MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5701–5800 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5496 832.8785 1663.7424
5497 832.8785 1663.7424
5498 832.8785 1663.7424
5499 832.8785 1663.7424
5500 832.8785 1663.7424
5501 832.8785 1663.7424
5502 832.8785 1663.7424
5503 832.8785 1663.7424
5504 832.8785 1663.7424
5505 832.8785 1663.7424
5506 832.8785 1663.7424
5507 832.8785 1663.7424
5508 832.8785 1663.7424
5509 832.8785 1663.7424
5510 832.8785 1663.7424
5512 832.8785 1663.7424
5513 832.8785 1663.7424
5514 832.8785 1663.7424
5516 832.8785 1663.7424
5517 832.8785 1663.7424
5518 832.8785 1663.7424
5519 832.8785 1663.7424
5520 832.8785 1663.7424
5521 832.8785 1663.7424
5522 832.8785 1663.7424
5523 832.8785 1663.7424
5524 832.8785 1663.7424
5525 832.8785 1663.7424
5526 846.3787 1690.7428
5527 846.3813 1690.7480
5529 846.3846 1690.7546
5530 846.3846 1690.7546
5531 846.3846 1690.7546
5532 846.3846 1690.7546
5533 846.3846 1690.7546
5534 846.3846 1690.7546
5535 846.3846 1690.7546
5536 846.3846 1690.7546
5537 846.3846 1690.7546
5538 846.3846 1690.7546
5539 846.3846 1690.7546
5540 846.3846 1690.7546
5541 846.3846 1690.7546
5542 846.3846 1690.7546
5543 846.3846 1690.7546
5544 846.3846 1690.7546
5545 846.3846 1690.7546
5546 846.3846 1690.7546
5547 846.3846 1690.7546
5548 846.3846 1690.7546
5549 846.3846 1690.7546
5550 846.3846 1690.7546
5551 846.3846 1690.7546
5552 846.3846 1690.7546
5553 846.3846 1690.7546
5554 846.3846 1690.7546
5555 846.3846 1690.7546
5556 846.3846 1690.7546
5557 846.3846 1690.7546
5558 846.3846 1690.7546
5559 846.3846 1690.7546
5560 846.3846 1690.7546
5561 846.3846 1690.7546
5562 846.3846 1690.7546
5563 846.3846 1690.7546
5564 846.3846 1690.7546
5566 846.3846 1690.7546
5567 846.3846 1690.7546
5568 846.3846 1690.7546
5569 846.3846 1690.7546
5570 846.3846 1690.7546
5572 846.3846 1690.7546
5573 846.3846 1690.7546
5574 846.3846 1690.7546
5575 846.3846 1690.7546
5576 846.3846 1690.7546
5577 846.3846 1690.7546
5579 846.3846 1690.7546
5581 846.3846 1690.7546
5582 846.3846 1690.7546
5584 846.3846 1690.7546
5585 846.3846 1690.7546
5586 846.3846 1690.7546
5587 846.3846 1690.7546
5588 846.3846 1690.7546
5589 846.3846 1690.7546
5592 846.3846 1690.7546
5593 846.3846 1690.7546
5594 846.3846 1690.7546
5595 846.3846 1690.7546
5596 846.3846 1690.7546
5598 846.3846 1690.7546
5599 846.3846 1690.7546
5600 846.3846 1690.7546
5601 846.3846 1690.7546
5602 846.3846 1690.7546
5603 846.3846 1690.7546
5604 846.3846 1690.7546
5605 846.3846 1690.7546
5606 846.3846 1690.7546
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