MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6401–6500 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6287 900.4092 1798.8038
6288 900.4092 1798.8038
6289 900.4092 1798.8038
6290 900.4092 1798.8038
6291 900.4092 1798.8038
6293 900.4092 1798.8038
6294 900.4092 1798.8038
6296 900.4092 1798.8038
6297 900.4092 1798.8038
6298 900.4092 1798.8038
6300 900.4092 1798.8038
6301 900.4092 1798.8038
6302 900.4092 1798.8038
6303 900.4092 1798.8038
6304 900.4092 1798.8038
6305 900.4092 1798.8038
6306 900.4092 1798.8038
6307 900.4092 1798.8038
6310 900.4092 1798.8038
6311 900.4092 1798.8038
6313 900.4092 1798.8038
6314 900.4092 1798.8038
6315 900.4092 1798.8038
6316 900.4092 1798.8038
6317 900.4092 1798.8038
6318 900.4092 1798.8038
6319 900.4092 1798.8038
6322 900.4092 1798.8038
6323 900.4092 1798.8038
6325 900.4092 1798.8038
6327 900.4092 1798.8038
6328 900.4092 1798.8038
6329 900.4092 1798.8038
6333 900.4092 1798.8038
6334 900.4094 1798.8042
6337 913.9006 1825.7866
6338 913.9022 1825.7898
6339 913.9153 1825.8160
6340 913.9153 1825.8160
6341 913.9153 1825.8160
6342 913.9153 1825.8160
6343 913.9153 1825.8160
6344 913.9153 1825.8160
6345 913.9153 1825.8160
6346 913.9153 1825.8160
6347 913.9153 1825.8160
6348 913.9153 1825.8160
6349 913.9153 1825.8160
6350 913.9153 1825.8160
6351 913.9153 1825.8160
6352 913.9153 1825.8160
6353 913.9153 1825.8160
6354 913.9153 1825.8160
6355 913.9153 1825.8160
6356 913.9153 1825.8160
6357 913.9153 1825.8160
6358 913.9153 1825.8160
6359 913.9153 1825.8160
6360 913.9153 1825.8160
6361 913.9153 1825.8160
6362 913.9153 1825.8160
6363 913.9153 1825.8160
6364 913.9153 1825.8160
6365 913.9153 1825.8160
6366 913.9153 1825.8160
6367 913.9153 1825.8160
6368 913.9153 1825.8160
6369 913.9153 1825.8160
6370 913.9153 1825.8160
6371 913.9153 1825.8160
6372 913.9153 1825.8160
6373 913.9153 1825.8160
6374 913.9153 1825.8160
6375 913.9153 1825.8160
6376 913.9153 1825.8160
6377 913.9153 1825.8160
6378 913.9153 1825.8160
6379 913.9153 1825.8160
6380 913.9153 1825.8160
6381 913.9153 1825.8160
6382 913.9153 1825.8160
6383 913.9153 1825.8160
6384 913.9153 1825.8160
6385 913.9153 1825.8160
6386 913.9153 1825.8160
6387 913.9153 1825.8160
6388 913.9153 1825.8160
6389 913.9153 1825.8160
6390 913.9153 1825.8160
6391 913.9153 1825.8160
6392 913.9153 1825.8160
6393 913.9153 1825.8160
6394 913.9153 1825.8160
6396 913.9153 1825.8160
6397 913.9153 1825.8160
6398 913.9153 1825.8160
6399 913.9153 1825.8160
6400 913.9153 1825.8160
6401 913.9153 1825.8160
6402 913.9153 1825.8160
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