MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–7 (out of 7)

Page: 1 

+1

Accession Score Description
1 SYS_ECOLI 56 Serine--tRNA ligase OS=Escherichia coli (strain K12) GN=serS PE=1 SV=1

+2

Accession Score Description
1 Q88JG6_PSEPK 26 histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2

+3

Accession Score Description
1 GLCF_ECOLI 21 Glycolate oxidase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=glcF PE=4 SV=1

+4

Accession Score Description
1 CCMF_ECOLI 17 Cytochrome c-type biogenesis protein CcmF OS=Escherichia coli (strain K12) GN=ccmF PE=1 SV=1

-5

Accession Score Description
1 RS2_ECOLI 14 30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2
Score Mass Matches Sequences emPAI
5.1 RS2_ECOLI 14 26784 27 (1) 1 (1) 0.12
30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2

-27 peptide matches (1 non-duplicate, 26 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
401 -26 414.1881 826.3616 826.3677 -7.33 0 14 0.041 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
348   414.1843 826.3540 826.3677 -16.5 0 (1) 0.92 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
349   414.1844 826.3542 826.3677 -16.3 0 (1) 0.93 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
391   414.1881 826.3616 826.3677 -7.33 0 (6) 0.28 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
410   414.1881 826.3616 826.3677 -7.33 0 (2) 0.77 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
411   414.1881 826.3616 826.3677 -7.33 0 (3) 0.56 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
417   414.1881 826.3616 826.3677 -7.33 0 (1) 0.96 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
418   414.1881 826.3616 826.3677 -7.33 0 (0) 1.1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
421   414.1881 826.3616 826.3677 -7.33 0 (1) 0.85 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
422   414.1881 826.3616 826.3677 -7.33 0 (1) 0.87 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
424   414.1881 826.3616 826.3677 -7.33 0 (1) 0.82 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
425   414.1881 826.3616 826.3677 -7.33 0 (12) 0.079 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
426   414.1881 826.3616 826.3677 -7.33 0 (0) 1.1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
429   414.1881 826.3616 826.3677 -7.33 0 (0) 1.1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
432   414.1881 826.3616 826.3677 -7.33 0 (1) 0.99 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
435   414.1881 826.3616 826.3677 -7.33 0 (2) 0.78 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
439   414.1881 826.3616 826.3677 -7.33 0 (0) 1.1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
440   414.1881 826.3616 826.3677 -7.33 0 (1) 1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
444   414.1881 826.3616 826.3677 -7.33 0 (2) 0.8 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
447   414.1881 826.3616 826.3677 -7.33 0 (0) 1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
448   414.1881 826.3616 826.3677 -7.33 0 (1) 0.99 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
449   414.1881 826.3616 826.3677 -7.33 0 (3) 0.61 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
450   414.1881 826.3616 826.3677 -7.33 0 (5) 0.34 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
452   414.1881 826.3616 826.3677 -7.33 0 (2) 0.81 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
455   414.1881 826.3616 826.3677 -7.33 0 (7) 0.22 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
458   414.1881 826.3616 826.3677 -7.33 0 (0) 1 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)
465   414.1881 826.3616 826.3677 -7.33 0 (5) 0.37 +1Score > 13 indicates identity U -.MATVSMR.D + 2 Oxidation (M)

+6

Accession Score Description
1 TRMH_ECOLI 14 tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1

+7

Accession Score Description
1 HOFB_ECOLI 14 Protein transport protein HofB homolog OS=Escherichia coli (strain K12) GN=hofB PE=3 SV=3
Page: 1 

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