MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
698 441.1939 880.3732 880.3562 19.3 0 1 1.1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
1973 549.2496 1096.4846 1096.4825 1.93 0 1 1.5 +1Score > 15 indicates identity YVTWEDQR + Deamidated (NQ)
1025 468.2127 934.4108 934.4178 -7.46 1 1 1.7 +1Score > 15 indicates identity LDEDCKR
101 387.1804 772.3462 772.3351 14.4 0 1 0.88 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
680 441.1932 880.3718 880.3562 17.7 0 1 1.1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
2767 603.2838 1204.5530 1204.5684 -12.7 0 1 3.6 +1Score > 19 indicates identity SAETLSGGEAQR
6904 954.4337 1906.8528 1906.8784 -13.4 0 1 2.5 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
1786 522.2429 1042.4712 1042.4576 13.1 0 1 1.2 +1Score > 14 indicates identity GFADAIMMR + 2 Oxidation (M)
5614 846.3846 1690.7546 1690.7403 8.49 0 1 1.9 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
6588 927.4214 1852.8282 1852.8340 -3.10 1 1 2.2 +1Score > 17 indicates identity TVNGETAERWQYDER
1846 535.7434 1069.4722 1069.4862 -13.1 0 1 0.88 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
7077 967.9399 1933.8652 1933.9017 -18.9 1 1 2.3 +1Score > 17 indicates identity QDIDRWQSTSVNDVLR + 3 Deamidated (NQ)
2050 549.2496 1096.4846 1096.4753 8.49 1 1 1.5 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
5346 819.3723 1636.7300 1636.7403 -6.24 0 1 2.2 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
2054 549.2496 1096.4846 1096.4753 8.49 1 1 1.5 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
4677 765.3478 1528.6810 1528.7005 -12.7 0 1 1.4 +1Score > 15 indicates identity EVPGVQLTNEGDNR + 2 Deamidated (NQ)
5571 846.3846 1690.7546 1690.7403 8.49 0 1 1.9 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
7052 967.9399 1933.8652 1933.8705 -2.70 1 1 2.4 +1Score > 17 indicates identity IMCAFKMMATCSNVVR + Oxidation (M)
440 414.1881 826.3616 826.3677 -7.33 0 0 1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
584 427.6943 853.3740 853.3786 -5.34 0 0 0.89 +1Score > 13 indicates identity NGTMMIR + 2 Oxidation (M)
1173 481.7188 961.4230 961.4253 -2.38 0 0 1.4 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
1052 468.2127 934.4108 934.4178 -7.46 1 0 1.7 +1Score > 15 indicates identity LDEDCKR
4779 778.8539 1555.6932 1555.7123 -12.2 1 0 1.7 +1Score > 15 indicates identity ATRAQAGDFMSLCK + Deamidated (NQ)
6123 886.9030 1771.7914 1771.7909 0.29 0 0 2 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
5277 819.3723 1636.7300 1636.7119 11.1 1 0 2.2 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
6897 954.4337 1906.8528 1906.8784 -13.4 0 0 2.6 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
5753 859.8907 1717.7668 1717.7907 -13.9 1 0 1.7 +1Score > 15 indicates identity SVVNNDPQGYPNRVR + 4 Deamidated (NQ)
5988 873.3969 1744.7792 1744.8130 -19.4 0 0 3.2 +1Score > 18 indicates identity DFQYIVNQYPGMIR + 2 Deamidated (NQ)
467 414.1881 826.3616 826.3677 -7.33 0 0 1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1053 468.2127 934.4108 934.4178 -7.47 0 0 1.7 +1Score > 15 indicates identity MNVGDVQR + Deamidated (NQ); Oxidation (M)
2478 589.7679 1177.5212 1177.5332 -10.1 1 0 1.3 +1Score > 14 indicates identity ANNPTSRMMR + Deamidated (NQ)
4438 751.8416 1501.6686 1501.6943 -17.1 1 0 2.4 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
884 454.7066 907.3986 907.3817 18.6 1 0 1.2 +1Score > 14 indicates identity ENMGRER + Deamidated (NQ); Oxidation (M)
6560 927.4214 1852.8282 1852.8625 -18.5 1 0 2.3 +1Score > 17 indicates identity NQKIGSLGMDVYENER + Deamidated (NQ)
678 441.1931 880.3716 880.3562 17.5 0 0 1.1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
5287 819.3723 1636.7300 1636.7119 11.1 1 0 2.2 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
6013 886.8923 1771.7700 1771.7683 1.00 1 0 1.5 +1Score > 15 indicates identity SGSTDQAVRDQFNSMK + 2 Deamidated (NQ)
6903 954.4337 1906.8528 1906.8784 -13.4 0 0 2.6 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
1976 549.2496 1096.4846 1096.4753 8.49 1 0 1.6 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
6732 940.9276 1879.8406 1879.8291 6.13 0 0 2.7 +1Score > 17 indicates identity MIQEQTMLNVADNSGAR + 3 Deamidated (NQ)
2660 603.2741 1204.5336 1204.5394 -4.77 0 0 2 +1Score > 16 indicates identity DDPSMVADLSR
2854 616.7802 1231.5458 1231.5680 -18.0 0 0 1.6 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
3856 697.8171 1393.6196 1393.6118 5.61 0 0 2.2 +1Score > 16 indicates identity MMLDNHALYDR + Oxidation (M)
5931 873.3969 1744.7792 1744.7904 -6.39 0 0 3.2 +1Score > 18 indicates identity DIDVDYASHSPQIER + Deamidated (NQ)
6435 913.9153 1825.8160 1825.8251 -4.96 0 0 2.6 +1Score > 17 indicates identity MTNNDTTLQLSSVLNR + 4 Deamidated (NQ); Oxidation (M)
662 441.1924 880.3702 880.3562 15.9 0 0 1.2 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
711 441.1945 880.3744 880.3596 16.9 0 0 1 +1Score > 13 indicates identity LNSQMNR + 3 Deamidated (NQ); Oxidation (M)
2198 562.7557 1123.4968 1123.4824 12.9 1 0 1.4 +1Score > 14 indicates identity RQMMGMEPK + Deamidated (NQ); Oxidation (M)
1036 468.2127 934.4108 934.4178 -7.46 1 0 1.7 +1Score > 15 indicates identity LDEDCKR
1828 535.7434 1069.4722 1069.4862 -13.1 0 0 0.92 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1906 535.7434 1069.4722 1069.4862 -13.1 1 0 0.92 +1Score > 13 indicates identity EAMGFSEKR + Oxidation (M)
1968 549.2496 1096.4846 1096.4978 -12.0 0 0 1.6 +1Score > 15 indicates identity YYGYTGAFR
2310 576.2618 1150.5090 1150.4891 17.4 0 0 1.7 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
435 414.1881 826.3616 826.3677 -7.33 0 0 1.1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1051 468.2127 934.4108 934.4178 -7.46 1 0 1.7 +1Score > 15 indicates identity LDEDCKR
1778 522.2415 1042.4684 1042.4576 10.4 0 0 1.2 +1Score > 14 indicates identity GFADAIMMR + 2 Oxidation (M)
2028 549.2496 1096.4846 1096.4753 8.49 1 0 1.6 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
2502 589.7679 1177.5212 1177.5363 -12.8 1 0 1.3 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
6615 927.4214 1852.8282 1852.8631 -18.8 0 0 2.3 +1Score > 17 indicates identity SSIQLISSNWPYWNR + 3 Deamidated (NQ)
889 454.7066 907.3986 907.3817 18.6 1 0 1.2 +1Score > 14 indicates identity RDNQAMR + 2 Deamidated (NQ); Oxidation (M)
1026 468.2127 934.4108 934.4178 -7.46 1 0 1.8 +1Score > 15 indicates identity LDEDCKR
1088 468.2127 934.4108 934.4178 -7.47 0 0 1.8 +1Score > 15 indicates identity MNVGDVQR + Deamidated (NQ); Oxidation (M)
2476 589.7679 1177.5212 1177.5013 16.9 0 0 1.3 +1Score > 14 indicates identity NDHDHAWQR
5971 873.3969 1744.7792 1744.7735 3.28 0 0 3.2 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
989 468.2127 934.4108 934.4178 -7.46 1 0 1.8 +1Score > 15 indicates identity LDEDCKR
4502 751.8416 1501.6686 1501.6909 -14.8 1 0 2.5 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
6002 873.3980 1744.7814 1744.8162 -19.9 1 0 3.2 +1Score > 18 indicates identity QCKNVQDAIADAEQR
2893 616.7802 1231.5458 1231.5680 -18.0 0 0 1.6 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
3261 657.2925 1312.5704 1312.5571 10.1 0 0 1.5 +1Score > 15 indicates identity GDVSAEYGSWNK + Deamidated (NQ)
416 414.1881 826.3616 826.3677 -7.33 0 0 1.1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1059 468.2127 934.4108 934.4178 -7.46 1 0 1.8 +1Score > 15 indicates identity LDEDCKR
1852 535.7434 1069.4722 1069.4750 -2.58 0 0 0.93 +1Score > 13 indicates identity CSTGQFLEK + Deamidated (NQ)
3345 657.2986 1312.5826 1312.5643 13.9 1 0 2.1 +1Score > 16 indicates identity HDVKGDNEENR + Deamidated (NQ)
6911 954.4337 1906.8528 1906.8731 -10.6 0 0 2.7 +1Score > 17 indicates identity LDQLTWQLMQNEQSR + 2 Deamidated (NQ); Oxidation (M)
598 427.6943 853.3740 853.3600 16.5 0 0 0.94 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
1037 468.2127 934.4108 934.4178 -7.46 1 0 1.8 +1Score > 15 indicates identity LDEDCKR
3022 630.2864 1258.5582 1258.5621 -3.03 0 0 1.3 +1Score > 14 indicates identity ISMMPGMPSHR + Oxidation (M)
3324 657.2986 1312.5826 1312.6081 -19.4 1 0 2.1 +1Score > 16 indicates identity AGGTIMFSNNKR + 2 Deamidated (NQ); Oxidation (M)
4455 751.8416 1501.6686 1501.6943 -17.1 1 0 2.5 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
522 427.6943 853.3740 853.3851 -13.0 0 0 0.94 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
2803 616.7802 1231.5458 1231.5680 -18.0 0 0 1.7 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
3148 643.7925 1285.5704 1285.5873 -13.1 0 0 1.6 +1Score > 15 indicates identity YHLDDISMHR
4461 751.8416 1501.6686 1501.6891 -13.6 1 0 2.5 +1Score > 17 indicates identity WEWSRYYAWR
3124 643.7925 1285.5704 1285.5761 -4.41 0 0 1.6 +1Score > 15 indicates identity MQAYFDQLDR
3537 670.8048 1339.5950 1339.6125 -13.0 1 0 2.3 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
4629 765.3478 1528.6810 1528.6794 1.10 0 0 1.5 +1Score > 15 indicates identity TEHTSSYYAASANK
3823 697.8171 1393.6196 1393.6217 -1.50 0 0 2.3 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
5449 832.8785 1663.7424 1663.7408 0.97 0 0 2 +1Score > 16 indicates identity GMSVEMLFDFMAVR + 2 Oxidation (M)
5616 846.3846 1690.7546 1690.7403 8.49 0 0 2 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
6801 940.9276 1879.8406 1879.8588 -9.68 0 0 2.9 +1Score > 17 indicates identity GTPLADNDDVDAFDFIR
2514 589.7679 1177.5212 1177.5013 16.9 0 0 1.4 +1Score > 14 indicates identity NDHDHAWQR
2521 589.7679 1177.5212 1177.5107 8.95 0 0 1.4 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
5291 819.3723 1636.7300 1636.7119 11.1 1 0 2.3 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
658 441.1922 880.3698 880.3562 15.4 0 0 1.2 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
5267 819.3723 1636.7300 1636.7185 7.05 0 0 2.3 +1Score > 16 indicates identity VGGLSVMCEDAEAAGR + Oxidation (M)
695 441.1938 880.3730 880.3562 19.1 0 0 1.2 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
2877 616.7802 1231.5458 1231.5680 -18.0 0 0 1.7 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
4279 738.3355 1474.6564 1474.6728 -11.1 0 0 1.9 +1Score > 15 indicates identity NQYYGITAGPAYR + 2 Deamidated (NQ)
6292 900.4092 1798.8038 1798.7870 9.35 0 0 1.7 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
6888 954.4337 1906.8528 1906.8784 -13.4 0 0 2.8 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
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