MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6301–6400 (out of 7290)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6172 886.9102 1771.8058
6173 886.9174 1771.8202
6174 900.4092 1798.8038
6175 900.4092 1798.8038
6176 900.4092 1798.8038
6177 900.4092 1798.8038
6178 900.4092 1798.8038
6179 900.4092 1798.8038
6180 900.4092 1798.8038
6181 900.4092 1798.8038
6182 900.4092 1798.8038
6183 900.4092 1798.8038
6184 900.4092 1798.8038
6185 900.4092 1798.8038
6186 900.4092 1798.8038
6187 900.4092 1798.8038
6188 900.4092 1798.8038
6189 900.4092 1798.8038
6190 900.4092 1798.8038
6191 900.4092 1798.8038
6192 900.4092 1798.8038
6193 900.4092 1798.8038
6194 900.4092 1798.8038
6195 900.4092 1798.8038
6197 900.4092 1798.8038
6198 900.4092 1798.8038
6199 900.4092 1798.8038
6200 900.4092 1798.8038
6201 900.4092 1798.8038
6202 900.4092 1798.8038
6203 900.4092 1798.8038
6204 900.4092 1798.8038
6205 900.4092 1798.8038
6206 900.4092 1798.8038
6207 900.4092 1798.8038
6208 900.4092 1798.8038
6209 900.4092 1798.8038
6210 900.4092 1798.8038
6211 900.4092 1798.8038
6213 900.4092 1798.8038
6214 900.4092 1798.8038
6215 900.4092 1798.8038
6216 900.4092 1798.8038
6217 900.4092 1798.8038
6218 900.4092 1798.8038
6219 900.4092 1798.8038
6220 900.4092 1798.8038
6221 900.4092 1798.8038
6222 900.4092 1798.8038
6223 900.4092 1798.8038
6224 900.4092 1798.8038
6225 900.4092 1798.8038
6226 900.4092 1798.8038
6228 900.4092 1798.8038
6230 900.4092 1798.8038
6231 900.4092 1798.8038
6233 900.4092 1798.8038
6234 900.4092 1798.8038
6235 900.4092 1798.8038
6236 900.4092 1798.8038
6237 900.4092 1798.8038
6238 900.4092 1798.8038
6239 900.4092 1798.8038
6240 900.4092 1798.8038
6241 900.4092 1798.8038
6242 900.4092 1798.8038
6243 900.4092 1798.8038
6244 900.4092 1798.8038
6245 900.4092 1798.8038
6246 900.4092 1798.8038
6247 900.4092 1798.8038
6249 900.4092 1798.8038
6250 900.4092 1798.8038
6251 900.4092 1798.8038
6252 900.4092 1798.8038
6253 900.4092 1798.8038
6254 900.4092 1798.8038
6255 900.4092 1798.8038
6257 900.4092 1798.8038
6260 900.4092 1798.8038
6261 900.4092 1798.8038
6264 900.4092 1798.8038
6265 900.4092 1798.8038
6267 900.4092 1798.8038
6268 900.4092 1798.8038
6269 900.4092 1798.8038
6270 900.4092 1798.8038
6271 900.4092 1798.8038
6272 900.4092 1798.8038
6273 900.4092 1798.8038
6274 900.4092 1798.8038
6275 900.4092 1798.8038
6276 900.4092 1798.8038
6278 900.4092 1798.8038
6280 900.4092 1798.8038
6281 900.4092 1798.8038
6282 900.4092 1798.8038
6283 900.4092 1798.8038
6284 900.4092 1798.8038
6285 900.4092 1798.8038
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