MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | PRT1377_JBEI_2 |
| MS data file | : | PRT1377_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 23 Jan 2026 at 21:31:54 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,287 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYS_ECOLI | 56 | Serine--tRNA ligase OS=Escherichia coli (strain K12) GN=serS PE=1 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 26 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 2.1 | Q88JG6_PSEPK |
26 | 61597 | 2 (2) | 1 (1) | 0.05 | |
| histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 | |||||||
2 peptide matches (1 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5742 |
1 |
859.8907 | 1717.7668 | 1717.7366 | 17.6 | 1 | 26 | 0.0045 | 1Score > 15 indicates identity |
U | R.NFLSNACRYTEEGR.I + 2 Deamidated (NQ) |
| 5737 |
859.8907 | 1717.7668 | 1717.7366 | 17.6 | 1 | (15) | 0.056 | 1Score > 15 indicates identity |
U | R.NFLSNACRYTEEGR.I + 2 Deamidated (NQ) |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLCF_ECOLI | 21 | Glycolate oxidase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=glcF PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CCMF_ECOLI | 17 | Cytochrome c-type biogenesis protein CcmF OS=Escherichia coli (strain K12) GN=ccmF PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_ECOLI | 14 | 30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRMH_ECOLI | 14 | tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HOFB_ECOLI | 14 | Protein transport protein HofB homolog OS=Escherichia coli (strain K12) GN=hofB PE=3 SV=3 |
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