MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3168 643.7925 1285.5704 1285.5761 -4.41 0 2 1.1 +1Score > 15 indicates identity MQAYFDQLDR
3316 657.2986 1312.5826 1312.5830 -0.24 0 2 1.5 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
5873 873.3969 1744.7792 1744.7648 8.31 1 2 2.4 +1Score > 18 indicates identity EEEKAFLMECASTGK + Oxidation (M)
1049 468.2127 934.4108 934.4178 -7.46 1 2 1.3 +1Score > 15 indicates identity LDEDCKR
425 414.1881 826.3616 826.3677 -7.33 0 2 0.79 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1682 522.2372 1042.4598 1042.4614 -1.50 0 2 0.69 +1Score > 13 indicates identity QQMEGHASR
2025 549.2496 1096.4846 1096.4753 8.49 1 2 1.2 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
2533 589.7679 1177.5212 1177.5298 -7.29 0 2 1 +1Score > 14 indicates identity MPQHDQLHR + Deamidated (NQ); Oxidation (M)
4012 711.3232 1420.6318 1420.6331 -0.88 0 2 1.7 +1Score > 16 indicates identity HNGGQVIHSADER + 2 Deamidated (NQ)
5286 819.3723 1636.7300 1636.7119 11.1 1 2 1.7 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
6743 940.9276 1879.8406 1879.8557 -7.98 0 2 2.1 +1Score > 17 indicates identity SCAFLIADGVMPSNENR
1821 535.7434 1069.4722 1069.4862 -13.1 0 2 0.7 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2045 549.2496 1096.4846 1096.4819 2.52 1 2 1.2 +1Score > 15 indicates identity MSGGETDTRK + Oxidation (M)
4218 724.8293 1447.6440 1447.6476 -2.42 0 2 0.87 +1Score > 13 indicates identity FCQALMTELYR + Deamidated (NQ); Oxidation (M)
436 414.1881 826.3616 826.3677 -7.33 0 2 0.8 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
6295 900.4092 1798.8038 1798.7870 9.35 0 2 1.2 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
2092 549.2496 1096.4846 1096.4819 2.52 1 2 1.2 +1Score > 15 indicates identity MSGGETDTRK + Oxidation (M)
4111 724.8293 1447.6440 1447.6249 13.2 0 2 0.88 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
4177 724.8293 1447.6440 1447.6289 10.4 0 2 0.88 +1Score > 13 indicates identity MTYAVEFDNVSR + Deamidated (NQ); Oxidation (M)
6473 913.9153 1825.8160 1825.8482 -17.6 1 2 2 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
1089 468.2127 934.4108 934.4178 -7.47 0 2 1.3 +1Score > 15 indicates identity MNVGDVQR + Deamidated (NQ); Oxidation (M)
2275 562.7557 1123.4968 1123.5002 -2.96 1 2 1.1 +1Score > 14 indicates identity EAMADRVVMA + 2 Oxidation (M)
4444 751.8416 1501.6686 1501.6943 -17.1 1 2 1.9 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
5247 819.3723 1636.7300 1636.7185 7.05 0 2 1.7 +1Score > 16 indicates identity VGGLSVMCEDAEAAGR + Oxidation (M)
5985 873.3969 1744.7792 1744.7735 3.28 0 2 2.5 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
6889 954.4337 1906.8528 1906.8784 -13.4 0 2 2 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
1034 468.2127 934.4108 934.4178 -7.46 1 2 1.3 +1Score > 15 indicates identity LDEDCKR
5622 846.3846 1690.7546 1690.7872 -19.2 0 1 1.5 +1Score > 16 indicates identity HEDMYTAINELINK + Deamidated (NQ)
1279 481.7188 961.4230 961.4352 -12.7 0 1 1.1 +1Score > 15 indicates identity VNDQEISR + 2 Deamidated (NQ)
2283 576.2596 1150.5046 1150.5254 -18.1 1 1 1.6 +1Score > 16 indicates identity LEFNNDNRK + 2 Deamidated (NQ)
4522 751.8416 1501.6686 1501.6909 -14.8 1 1 1.9 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
6256 900.4092 1798.8038 1798.8308 -15.0 0 1 1.2 +1Score > 15 indicates identity IYDSDSILSMHHEPR
657 441.1920 880.3694 880.3562 15.0 0 1 0.93 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
2165 562.7557 1123.4968 1123.5067 -8.75 0 1 1.1 +1Score > 14 indicates identity QQETAVATMK + 2 Deamidated (NQ); Oxidation (M)
2323 576.2618 1150.5090 1150.5117 -2.32 0 1 1.3 +1Score > 15 indicates identity YMTWDNPPK
6036 886.9030 1771.7914 1771.8087 -9.73 0 1 1.6 +1Score > 16 indicates identity NTFNDYLDACDALLK
756 441.2004 880.3862 880.3862 0.11 0 1 1.1 +1Score > 15 indicates identity GFCAGVDR
1794 535.7333 1069.4520 1069.4386 12.6 0 1 0.72 +1Score > 13 indicates identity ETCFAEEGK
5021 792.3600 1582.7054 1582.7045 0.58 1 1 1.2 +1Score > 15 indicates identity CQYETLVENNRR + 2 Deamidated (NQ)
6881 954.4337 1906.8528 1906.8784 -13.4 0 1 2.1 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
2029 549.2496 1096.4846 1096.4753 8.49 1 1 1.2 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
6308 900.4092 1798.8038 1798.7870 9.35 0 1 1.3 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
4013 711.3232 1420.6318 1420.6331 -0.88 0 1 1.8 +1Score > 16 indicates identity HNGGQVIHSADER + 2 Deamidated (NQ)
4477 751.8416 1501.6686 1501.6772 -5.69 0 1 2 +1Score > 17 indicates identity YHQWMNELPER
6545 927.4214 1852.8282 1852.8625 -18.5 1 1 1.8 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
776 441.2004 880.3862 880.3926 -7.26 0 1 1.2 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
2852 616.7802 1231.5458 1231.5680 -18.0 0 1 1.3 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
6616 927.4214 1852.8282 1852.8631 -18.8 0 1 1.9 +1Score > 17 indicates identity SSIQLISSNWPYWNR + 3 Deamidated (NQ)
5362 819.3874 1636.7602 1636.7805 -12.4 1 1 3 +1Score > 19 indicates identity RYASAASDVDAQINR + Deamidated (NQ)
7082 967.9399 1933.8652 1933.8767 -5.93 1 1 1.9 +1Score > 17 indicates identity MLQLNENKQFAFFQR + 5 Deamidated (NQ); Oxidation (M)
5754 859.8907 1717.7668 1717.7577 5.32 0 1 1.4 +1Score > 15 indicates identity GAQLASDDNMLPDSQR + Deamidated (NQ)
1095 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
6114 886.9030 1771.7914 1771.7909 0.29 0 1 1.7 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
6905 954.4337 1906.8528 1906.8877 -18.3 1 1 2.1 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
1687 522.2372 1042.4598 1042.4432 16.0 1 1 0.74 +1Score > 13 indicates identity MMMMQSRK + Deamidated (NQ)
2762 603.2802 1204.5458 1204.5684 -18.7 0 1 2.2 +1Score > 17 indicates identity SAETLSGGEAQR
4174 724.8293 1447.6440 1447.6476 -2.42 0 1 0.92 +1Score > 13 indicates identity FCQALMTELYR + Deamidated (NQ); Oxidation (M)
4686 765.3478 1528.6810 1528.6688 8.00 1 1 1.2 +1Score > 15 indicates identity VTAGNHCDNLRNR + 3 Deamidated (NQ)
5973 873.3969 1744.7792 1744.7735 3.28 0 1 2.6 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
1677 522.2372 1042.4598 1042.4432 16.0 1 1 0.74 +1Score > 13 indicates identity MMMMQSRK + Deamidated (NQ)
2807 616.7802 1231.5458 1231.5615 -12.7 1 1 1.3 +1Score > 15 indicates identity IREMSDNDPR
2810 616.7802 1231.5458 1231.5615 -12.7 1 1 1.3 +1Score > 15 indicates identity IREMSDNDPR
5441 832.8785 1663.7424 1663.7723 -17.9 1 1 1.6 +1Score > 16 indicates identity TNGLMAQAQQQLGKR + 5 Deamidated (NQ); Oxidation (M)
1042 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
2369 576.2618 1150.5090 1150.5111 -1.76 0 1 1.4 +1Score > 15 indicates identity LMGMTPTGNGR + Deamidated (NQ); Oxidation (M)
3662 684.3109 1366.6072 1366.6299 -16.6 1 1 1.6 +1Score > 16 indicates identity SRDAFIDEMQR
179 400.6820 799.3494 799.3647 -19.0 0 1 0.75 +1Score > 13 indicates identity INCGAHK + Deamidated (NQ)
6170 886.9030 1771.7914 1771.7968 -3.04 0 1 1.7 +1Score > 16 indicates identity SQSTVGAGDSMVGAMTLK + Deamidated (NQ); 2 Oxidation (M)
2041 549.2496 1096.4846 1096.4753 8.49 1 1 1.3 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
3346 657.2986 1312.5826 1312.6081 -19.4 1 1 1.6 +1Score > 16 indicates identity AGGTIMFSNNKR + 2 Deamidated (NQ); Oxidation (M)
1832 535.7434 1069.4722 1069.4862 -13.1 0 1 0.75 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2052 549.2496 1096.4846 1096.4978 -12.0 0 1 1.3 +1Score > 15 indicates identity YYGYTGAFR
6890 954.4337 1906.8528 1906.8618 -4.70 0 1 2.2 +1Score > 17 indicates identity SNIINLEWQQIDMQR + 4 Deamidated (NQ); Oxidation (M)
410 414.1881 826.3616 826.3677 -7.33 0 1 0.87 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
2150 562.7557 1123.4968 1123.4968 0.046 0 1 1.2 +1Score > 14 indicates identity HADTAMYTAK + Oxidation (M)
2508 589.7679 1177.5212 1177.5363 -12.8 1 1 1.1 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
4434 751.8416 1501.6686 1501.6943 -17.1 1 1 2 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
1043 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
4023 711.3232 1420.6318 1420.6252 4.68 1 1 1.9 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
742 441.2004 880.3862 880.3709 17.5 0 1 1.2 +1Score > 15 indicates identity TQNMGGTR + Deamidated (NQ); Oxidation (M)
2172 562.7557 1123.4968 1123.4968 0.046 0 1 1.2 +1Score > 14 indicates identity HADTAMYTAK + Oxidation (M)
4859 778.8539 1555.6932 1555.7123 -12.2 1 1 1.5 +1Score > 15 indicates identity ATRAQAGDFMSLCK + Deamidated (NQ)
5591 846.3846 1690.7546 1690.7403 8.49 0 1 1.6 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
102 387.1804 772.3462 772.3351 14.4 0 1 0.77 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
1939 535.7434 1069.4722 1069.4862 -13.1 0 1 0.77 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2384 576.2618 1150.5090 1150.5117 -2.32 0 1 1.4 +1Score > 15 indicates identity YMTWDNPPK
5282 819.3723 1636.7300 1636.7119 11.1 1 1 1.9 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
6767 940.9276 1879.8406 1879.8258 7.90 0 1 2.3 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
363 414.1842 826.3538 826.3677 -16.8 0 1 0.81 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
415 414.1881 826.3616 826.3677 -7.33 0 1 0.89 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
706 441.1942 880.3738 880.3562 20.0 0 1 0.97 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
1083 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
4482 751.8416 1501.6686 1501.6658 1.90 1 1 2.1 +1Score > 17 indicates identity AEHRGGSGNFAEDR
348 414.1832 826.3518 826.3677 -19.2 0 1 0.89 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
766 441.2004 880.3862 880.3926 -7.26 0 1 1.2 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
2710 603.2741 1204.5336 1204.5295 3.46 0 1 1.7 +1Score > 16 indicates identity HACGYELAER
5612 846.3846 1690.7546 1690.7403 8.49 0 1 1.6 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
481 414.1881 826.3616 826.3677 -7.33 0 1 0.9 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
653 441.1916 880.3686 880.3562 14.1 0 1 1 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
1041 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
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