MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6001–6100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5828 859.8907 1717.7668
5829 859.8907 1717.7668
5830 859.8907 1717.7668
5831 859.8907 1717.7668
5832 859.8907 1717.7668
5833 859.8907 1717.7668
5835 859.8907 1717.7668
5836 859.8907 1717.7668
5837 859.8907 1717.7668
5838 859.8907 1717.7668
5839 859.8907 1717.7668
5840 859.8907 1717.7668
5841 859.8907 1717.7668
5842 859.8907 1717.7668
5843 859.8907 1717.7668
5844 859.8907 1717.7668
5845 859.8907 1717.7668
5846 859.8907 1717.7668
5847 859.8907 1717.7668
5848 859.8907 1717.7668
5849 859.8907 1717.7668
5851 873.3949 1744.7752
5852 873.3953 1744.7760
5854 873.3961 1744.7776
5855 873.3969 1744.7792
5856 873.3969 1744.7792
5857 873.3969 1744.7792
5858 873.3969 1744.7792
5859 873.3969 1744.7792
5860 873.3969 1744.7792
5861 873.3969 1744.7792
5862 873.3969 1744.7792
5863 873.3969 1744.7792
5864 873.3969 1744.7792
5865 873.3969 1744.7792
5866 873.3969 1744.7792
5867 873.3969 1744.7792
5868 873.3969 1744.7792
5870 873.3969 1744.7792
5871 873.3969 1744.7792
5872 873.3969 1744.7792
5874 873.3969 1744.7792
5875 873.3969 1744.7792
5876 873.3969 1744.7792
5878 873.3969 1744.7792
5879 873.3969 1744.7792
5880 873.3969 1744.7792
5881 873.3969 1744.7792
5882 873.3969 1744.7792
5883 873.3969 1744.7792
5884 873.3969 1744.7792
5885 873.3969 1744.7792
5886 873.3969 1744.7792
5887 873.3969 1744.7792
5888 873.3969 1744.7792
5889 873.3969 1744.7792
5890 873.3969 1744.7792
5891 873.3969 1744.7792
5892 873.3969 1744.7792
5893 873.3969 1744.7792
5894 873.3969 1744.7792
5895 873.3969 1744.7792
5896 873.3969 1744.7792
5897 873.3969 1744.7792
5898 873.3969 1744.7792
5900 873.3969 1744.7792
5901 873.3969 1744.7792
5902 873.3969 1744.7792
5903 873.3969 1744.7792
5904 873.3969 1744.7792
5906 873.3969 1744.7792
5907 873.3969 1744.7792
5908 873.3969 1744.7792
5909 873.3969 1744.7792
5912 873.3969 1744.7792
5913 873.3969 1744.7792
5914 873.3969 1744.7792
5915 873.3969 1744.7792
5917 873.3969 1744.7792
5918 873.3969 1744.7792
5919 873.3969 1744.7792
5920 873.3969 1744.7792
5921 873.3969 1744.7792
5922 873.3969 1744.7792
5923 873.3969 1744.7792
5924 873.3969 1744.7792
5925 873.3969 1744.7792
5926 873.3969 1744.7792
5928 873.3969 1744.7792
5929 873.3969 1744.7792
5930 873.3969 1744.7792
5932 873.3969 1744.7792
5934 873.3969 1744.7792
5936 873.3969 1744.7792
5937 873.3969 1744.7792
5938 873.3969 1744.7792
5939 873.3969 1744.7792
5940 873.3969 1744.7792
5941 873.3969 1744.7792
5942 873.3969 1744.7792
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