MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6501–6600 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6405 913.9153 1825.8160
6406 913.9153 1825.8160
6407 913.9153 1825.8160
6408 913.9153 1825.8160
6409 913.9153 1825.8160
6411 913.9153 1825.8160
6412 913.9153 1825.8160
6413 913.9153 1825.8160
6414 913.9153 1825.8160
6415 913.9153 1825.8160
6417 913.9153 1825.8160
6419 913.9153 1825.8160
6420 913.9153 1825.8160
6421 913.9153 1825.8160
6422 913.9153 1825.8160
6423 913.9153 1825.8160
6424 913.9153 1825.8160
6425 913.9153 1825.8160
6427 913.9153 1825.8160
6428 913.9153 1825.8160
6430 913.9153 1825.8160
6431 913.9153 1825.8160
6432 913.9153 1825.8160
6433 913.9153 1825.8160
6436 913.9153 1825.8160
6439 913.9153 1825.8160
6440 913.9153 1825.8160
6441 913.9153 1825.8160
6442 913.9153 1825.8160
6443 913.9153 1825.8160
6444 913.9153 1825.8160
6445 913.9153 1825.8160
6446 913.9153 1825.8160
6447 913.9153 1825.8160
6448 913.9153 1825.8160
6449 913.9153 1825.8160
6450 913.9153 1825.8160
6451 913.9153 1825.8160
6453 913.9153 1825.8160
6454 913.9153 1825.8160
6455 913.9153 1825.8160
6456 913.9153 1825.8160
6457 913.9153 1825.8160
6458 913.9153 1825.8160
6459 913.9153 1825.8160
6460 913.9153 1825.8160
6461 913.9153 1825.8160
6462 913.9153 1825.8160
6463 913.9153 1825.8160
6464 913.9153 1825.8160
6465 913.9153 1825.8160
6466 913.9153 1825.8160
6467 913.9153 1825.8160
6468 913.9153 1825.8160
6469 913.9153 1825.8160
6470 913.9153 1825.8160
6471 913.9153 1825.8160
6472 913.9153 1825.8160
6474 913.9153 1825.8160
6475 913.9153 1825.8160
6476 913.9153 1825.8160
6477 913.9153 1825.8160
6478 913.9153 1825.8160
6479 913.9153 1825.8160
6480 913.9153 1825.8160
6481 913.9153 1825.8160
6482 913.9153 1825.8160
6483 913.9153 1825.8160
6484 913.9153 1825.8160
6485 913.9153 1825.8160
6486 913.9153 1825.8160
6488 913.9153 1825.8160
6489 913.9153 1825.8160
6490 913.9153 1825.8160
6491 913.9153 1825.8160
6492 913.9153 1825.8160
6493 913.9153 1825.8160
6494 913.9153 1825.8160
6495 913.9153 1825.8160
6496 913.9153 1825.8160
6497 913.9153 1825.8160
6498 927.4214 1852.8282
6499 927.4214 1852.8282
6500 927.4214 1852.8282
6501 927.4214 1852.8282
6502 927.4214 1852.8282
6503 927.4214 1852.8282
6504 927.4214 1852.8282
6505 927.4214 1852.8282
6506 927.4214 1852.8282
6507 927.4214 1852.8282
6508 927.4214 1852.8282
6509 927.4214 1852.8282
6510 927.4214 1852.8282
6511 927.4214 1852.8282
6512 927.4214 1852.8282
6513 927.4214 1852.8282
6514 927.4214 1852.8282
6515 927.4214 1852.8282
6516 927.4214 1852.8282
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