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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6801–6900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6762 940.9276 1879.8406
6763 940.9276 1879.8406
6764 940.9276 1879.8406
6765 940.9276 1879.8406
6766 940.9276 1879.8406
6768 940.9276 1879.8406
6769 940.9276 1879.8406
6770 940.9276 1879.8406
6771 940.9276 1879.8406
6772 940.9276 1879.8406
6773 940.9276 1879.8406
6774 940.9276 1879.8406
6775 940.9276 1879.8406
6776 940.9276 1879.8406
6777 940.9276 1879.8406
6778 940.9276 1879.8406
6779 940.9276 1879.8406
6780 940.9276 1879.8406
6781 940.9276 1879.8406
6782 940.9276 1879.8406
6783 940.9276 1879.8406
6784 940.9276 1879.8406
6785 940.9276 1879.8406
6786 940.9276 1879.8406
6787 940.9276 1879.8406
6788 940.9276 1879.8406
6789 940.9276 1879.8406
6790 940.9276 1879.8406
6791 940.9276 1879.8406
6792 940.9276 1879.8406
6793 940.9276 1879.8406
6794 940.9276 1879.8406
6795 940.9276 1879.8406
6796 940.9276 1879.8406
6797 940.9276 1879.8406
6798 940.9276 1879.8406
6799 940.9276 1879.8406
6800 940.9276 1879.8406
6803 940.9276 1879.8406
6804 940.9276 1879.8406
6805 940.9276 1879.8406
6806 940.9276 1879.8406
6807 940.9276 1879.8406
6808 940.9276 1879.8406
6809 940.9276 1879.8406
6810 940.9276 1879.8406
6811 940.9276 1879.8406
6812 940.9276 1879.8406
6813 940.9276 1879.8406
6814 940.9276 1879.8406
6815 940.9276 1879.8406
6816 940.9276 1879.8406
6817 940.9276 1879.8406
6818 940.9276 1879.8406
6819 940.9276 1879.8406
6820 940.9276 1879.8406
6821 940.9276 1879.8406
6822 954.4302 1906.8458
6824 954.4310 1906.8474
6825 954.4326 1906.8506
6826 954.4329 1906.8512
6827 954.4334 1906.8522
6828 954.4337 1906.8528
6829 954.4337 1906.8528
6830 954.4337 1906.8528
6831 954.4337 1906.8528
6832 954.4337 1906.8528
6833 954.4337 1906.8528
6834 954.4337 1906.8528
6835 954.4337 1906.8528
6836 954.4337 1906.8528
6837 954.4337 1906.8528
6838 954.4337 1906.8528
6839 954.4337 1906.8528
6840 954.4337 1906.8528
6841 954.4337 1906.8528
6842 954.4337 1906.8528
6843 954.4337 1906.8528
6844 954.4337 1906.8528
6845 954.4337 1906.8528
6846 954.4337 1906.8528
6847 954.4337 1906.8528
6848 954.4337 1906.8528
6850 954.4337 1906.8528
6853 954.4337 1906.8528
6855 954.4337 1906.8528
6856 954.4337 1906.8528
6857 954.4337 1906.8528
6858 954.4337 1906.8528
6860 954.4337 1906.8528
6862 954.4337 1906.8528
6863 954.4337 1906.8528
6865 954.4337 1906.8528
6869 954.4337 1906.8528
6870 954.4337 1906.8528
6871 954.4337 1906.8528
6877 954.4337 1906.8528
6878 954.4337 1906.8528
6879 954.4337 1906.8528
6880 954.4337 1906.8528
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