MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6701–6800 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6646 927.4214 1852.8282
6647 927.4214 1852.8282
6648 927.4214 1852.8282
6649 927.4214 1852.8282
6650 927.4214 1852.8282
6651 927.4214 1852.8282
6652 927.4214 1852.8282
6653 927.4214 1852.8282
6654 927.4233 1852.8320
6655 927.4270 1852.8394
6657 927.4346 1852.8546
6658 927.4382 1852.8618
6659 927.4384 1852.8622
6660 940.9276 1879.8406
6661 940.9276 1879.8406
6662 940.9276 1879.8406
6663 940.9276 1879.8406
6664 940.9276 1879.8406
6665 940.9276 1879.8406
6666 940.9276 1879.8406
6667 940.9276 1879.8406
6668 940.9276 1879.8406
6669 940.9276 1879.8406
6670 940.9276 1879.8406
6671 940.9276 1879.8406
6672 940.9276 1879.8406
6673 940.9276 1879.8406
6674 940.9276 1879.8406
6675 940.9276 1879.8406
6676 940.9276 1879.8406
6677 940.9276 1879.8406
6678 940.9276 1879.8406
6679 940.9276 1879.8406
6680 940.9276 1879.8406
6681 940.9276 1879.8406
6682 940.9276 1879.8406
6683 940.9276 1879.8406
6684 940.9276 1879.8406
6685 940.9276 1879.8406
6686 940.9276 1879.8406
6687 940.9276 1879.8406
6688 940.9276 1879.8406
6689 940.9276 1879.8406
6690 940.9276 1879.8406
6691 940.9276 1879.8406
6692 940.9276 1879.8406
6693 940.9276 1879.8406
6694 940.9276 1879.8406
6695 940.9276 1879.8406
6696 940.9276 1879.8406
6697 940.9276 1879.8406
6698 940.9276 1879.8406
6700 940.9276 1879.8406
6701 940.9276 1879.8406
6702 940.9276 1879.8406
6703 940.9276 1879.8406
6704 940.9276 1879.8406
6705 940.9276 1879.8406
6706 940.9276 1879.8406
6707 940.9276 1879.8406
6708 940.9276 1879.8406
6710 940.9276 1879.8406
6711 940.9276 1879.8406
6712 940.9276 1879.8406
6713 940.9276 1879.8406
6714 940.9276 1879.8406
6715 940.9276 1879.8406
6716 940.9276 1879.8406
6718 940.9276 1879.8406
6719 940.9276 1879.8406
6720 940.9276 1879.8406
6722 940.9276 1879.8406
6723 940.9276 1879.8406
6724 940.9276 1879.8406
6727 940.9276 1879.8406
6728 940.9276 1879.8406
6730 940.9276 1879.8406
6731 940.9276 1879.8406
6733 940.9276 1879.8406
6734 940.9276 1879.8406
6735 940.9276 1879.8406
6738 940.9276 1879.8406
6739 940.9276 1879.8406
6740 940.9276 1879.8406
6741 940.9276 1879.8406
6742 940.9276 1879.8406
6744 940.9276 1879.8406
6745 940.9276 1879.8406
6746 940.9276 1879.8406
6747 940.9276 1879.8406
6748 940.9276 1879.8406
6749 940.9276 1879.8406
6750 940.9276 1879.8406
6751 940.9276 1879.8406
6752 940.9276 1879.8406
6753 940.9276 1879.8406
6754 940.9276 1879.8406
6755 940.9276 1879.8406
6758 940.9276 1879.8406
6759 940.9276 1879.8406
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