MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6101–6200 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5943 873.3969 1744.7792
5944 873.3969 1744.7792
5946 873.3969 1744.7792
5948 873.3969 1744.7792
5949 873.3969 1744.7792
5950 873.3969 1744.7792
5951 873.3969 1744.7792
5952 873.3969 1744.7792
5953 873.3969 1744.7792
5954 873.3969 1744.7792
5955 873.3969 1744.7792
5956 873.3969 1744.7792
5958 873.3969 1744.7792
5959 873.3969 1744.7792
5961 873.3969 1744.7792
5962 873.3969 1744.7792
5963 873.3969 1744.7792
5964 873.3969 1744.7792
5965 873.3969 1744.7792
5966 873.3969 1744.7792
5968 873.3969 1744.7792
5969 873.3969 1744.7792
5970 873.3969 1744.7792
5972 873.3969 1744.7792
5974 873.3969 1744.7792
5975 873.3969 1744.7792
5976 873.3969 1744.7792
5977 873.3969 1744.7792
5978 873.3969 1744.7792
5979 873.3969 1744.7792
5980 873.3969 1744.7792
5981 873.3969 1744.7792
5982 873.3969 1744.7792
5983 873.3969 1744.7792
5984 873.3969 1744.7792
5986 873.3969 1744.7792
5989 873.3969 1744.7792
5990 873.3969 1744.7792
5991 873.3969 1744.7792
5992 873.3969 1744.7792
5993 873.3969 1744.7792
5994 873.3969 1744.7792
5995 873.3969 1744.7792
5996 873.3969 1744.7792
5997 873.3969 1744.7792
5998 873.3969 1744.7792
5999 873.3969 1744.7792
6000 873.3969 1744.7792
6001 873.3980 1744.7814
6004 873.4017 1744.7888
6006 873.4052 1744.7958
6007 873.4058 1744.7970
6008 873.4059 1744.7972
6009 873.4071 1744.7996
6010 873.4099 1744.8052
6011 873.4110 1744.8074
6012 886.8906 1771.7666
6014 886.8934 1771.7722
6015 886.8950 1771.7754
6016 886.9007 1771.7868
6017 886.9028 1771.7910
6018 886.9030 1771.7914
6019 886.9030 1771.7914
6020 886.9030 1771.7914
6021 886.9030 1771.7914
6022 886.9030 1771.7914
6023 886.9030 1771.7914
6024 886.9030 1771.7914
6025 886.9030 1771.7914
6026 886.9030 1771.7914
6027 886.9030 1771.7914
6028 886.9030 1771.7914
6029 886.9030 1771.7914
6030 886.9030 1771.7914
6031 886.9030 1771.7914
6032 886.9030 1771.7914
6033 886.9030 1771.7914
6034 886.9030 1771.7914
6035 886.9030 1771.7914
6037 886.9030 1771.7914
6038 886.9030 1771.7914
6041 886.9030 1771.7914
6042 886.9030 1771.7914
6043 886.9030 1771.7914
6044 886.9030 1771.7914
6045 886.9030 1771.7914
6046 886.9030 1771.7914
6047 886.9030 1771.7914
6048 886.9030 1771.7914
6049 886.9030 1771.7914
6050 886.9030 1771.7914
6051 886.9030 1771.7914
6052 886.9030 1771.7914
6053 886.9030 1771.7914
6054 886.9030 1771.7914
6055 886.9030 1771.7914
6056 886.9030 1771.7914
6057 886.9030 1771.7914
6058 886.9030 1771.7914
6059 886.9030 1771.7914
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