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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5322 819.3723 1636.7300 1636.7403 -6.24 0 16 0.067 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
5295 819.3723 1636.7300 1636.7403 -6.24 0 14 0.1 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
4492 751.8416 1501.6686 1501.6727 -2.71 1 13 0.13 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
2093 549.2496 1096.4846 1096.4978 -12.0 0 13 0.081 +1Score > 15 indicates identity YYGYTGAFR
2350 576.2618 1150.5090 1150.5254 -14.2 1 13 0.089 -1Score > 15 indicates identity LEFNNDNRK + 2 Deamidated (NQ)
3705 684.3109 1366.6072 1366.6252 -13.1 0 13 0.12 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
2086 549.2496 1096.4846 1096.4978 -12.0 0 12 0.097 +1Score > 15 indicates identity YYGYTGAFR
3714 684.3109 1366.6072 1366.6252 -13.1 0 12 0.14 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
902 454.7066 907.3986 907.3957 3.29 0 12 0.089 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
2962 630.2836 1258.5526 1258.5621 -7.48 0 12 0.069 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
4767 778.8539 1555.6932 1555.7163 -14.8 0 12 0.13 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
1734 522.2372 1042.4598 1042.4576 2.17 0 11 0.074 +1Score > 13 indicates identity GFADAIMMR + 2 Oxidation (M)
930 454.7066 907.3986 907.3957 3.29 0 11 0.1 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
683 441.1939 880.3732 880.3562 19.3 0 11 0.097 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4192 724.8293 1447.6440 1447.6725 -19.7 1 11 0.098 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
3017 630.2864 1258.5582 1258.5367 17.1 0 11 0.11 +1Score > 14 indicates identity QWNNEPAGWR + 2 Deamidated (NQ)
4799 778.8539 1555.6932 1555.6759 11.2 0 11 0.16 +1Score > 15 indicates identity LNAMGAFAEAQTCR + Deamidated (NQ); Oxidation (M)
3715 684.3109 1366.6072 1366.6252 -13.1 0 11 0.19 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
3677 684.3109 1366.6072 1366.6252 -13.1 0 10 0.2 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
984 468.2127 934.4108 934.4178 -7.46 1 10 0.17 +1Score > 15 indicates identity LDEDCKR
5113 805.8662 1609.7178 1609.7294 -7.16 0 10 0.17 +1Score > 15 indicates identity EALAMVDLEGFEDR + Oxidation (M)
1552 508.7311 1015.4476 1015.4611 -13.2 0 10 0.11 +1Score > 13 indicates identity YYDAETVR
572 427.6943 853.3740 853.3851 -13.0 0 10 0.095 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
670 441.1933 880.3720 880.3562 17.9 0 10 0.12 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
6007 886.9030 1771.7914 1771.7584 18.7 1 10 0.22 +1Score > 16 indicates identity DPASDQMQHWKEQR + Deamidated (NQ); Oxidation (M)
2454 589.7679 1177.5212 1177.5332 -10.1 1 10 0.14 +1Score > 14 indicates identity ANNPTSRMMR + Deamidated (NQ)
2472 589.7679 1177.5212 1177.5363 -12.8 1 10 0.15 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
4196 724.8293 1447.6440 1447.6725 -19.7 1 10 0.13 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
4960 792.3600 1582.7054 1582.6975 5.00 0 10 0.18 +1Score > 15 indicates identity QEVCAMMALNMLR + Deamidated (NQ); Oxidation (M)
751 441.2004 880.3862 880.3926 -7.26 0 9 0.18 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
1093 468.2127 934.4108 934.4178 -7.46 1 9 0.22 +1Score > 15 indicates identity LDEDCKR
697 441.1944 880.3742 880.3596 16.6 0 9 0.13 +1Score > 13 indicates identity LNSQMNR + 3 Deamidated (NQ); Oxidation (M)
2217 562.7557 1123.4968 1123.4974 -0.52 0 9 0.19 +1Score > 14 indicates identity FYEYYSPR
2173 562.7557 1123.4968 1123.4894 6.65 0 9 0.19 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
2859 616.7802 1231.5458 1231.5680 -18.0 0 9 0.22 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
5768 859.8907 1717.7668 1717.7876 -12.1 1 9 0.23 +1Score > 15 indicates identity CRAEEPALNMLADGR + Oxidation (M)
689 441.1942 880.3738 880.3562 20.0 0 9 0.16 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
660 441.1930 880.3714 880.3562 17.3 0 9 0.15 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4815 778.8539 1555.6932 1555.6759 11.2 0 9 0.24 +1Score > 15 indicates identity LNAMGAFAEAQTCR + Deamidated (NQ); Oxidation (M)
5346 819.3723 1636.7300 1636.7403 -6.24 0 9 0.32 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
5345 819.3723 1636.7300 1636.7403 -6.24 0 9 0.32 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
2510 589.7679 1177.5212 1177.5107 8.95 0 9 0.19 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
1851 535.7434 1069.4722 1069.4862 -13.1 0 9 0.13 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2046 549.2496 1096.4846 1096.4924 -7.07 0 9 0.22 +1Score > 15 indicates identity EITEQYEGK + Deamidated (NQ)
2892 616.7802 1231.5458 1231.5503 -3.62 0 9 0.23 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
3319 657.2986 1312.5826 1312.5870 -3.33 0 9 0.29 +1Score > 16 indicates identity SYITGEFGHMR + Oxidation (M)
2055 549.2496 1096.4846 1096.4978 -12.0 0 9 0.23 +1Score > 15 indicates identity YYGYTGAFR
2823 616.7802 1231.5458 1231.5503 -3.62 0 9 0.24 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
5256 819.3723 1636.7300 1636.7403 -6.24 0 9 0.34 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
2640 603.2741 1204.5336 1204.5394 -4.75 0 9 0.31 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
1097 468.2127 934.4108 934.4178 -7.46 1 8 0.27 +1Score > 15 indicates identity LDEDCKR
2051 549.2496 1096.4846 1096.4978 -12.0 0 8 0.24 +1Score > 15 indicates identity YYGYTGAFR
6316 900.4092 1798.8038 1798.8196 -8.76 0 8 0.26 +1Score > 15 indicates identity TEGATVFGYQVMDPER
5762 859.8907 1717.7668 1717.7875 -12.0 1 8 0.27 +1Score > 15 indicates identity AEDMAAANAMASHLRK + 2 Oxidation (M)
589 427.6943 853.3740 853.3851 -13.0 0 8 0.15 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
3359 657.2986 1312.5826 1312.5935 -8.28 0 8 0.33 +1Score > 16 indicates identity TIAGEYPDYQR + Deamidated (NQ)
3859 697.8171 1393.6196 1393.6474 -19.9 1 8 0.38 +1Score > 16 indicates identity NVDKQTQDFAAR + 2 Deamidated (NQ)
6821 954.4337 1906.8528 1906.8796 -14.0 0 8 0.46 +1Score > 17 indicates identity TLAEGQNVEFEIQDGQK + 2 Deamidated (NQ)
668 441.1932 880.3718 880.3562 17.7 0 8 0.19 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
2521 589.7679 1177.5212 1177.5363 -12.8 1 8 0.23 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
6346 913.9153 1825.8160 1825.8087 4.02 1 8 0.45 +1Score > 17 indicates identity NGMECGIGVKNYNDVR + Deamidated (NQ)
1120 468.2127 934.4108 934.4178 -7.46 1 8 0.31 +1Score > 15 indicates identity LDEDCKR
5264 819.3723 1636.7300 1636.7119 11.1 1 8 0.4 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
4494 751.8416 1501.6686 1501.6727 -2.71 1 8 0.44 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
1883 535.7434 1069.4722 1069.4716 0.57 0 8 0.17 +1Score > 13 indicates identity DAGFQAFADK + Deamidated (NQ)
4311 738.3355 1474.6564 1474.6663 -6.70 0 8 0.32 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
2054 549.2496 1096.4846 1096.4715 12.0 0 8 0.28 +1Score > 15 indicates identity NMGGMASLMGK + Deamidated (NQ)
2876 616.7802 1231.5458 1231.5213 19.9 0 8 0.29 +1Score > 15 indicates identity MLISDGDYMR + 2 Oxidation (M)
3306 657.2986 1312.5826 1312.5643 13.9 1 8 0.37 +1Score > 16 indicates identity HDVKGDNEENR + Deamidated (NQ)
3325 657.2986 1312.5826 1312.5686 10.7 1 8 0.37 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
536 427.6943 853.3740 853.3851 -13.0 0 8 0.17 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
5806 859.8907 1717.7668 1717.7907 -13.9 1 7 0.33 +1Score > 15 indicates identity SVVNNDPQGYPNRVR + 4 Deamidated (NQ)
7110 967.9399 1933.8652 1933.8992 -17.6 1 7 0.48 +1Score > 17 indicates identity MSTPDNRSVNFFSLFR + Deamidated (NQ); Oxidation (M)
6685 940.9276 1879.8406 1879.8305 5.41 1 7 0.55 +1Score > 17 indicates identity QSHGNPLMKQGHQMQR + 4 Deamidated (NQ)
2145 562.7557 1123.4968 1123.4894 6.65 0 7 0.28 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
3054 630.2864 1258.5582 1258.5830 -19.6 1 7 0.25 +1Score > 14 indicates identity QVRSFTYDDK + Deamidated (NQ)
927 454.7066 907.3986 907.3957 3.29 0 7 0.26 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
1090 468.2127 934.4108 934.4178 -7.46 1 7 0.36 +1Score > 15 indicates identity LDEDCKR
3569 684.3032 1366.5918 1366.6153 -17.2 1 7 0.35 +1Score > 15 indicates identity FQQQKDWETR + 2 Deamidated (NQ)
4174 724.8293 1447.6440 1447.6725 -19.7 1 7 0.24 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
5431 832.8785 1663.7424 1663.7157 16.1 0 7 0.41 +1Score > 16 indicates identity VPPGMTMMYHAQER + Deamidated (NQ); Oxidation (M)
6018 886.9030 1771.7914 1771.7584 18.7 1 7 0.44 +1Score > 16 indicates identity DPASDQMQHWKEQR + Deamidated (NQ); Oxidation (M)
6832 954.4337 1906.8528 1906.8513 0.80 1 7 0.57 +1Score > 17 indicates identity DDVNEVRNGMECGIGVK + Oxidation (M)
1172 481.7188 961.4230 961.4253 -2.38 0 7 0.32 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
5783 859.8907 1717.7668 1717.7907 -13.9 1 7 0.37 +1Score > 15 indicates identity SVVNNDPQGYPNRVR + 4 Deamidated (NQ)
6423 913.9153 1825.8160 1825.8485 -17.8 1 7 0.57 +1Score > 17 indicates identity MTGRLALVMGAEGEGMR + 3 Oxidation (M)
1967 549.2496 1096.4846 1096.4978 -12.0 0 7 0.35 +1Score > 15 indicates identity YYGYTGAFR
4401 751.8416 1501.6686 1501.6943 -17.1 1 7 0.56 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
1166 481.7188 961.4230 961.4253 -2.38 0 7 0.33 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
1692 522.2372 1042.4598 1042.4576 2.17 0 7 0.21 +1Score > 13 indicates identity GFADAIMMR + 2 Oxidation (M)
3852 697.8171 1393.6196 1393.6217 -1.49 0 7 0.51 +1Score > 16 indicates identity MEQSVANLVDMR + 2 Deamidated (NQ)
7004 967.9399 1933.8652 1933.9017 -18.9 1 7 0.56 +1Score > 17 indicates identity QIYEDQLSSLHNDSKR + 2 Deamidated (NQ)
3642 684.3109 1366.6072 1366.6221 -10.8 0 7 0.47 +1Score > 16 indicates identity LANAAITDMQMR + Deamidated (NQ); 2 Oxidation (M)
325 414.1802 826.3458 826.3569 -13.4 0 7 0.22 +1Score > 13 indicates identity ANAQQHR + 3 Deamidated (NQ)
3261 657.2971 1312.5796 1312.5825 -2.19 0 7 0.46 +1Score > 16 indicates identity VSMPEITAMMR + 3 Oxidation (M)
3659 684.3109 1366.6072 1366.6252 -13.1 0 7 0.48 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
3858 697.8171 1393.6196 1393.6217 -1.49 0 7 0.52 +1Score > 16 indicates identity MEQSVANLVDMR + 2 Deamidated (NQ)
1216 481.7188 961.4230 961.4253 -2.38 0 7 0.35 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
4640 765.3478 1528.6810 1528.6576 15.3 0 7 0.35 +1Score > 15 indicates identity HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M)
575 427.6943 853.3740 853.3600 16.5 0 7 0.22 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
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