MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5801–5900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5609 846.3846 1690.7546
5610 846.3846 1690.7546
5611 846.3846 1690.7546
5613 846.3846 1690.7546
5615 846.3846 1690.7546
5617 846.3846 1690.7546
5618 846.3846 1690.7546
5619 846.3846 1690.7546
5621 846.3846 1690.7546
5623 846.3846 1690.7546
5625 846.3846 1690.7546
5626 846.3846 1690.7546
5627 846.3846 1690.7546
5628 846.3846 1690.7546
5629 846.3846 1690.7546
5630 846.3846 1690.7546
5631 846.3846 1690.7546
5632 846.3846 1690.7546
5633 846.3846 1690.7546
5634 846.3846 1690.7546
5635 846.3846 1690.7546
5636 846.3846 1690.7546
5637 846.3846 1690.7546
5638 846.3846 1690.7546
5639 846.3846 1690.7546
5640 846.3846 1690.7546
5641 846.3846 1690.7546
5642 846.3846 1690.7546
5643 846.3846 1690.7546
5644 846.3846 1690.7546
5645 846.3846 1690.7546
5646 846.3846 1690.7546
5647 846.3846 1690.7546
5648 846.3846 1690.7546
5650 846.3846 1690.7546
5651 846.3846 1690.7546
5652 846.3846 1690.7546
5653 846.3846 1690.7546
5654 846.3846 1690.7546
5655 846.3846 1690.7546
5656 846.3846 1690.7546
5657 846.3846 1690.7546
5658 846.3846 1690.7546
5659 846.3846 1690.7546
5660 846.3846 1690.7546
5661 846.3846 1690.7546
5662 846.3846 1690.7546
5664 846.3846 1690.7546
5665 846.3846 1690.7546
5666 846.3846 1690.7546
5667 846.3846 1690.7546
5668 846.3846 1690.7546
5669 846.3872 1690.7598
5670 846.3872 1690.7598
5671 846.3873 1690.7600
5672 846.3879 1690.7612
5673 846.3880 1690.7614
5674 846.3887 1690.7628
5675 846.3895 1690.7644
5676 846.3905 1690.7664
5677 846.3907 1690.7668
5678 846.3909 1690.7672
5679 846.3910 1690.7674
5680 846.3922 1690.7698
5681 846.3922 1690.7698
5682 846.3924 1690.7702
5683 846.3927 1690.7708
5684 846.3937 1690.7728
5686 846.4002 1690.7858
5687 846.4003 1690.7860
5688 859.8819 1717.7492
5689 859.8835 1717.7524
5690 859.8837 1717.7528
5691 859.8864 1717.7582
5692 859.8907 1717.7668
5693 859.8907 1717.7668
5694 859.8907 1717.7668
5695 859.8907 1717.7668
5696 859.8907 1717.7668
5697 859.8907 1717.7668
5698 859.8907 1717.7668
5699 859.8907 1717.7668
5700 859.8907 1717.7668
5701 859.8907 1717.7668
5702 859.8907 1717.7668
5703 859.8907 1717.7668
5704 859.8907 1717.7668
5705 859.8907 1717.7668
5706 859.8907 1717.7668
5707 859.8907 1717.7668
5708 859.8907 1717.7668
5709 859.8907 1717.7668
5710 859.8907 1717.7668
5711 859.8907 1717.7668
5712 859.8907 1717.7668
5713 859.8907 1717.7668
5714 859.8907 1717.7668
5715 859.8907 1717.7668
5716 859.8907 1717.7668
5717 859.8907 1717.7668
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