MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2855 616.7802 1231.5458 1231.5503 -3.62 0 0 1.7 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
3814 697.8171 1393.6196 1393.6257 -4.38 0 0 2.3 +1Score > 16 indicates identity MAVMPEEELWK + 2 Oxidation (M)
6726 940.9276 1879.8406 1879.8258 7.90 0 0 2.9 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
2832 616.7802 1231.5458 1231.5390 5.53 0 0 1.7 +1Score > 15 indicates identity ADNDMAPLQQK + 2 Deamidated (NQ)
5850 873.3930 1744.7714 1744.7839 -7.11 1 0 2.7 +1Score > 17 indicates identity GGQYCSADYQAQLKR + Deamidated (NQ)
1674 522.2372 1042.4598 1042.4614 -1.50 0 0 0.97 +1Score > 13 indicates identity QQMEGHASR
3046 630.2864 1258.5582 1258.5621 -3.03 0 0 1.3 +1Score > 14 indicates identity ISMMPGMPSHR + Oxidation (M)
3951 711.3232 1420.6318 1420.6318 0.062 1 0 2.4 +1Score > 16 indicates identity ADDLGKGGNEESTK + Deamidated (NQ)
5967 873.3969 1744.7792 1744.7735 3.28 0 0 3.4 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
6262 900.4092 1798.8038 1798.8308 -15.0 0 0 1.7 +1Score > 15 indicates identity IYDSDSILSMHHEPR
692 441.1937 880.3728 880.3562 18.9 0 0 1.2 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
2042 549.2496 1096.4846 1096.4753 8.49 1 0 1.6 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
2178 562.7557 1123.4968 1123.4894 6.65 0 0 1.5 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
5583 846.3846 1690.7546 1690.7733 -11.0 0 0 2 +1Score > 16 indicates identity MAQEYTVEQLNHGR + Oxidation (M)
6109 886.9030 1771.7914 1771.8028 -6.42 0 0 2.2 +1Score > 16 indicates identity LNDEMFEVTFWWR
6429 913.9153 1825.8160 1825.8186 -1.39 1 0 2.7 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
371 414.1848 826.3550 826.3677 -15.3 0 0 1.1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
460 414.1881 826.3616 826.3677 -7.33 0 0 1.1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
4323 738.3355 1474.6564 1474.6582 -1.22 1 0 1.9 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
6947 954.4337 1906.8528 1906.8883 -18.6 1 0 2.8 +1Score > 17 indicates identity QPSEQELRDALFCWK + Deamidated (NQ)
4435 751.8416 1501.6686 1501.6943 -17.1 1 0 2.6 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
4687 765.3478 1528.6810 1528.6645 10.8 0 0 1.6 +1Score > 15 indicates identity MQEALMSLMQMAK + 2 Deamidated (NQ); Oxidation (M)
5142 805.8662 1609.7178 1609.7406 -14.1 1 0 1.8 +1Score > 15 indicates identity ADQINEAYERMLR + 2 Deamidated (NQ)
3157 643.7925 1285.5704 1285.5609 7.45 0 0 1.6 +1Score > 15 indicates identity SSDLFSICSDR
6536 927.4214 1852.8282 1852.8187 5.13 0 0 2.5 +1Score > 17 indicates identity GGQAEAEPDSGPTGEPAQR
3330 657.2986 1312.5826 1312.5830 -0.24 0 0 2.2 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
4799 778.8539 1555.6932 1555.7042 -7.04 0 0 1.9 +1Score > 15 indicates identity QDLTEAFQFAAEGK + 2 Deamidated (NQ)
2317 576.2618 1150.5090 1150.5288 -17.2 0 0 1.8 +1Score > 15 indicates identity QTQLTEEMR + Oxidation (M)
3646 684.3109 1366.6072 1366.6266 -14.1 1 0 2.2 +1Score > 16 indicates identity DWRETNIDYR
2642 603.2741 1204.5336 1204.5394 -4.77 0 0 2.2 +1Score > 16 indicates identity DDPSMVADLSR
6096 886.9030 1771.7914 1771.8053 -7.84 0 0 2.2 +1Score > 16 indicates identity NTFSQVWVFSDTPSR + 2 Deamidated (NQ)
4329 738.3355 1474.6564 1474.6663 -6.70 0 0 1.9 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
7056 967.9399 1933.8652 1933.8298 18.3 1 0 2.6 +1Score > 17 indicates identity QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M)
4125 724.8293 1447.6440 1447.6725 -19.7 1 0 1.2 +1Score > 13 indicates identity ECAAEITDKAGQR
4785 778.8539 1555.6932 1555.7163 -14.8 0 0 1.9 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
5122 805.8662 1609.7178 1609.7294 -7.16 0 0 1.8 +1Score > 15 indicates identity EALAMVDLEGFEDR + Oxidation (M)
2032 549.2496 1096.4846 1096.4753 8.49 1 0 1.7 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
2314 576.2618 1150.5090 1150.5117 -2.32 0 0 1.8 +1Score > 15 indicates identity YMTWDNPPK
2329 576.2618 1150.5090 1150.5117 -2.32 0 0 1.8 +1Score > 15 indicates identity YMTWDNPPK
2965 630.2864 1258.5582 1258.5621 -3.03 0 0 1.3 +1Score > 14 indicates identity ISMMPGMPSHR + Oxidation (M)
1 387.1759 772.3372
2 387.1759 772.3372
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61 387.1759 772.3372
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