |
| 4 | CCMF_ECOLI | 17 | 71571 | 39 (1) | 1 (1) | 0.05 | Cytochrome c-type biogenesis protein CcmF OS=Escherichia coli (strain K12) GN=ccmF PE=1 SV=1 |
| 5 | RS2_ECOLI | 14 | 26784 | 27 (1) | 1 (1) | 0.12 | 30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2 |
| 6 | TRMH_ECOLI | 14 | 25498 | 11 (1) | 1 (1) | 0.13 | tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1 |
| 7 | HOFB_ECOLI | 14 | 51154 | 3 (1) | 1 (1) | 0.06 | Protein transport protein HofB homolog OS=Escherichia coli (strain K12) GN=hofB PE=3 SV=3 |
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