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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 7001–7100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
7006 967.9399 1933.8652
7007 967.9399 1933.8652
7008 967.9399 1933.8652
7009 967.9399 1933.8652
7010 967.9399 1933.8652
7011 967.9399 1933.8652
7012 967.9399 1933.8652
7013 967.9399 1933.8652
7014 967.9399 1933.8652
7015 967.9399 1933.8652
7016 967.9399 1933.8652
7017 967.9399 1933.8652
7018 967.9399 1933.8652
7019 967.9399 1933.8652
7020 967.9399 1933.8652
7022 967.9399 1933.8652
7023 967.9399 1933.8652
7024 967.9399 1933.8652
7025 967.9399 1933.8652
7026 967.9399 1933.8652
7027 967.9399 1933.8652
7028 967.9399 1933.8652
7029 967.9399 1933.8652
7030 967.9399 1933.8652
7031 967.9399 1933.8652
7032 967.9399 1933.8652
7033 967.9399 1933.8652
7034 967.9399 1933.8652
7035 967.9399 1933.8652
7036 967.9399 1933.8652
7037 967.9399 1933.8652
7038 967.9399 1933.8652
7039 967.9399 1933.8652
7040 967.9399 1933.8652
7041 967.9399 1933.8652
7042 967.9399 1933.8652
7043 967.9399 1933.8652
7044 967.9399 1933.8652
7045 967.9399 1933.8652
7046 967.9399 1933.8652
7047 967.9399 1933.8652
7048 967.9399 1933.8652
7049 967.9399 1933.8652
7051 967.9399 1933.8652
7053 967.9399 1933.8652
7054 967.9399 1933.8652
7055 967.9399 1933.8652
7057 967.9399 1933.8652
7058 967.9399 1933.8652
7059 967.9399 1933.8652
7061 967.9399 1933.8652
7062 967.9399 1933.8652
7063 967.9399 1933.8652
7064 967.9399 1933.8652
7065 967.9399 1933.8652
7066 967.9399 1933.8652
7067 967.9399 1933.8652
7068 967.9399 1933.8652
7069 967.9399 1933.8652
7070 967.9399 1933.8652
7071 967.9399 1933.8652
7072 967.9399 1933.8652
7073 967.9399 1933.8652
7074 967.9399 1933.8652
7075 967.9399 1933.8652
7076 967.9399 1933.8652
7078 967.9399 1933.8652
7079 967.9399 1933.8652
7081 967.9399 1933.8652
7083 967.9399 1933.8652
7085 967.9399 1933.8652
7086 967.9399 1933.8652
7087 967.9399 1933.8652
7088 967.9399 1933.8652
7089 967.9399 1933.8652
7090 967.9399 1933.8652
7091 967.9399 1933.8652
7092 967.9399 1933.8652
7093 967.9399 1933.8652
7094 967.9399 1933.8652
7095 967.9399 1933.8652
7096 967.9399 1933.8652
7097 967.9399 1933.8652
7098 967.9399 1933.8652
7099 967.9399 1933.8652
7100 967.9399 1933.8652
7101 967.9399 1933.8652
7102 967.9399 1933.8652
7103 967.9399 1933.8652
7104 967.9399 1933.8652
7105 967.9399 1933.8652
7106 967.9399 1933.8652
7107 967.9399 1933.8652
7108 967.9399 1933.8652
7109 967.9399 1933.8652
7111 967.9399 1933.8652
7112 967.9399 1933.8652
7113 967.9399 1933.8652
7114 967.9399 1933.8652
7115 967.9399 1933.8652
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