MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–7 (out of 7)

Page: 1 

+1

Accession Score Description
1 SYS_ECOLI 56 Serine--tRNA ligase OS=Escherichia coli (strain K12) GN=serS PE=1 SV=1

+2

Accession Score Description
1 Q88JG6_PSEPK 26 histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2

+3

Accession Score Description
1 GLCF_ECOLI 21 Glycolate oxidase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=glcF PE=4 SV=1

-4

Accession Score Description
1 CCMF_ECOLI 17 Cytochrome c-type biogenesis protein CcmF OS=Escherichia coli (strain K12) GN=ccmF PE=1 SV=1
Score Mass Matches Sequences emPAI
4.1 CCMF_ECOLI 17 71571 39 (1) 1 (1) 0.05
Cytochrome c-type biogenesis protein CcmF OS=Escherichia coli (strain K12) GN=ccmF PE=1 SV=1

-39 peptide matches (1 non-duplicate, 38 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2027 -38 549.2496 1096.4846 1096.4753 8.49 1 17 0.035 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1954   549.2496 1096.4846 1096.4753 8.49 1 (10) 0.15 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1958   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.89 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1959   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.87 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1960   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.3 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1961   549.2496 1096.4846 1096.4753 8.49 1 (4) 0.74 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1966   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.83 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1967   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.5 +2Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1968   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.2 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1972   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.1 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1987   549.2496 1096.4846 1096.4753 8.49 1 (0) 1.5 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
1990   549.2496 1096.4846 1096.4753 8.49 1 (0) 1.7 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2017   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.82 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2018   549.2496 1096.4846 1096.4753 8.49 1 (4) 0.75 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2020   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.8 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2021   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.93 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2034   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.4 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2035   549.2496 1096.4846 1096.4753 8.49 1 (3) 0.83 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2036   549.2496 1096.4846 1096.4753 8.49 1 (0) 1.7 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2038   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.4 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2045   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.2 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2047   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.1 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2052   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.1 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2055   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.3 +2Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2059   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.2 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2060   549.2496 1096.4846 1096.4753 8.49 1 (0) 1.5 +2Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2061   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.3 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2064   549.2496 1096.4846 1096.4753 8.49 1 (0) 1.6 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2068   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.1 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2069   549.2496 1096.4846 1096.4753 8.49 1 (2) 1.1 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2071   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.4 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2072   549.2496 1096.4846 1096.4753 8.49 1 (1) 1.4 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2082   549.2496 1096.4846 1096.4753 8.49 1 (11) 0.15 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2086   549.2496 1096.4846 1096.4753 8.49 1 (9) 0.21 +2Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2087   549.2496 1096.4846 1096.4753 8.49 1 (6) 0.42 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2093   549.2496 1096.4846 1096.4753 8.49 1 (12) 0.1 +2Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2104   549.2502 1096.4858 1096.4753 9.58 1 (1) 1.4 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2105   549.2505 1096.4864 1096.4753 10.1 1 (3) 0.95 +2Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)
2106   549.2516 1096.4886 1096.4753 12.1 1 (2) 0.96 +1Score > 15 indicates identity U R.GDARMMASSR.L + Oxidation (M)

+5

Accession Score Description
1 RS2_ECOLI 14 30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2

+6

Accession Score Description
1 TRMH_ECOLI 14 tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1

+7

Accession Score Description
1 HOFB_ECOLI 14 Protein transport protein HofB homolog OS=Escherichia coli (strain K12) GN=hofB PE=3 SV=3
Page: 1 

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