MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5601–5700 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5391 832.8785 1663.7424
5392 832.8785 1663.7424
5393 832.8785 1663.7424
5394 832.8785 1663.7424
5395 832.8785 1663.7424
5396 832.8785 1663.7424
5397 832.8785 1663.7424
5398 832.8785 1663.7424
5399 832.8785 1663.7424
5400 832.8785 1663.7424
5402 832.8785 1663.7424
5403 832.8785 1663.7424
5404 832.8785 1663.7424
5405 832.8785 1663.7424
5406 832.8785 1663.7424
5407 832.8785 1663.7424
5408 832.8785 1663.7424
5409 832.8785 1663.7424
5410 832.8785 1663.7424
5411 832.8785 1663.7424
5412 832.8785 1663.7424
5413 832.8785 1663.7424
5414 832.8785 1663.7424
5415 832.8785 1663.7424
5416 832.8785 1663.7424
5418 832.8785 1663.7424
5419 832.8785 1663.7424
5420 832.8785 1663.7424
5421 832.8785 1663.7424
5422 832.8785 1663.7424
5423 832.8785 1663.7424
5424 832.8785 1663.7424
5425 832.8785 1663.7424
5426 832.8785 1663.7424
5427 832.8785 1663.7424
5428 832.8785 1663.7424
5429 832.8785 1663.7424
5430 832.8785 1663.7424
5431 832.8785 1663.7424
5432 832.8785 1663.7424
5433 832.8785 1663.7424
5434 832.8785 1663.7424
5435 832.8785 1663.7424
5436 832.8785 1663.7424
5437 832.8785 1663.7424
5438 832.8785 1663.7424
5439 832.8785 1663.7424
5440 832.8785 1663.7424
5442 832.8785 1663.7424
5443 832.8785 1663.7424
5445 832.8785 1663.7424
5446 832.8785 1663.7424
5447 832.8785 1663.7424
5448 832.8785 1663.7424
5450 832.8785 1663.7424
5451 832.8785 1663.7424
5452 832.8785 1663.7424
5453 832.8785 1663.7424
5454 832.8785 1663.7424
5455 832.8785 1663.7424
5456 832.8785 1663.7424
5457 832.8785 1663.7424
5458 832.8785 1663.7424
5459 832.8785 1663.7424
5460 832.8785 1663.7424
5461 832.8785 1663.7424
5462 832.8785 1663.7424
5463 832.8785 1663.7424
5464 832.8785 1663.7424
5465 832.8785 1663.7424
5466 832.8785 1663.7424
5467 832.8785 1663.7424
5468 832.8785 1663.7424
5469 832.8785 1663.7424
5470 832.8785 1663.7424
5471 832.8785 1663.7424
5472 832.8785 1663.7424
5473 832.8785 1663.7424
5474 832.8785 1663.7424
5475 832.8785 1663.7424
5476 832.8785 1663.7424
5477 832.8785 1663.7424
5478 832.8785 1663.7424
5479 832.8785 1663.7424
5480 832.8785 1663.7424
5481 832.8785 1663.7424
5482 832.8785 1663.7424
5483 832.8785 1663.7424
5484 832.8785 1663.7424
5485 832.8785 1663.7424
5486 832.8785 1663.7424
5487 832.8785 1663.7424
5488 832.8785 1663.7424
5489 832.8785 1663.7424
5490 832.8785 1663.7424
5491 832.8785 1663.7424
5492 832.8785 1663.7424
5493 832.8785 1663.7424
5494 832.8785 1663.7424
5495 832.8785 1663.7424
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