MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2184 562.7557 1123.4968 1123.5179 -18.8 0 1 1.2 +1Score > 14 indicates identity SASLSDIEMR + Oxidation (M)
3343 657.2986 1312.5826 1312.5643 13.9 1 1 1.7 +1Score > 16 indicates identity HDVKGDNEENR + Deamidated (NQ)
4260 738.3355 1474.6564 1474.6510 3.66 1 1 1.5 +1Score > 15 indicates identity QWENGKCAIPDR + 2 Deamidated (NQ)
6086 886.9030 1771.7914 1771.7571 19.4 1 1 1.8 +1Score > 16 indicates identity TRTDENGEFVMDVDK + Deamidated (NQ); Oxidation (M)
7130 967.9399 1933.8652 1933.8516 7.07 1 1 2.1 +1Score > 17 indicates identity LNSNMDADLYGYKWAR + 2 Deamidated (NQ); Oxidation (M)
1035 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
2675 603.2741 1204.5336 1204.5394 -4.75 0 1 1.7 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
3134 643.7925 1285.5704 1285.5761 -4.41 0 1 1.3 +1Score > 15 indicates identity MQAYFDQLDR
4365 738.3355 1474.6564 1474.6722 -10.7 1 1 1.5 +1Score > 15 indicates identity DQSHLSMEERVK + Deamidated (NQ); Oxidation (M)
987 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
3687 684.3109 1366.6072 1366.6153 -5.91 1 1 1.7 +1Score > 16 indicates identity FQQQKDWETR + 2 Deamidated (NQ)
5578 846.3846 1690.7546 1690.7403 8.49 0 1 1.6 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
4517 751.8416 1501.6686 1501.6620 4.44 1 1 2.1 +1Score > 17 indicates identity FDDRGDLSFMQR + Oxidation (M)
1272 481.7188 961.4230 961.4352 -12.7 0 1 1.3 +1Score > 15 indicates identity QAEELESR + Deamidated (NQ)
640 427.6943 853.3740 853.3600 16.5 0 1 0.79 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
1830 535.7434 1069.4722 1069.4862 -13.1 0 1 0.79 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2360 576.2618 1150.5090 1150.5117 -2.32 0 1 1.5 +1Score > 15 indicates identity YMTWDNPPK
3405 657.2986 1312.5826 1312.5757 5.26 0 1 1.7 +1Score > 16 indicates identity EYTWANLMER + Deamidated (NQ)
4445 751.8416 1501.6686 1501.6806 -7.95 1 1 2.1 +1Score > 17 indicates identity RLDFMMQEFNR + Oxidation (M)
432 414.1881 826.3616 826.3677 -7.33 0 1 0.91 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
6039 886.9030 1771.7914 1771.7941 -1.47 0 1 1.8 +1Score > 16 indicates identity ATLADQVNYWSNYPK + 3 Deamidated (NQ)
2047 549.2496 1096.4846 1096.4753 8.49 1 1 1.4 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
3936 711.3232 1420.6318 1420.6252 4.68 1 1 2 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
4122 724.8293 1447.6440 1447.6249 13.2 0 1 1 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
2102 549.2496 1096.4846 1096.4753 8.49 1 1 1.4 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
3585 684.3052 1366.5958 1366.6041 -6.07 0 1 1.5 +1Score > 15 indicates identity VFTDGDDTPWSK
1837 535.7434 1069.4722 1069.4862 -13.1 0 1 0.8 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
4067 711.3338 1420.6530 1420.6803 -19.1 0 1 2.8 +1Score > 18 indicates identity CALIDALMQQSR + Oxidation (M)
4436 751.8416 1501.6686 1501.6943 -17.1 1 1 2.2 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
6418 913.9153 1825.8160 1825.8186 -1.39 1 1 2.2 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
768 441.2004 880.3862 880.3926 -7.26 0 1 1.3 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
6040 886.9030 1771.7914 1771.7947 -1.85 1 1 1.8 +1Score > 16 indicates identity YRYANNDMQELEAR
3475 670.8048 1339.5950 1339.5714 17.6 0 1 1.9 +1Score > 16 indicates identity EYNTVDMSPQR + Deamidated (NQ)
6802 940.9276 1879.8406 1879.8469 -3.35 0 1 2.4 +1Score > 17 indicates identity NEVVADGNDSVTMTATVR + 2 Deamidated (NQ)
684 441.1933 880.3720 880.3562 17.9 0 1 1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
6583 927.4214 1852.8282 1852.8389 -5.74 0 1 2.1 +1Score > 17 indicates identity SLPWQAVWEMYCQR
336 414.1821 826.3496 826.3643 -17.8 0 1 0.93 +1Score > 13 indicates identity YQGMVGR + Deamidated (NQ); Oxidation (M)
3308 657.2986 1312.5826 1312.5686 10.7 1 1 1.8 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
6248 900.4092 1798.8038 1798.8270 -12.9 0 1 1.4 +1Score > 15 indicates identity VINGFMIQGGGFEPGMK + 2 Deamidated (NQ); Oxidation (M)
442 414.1881 826.3616 826.3677 -7.33 0 1 0.93 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1062 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
6912 954.4337 1906.8528 1906.8818 -15.2 0 1 2.4 +1Score > 17 indicates identity GVAMVFPGQGAQWQGMAR + Deamidated (NQ); Oxidation (M)
1027 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
4054 711.3232 1420.6318 1420.6187 9.25 1 1 2 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
2037 549.2496 1096.4846 1096.4753 8.49 1 1 1.4 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
3493 670.8048 1339.5950 1339.6045 -7.04 0 1 2 +1Score > 16 indicates identity GTVTDFPGFDER
457 414.1881 826.3616 826.3677 -7.33 0 1 0.94 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
2879 616.7802 1231.5458 1231.5680 -18.0 0 1 1.4 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
6591 927.4214 1852.8282 1852.8340 -3.10 1 1 2.1 +1Score > 17 indicates identity TVNGETAERWQYDER
1995 549.2496 1096.4846 1096.4859 -1.14 0 1 1.4 +1Score > 15 indicates identity DVGDMQLYR + Deamidated (NQ)
3392 657.2986 1312.5826 1312.5686 10.7 1 1 1.8 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
6823 954.4306 1906.8466 1906.8784 -16.7 0 1 2 +1Score > 16 indicates identity MSQFFYIHPDNPQQR
2016 549.2496 1096.4846 1096.4753 8.49 1 1 1.4 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
6725 940.9276 1879.8406 1879.8258 7.90 0 1 2.5 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
2148 562.7557 1123.4968 1123.4894 6.65 0 1 1.3 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
4819 778.8539 1555.6932 1555.7163 -14.8 0 1 1.6 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
797 441.2004 880.3862 880.3927 -7.31 0 1 1.3 +1Score > 15 indicates identity DGDGFGVSK
4500 751.8416 1501.6686 1501.6653 2.20 0 1 2.2 +1Score > 17 indicates identity IHDACVVAMSDER
1050 468.2127 934.4108 934.4178 -7.46 1 1 1.6 +1Score > 15 indicates identity LDEDCKR
3639 684.3109 1366.6072 1366.6221 -10.8 0 1 1.8 +1Score > 16 indicates identity LANAAITDMQMR + Deamidated (NQ); 2 Oxidation (M)
3792 697.8171 1393.6196 1393.6217 -1.50 0 1 2 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
438 414.1881 826.3616 826.3677 -7.33 0 1 0.95 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
2247 562.7557 1123.4968 1123.5114 -13.0 0 1 1.3 +1Score > 14 indicates identity ISGTGMCNIR + Oxidation (M)
6914 954.4337 1906.8528 1906.8877 -18.3 1 1 2.4 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
1850 535.7434 1069.4722 1069.4750 -2.58 0 1 0.83 +1Score > 13 indicates identity CSTGQFLEK + Deamidated (NQ)
2060 549.2496 1096.4846 1096.4978 -12.0 0 1 1.4 +1Score > 15 indicates identity YYGYTGAFR
6565 927.4214 1852.8282 1852.8187 5.13 0 1 2.1 +1Score > 17 indicates identity GGQAEAEPDSGPTGEPAQR
6760 940.9276 1879.8406 1879.8258 7.90 0 1 2.5 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
2687 603.2741 1204.5336 1204.5394 -4.76 1 1 1.8 +1Score > 16 indicates identity ADEPKCGATEK
6229 900.4092 1798.8038 1798.8018 1.13 1 1 1.5 +1Score > 15 indicates identity NVFMGKLNDMVNWGR + 3 Deamidated (NQ); Oxidation (M)
6886 954.4337 1906.8528 1906.8784 -13.4 0 1 2.4 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
760 441.2004 880.3862 880.3926 -7.26 0 1 1.3 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
6395 913.9153 1825.8160 1825.8451 -15.9 1 1 2.4 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
6617 927.4214 1852.8282 1852.8326 -2.36 1 1 2.1 +1Score > 17 indicates identity TLNSNQFDNEDAIKDK + 2 Deamidated (NQ)
2043 549.2496 1096.4846 1096.4753 8.49 1 1 1.4 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
2306 576.2618 1150.5090 1150.4891 17.4 0 1 1.6 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
6891 954.4337 1906.8528 1906.8784 -13.4 0 1 2.5 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
521 427.6943 853.3740 853.3851 -13.0 0 1 0.85 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
656 441.1919 880.3692 880.3562 14.8 0 1 1.1 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
4441 751.8416 1501.6686 1501.6943 -17.1 1 1 2.3 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
2026 549.2496 1096.4846 1096.4978 -12.0 0 1 1.5 +1Score > 15 indicates identity YYGYTGAFR
6580 927.4214 1852.8282 1852.8326 -2.36 1 1 2.2 +1Score > 17 indicates identity TLNSNQFDNEDAIKDK + 2 Deamidated (NQ)
87 387.1801 772.3456 772.3537 -10.5 1 1 0.86 +1Score > 13 indicates identity HKDMNK + Deamidated (NQ)
379 414.1859 826.3572 826.3677 -12.7 0 1 0.99 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1856 535.7434 1069.4722 1069.4862 -13.1 0 1 0.86 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1068 468.2127 934.4108 934.4178 -7.46 1 1 1.6 +1Score > 15 indicates identity LDEDCKR
1974 549.2496 1096.4846 1096.4753 8.49 1 1 1.5 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
372 414.1849 826.3552 826.3677 -15.1 0 1 0.95 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
784 441.2004 880.3862 880.3709 17.5 1 1 1.4 +1Score > 15 indicates identity NDMSNKR + Deamidated (NQ); Oxidation (M)
1038 468.2127 934.4108 934.4219 -11.8 0 1 1.6 +1Score > 15 indicates identity TFPDCAPK
2151 562.7557 1123.4968 1123.4894 6.65 0 1 1.3 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
2888 616.7802 1231.5458 1231.5680 -18.0 0 1 1.5 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
3510 670.8048 1339.5950 1339.6078 -9.51 0 1 2.1 +1Score > 16 indicates identity EELSAFLSQCR + Deamidated (NQ)
1106 468.2127 934.4108 934.4178 -7.46 1 1 1.6 +1Score > 15 indicates identity LDEDCKR
1182 481.7188 961.4230 961.4287 -5.91 0 1 1.4 +1Score > 15 indicates identity MTDLSHSR + Oxidation (M)
2959 630.2864 1258.5582 1258.5612 -2.31 1 1 1.2 +1Score > 14 indicates identity QQNAQKMHEK + 2 Deamidated (NQ); Oxidation (M)
3971 711.3232 1420.6318 1420.6187 9.25 1 1 2.1 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
549 427.6943 853.3740 853.3851 -13.0 0 1 0.87 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
4437 751.8416 1501.6686 1501.6943 -17.1 1 1 2.3 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
446 414.1881 826.3616 826.3677 -7.33 0 1 1 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
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