MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5501–5600 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5274 819.3723 1636.7300
5276 819.3723 1636.7300
5278 819.3723 1636.7300
5279 819.3723 1636.7300
5280 819.3723 1636.7300
5281 819.3723 1636.7300
5283 819.3723 1636.7300
5284 819.3723 1636.7300
5285 819.3723 1636.7300
5288 819.3723 1636.7300
5289 819.3723 1636.7300
5292 819.3723 1636.7300
5293 819.3723 1636.7300
5294 819.3723 1636.7300
5295 819.3723 1636.7300
5296 819.3723 1636.7300
5297 819.3723 1636.7300
5298 819.3723 1636.7300
5299 819.3723 1636.7300
5300 819.3723 1636.7300
5301 819.3723 1636.7300
5302 819.3723 1636.7300
5303 819.3723 1636.7300
5304 819.3723 1636.7300
5305 819.3723 1636.7300
5306 819.3723 1636.7300
5307 819.3723 1636.7300
5308 819.3723 1636.7300
5309 819.3723 1636.7300
5311 819.3723 1636.7300
5312 819.3723 1636.7300
5313 819.3723 1636.7300
5314 819.3723 1636.7300
5315 819.3723 1636.7300
5316 819.3723 1636.7300
5317 819.3723 1636.7300
5318 819.3723 1636.7300
5319 819.3723 1636.7300
5320 819.3723 1636.7300
5321 819.3723 1636.7300
5322 819.3723 1636.7300
5324 819.3723 1636.7300
5326 819.3723 1636.7300
5327 819.3723 1636.7300
5328 819.3723 1636.7300
5329 819.3723 1636.7300
5330 819.3723 1636.7300
5331 819.3723 1636.7300
5332 819.3723 1636.7300
5333 819.3723 1636.7300
5334 819.3723 1636.7300
5335 819.3723 1636.7300
5336 819.3723 1636.7300
5337 819.3723 1636.7300
5338 819.3723 1636.7300
5339 819.3723 1636.7300
5340 819.3723 1636.7300
5342 819.3723 1636.7300
5343 819.3723 1636.7300
5344 819.3723 1636.7300
5345 819.3723 1636.7300
5347 819.3723 1636.7300
5348 819.3723 1636.7300
5350 819.3723 1636.7300
5351 819.3723 1636.7300
5352 819.3723 1636.7300
5353 819.3723 1636.7300
5354 819.3723 1636.7300
5355 819.3723 1636.7300
5356 819.3723 1636.7300
5359 819.3821 1636.7496
5360 819.3845 1636.7544
5361 819.3871 1636.7596
5363 819.3881 1636.7616
5364 832.8785 1663.7424
5365 832.8785 1663.7424
5366 832.8785 1663.7424
5367 832.8785 1663.7424
5368 832.8785 1663.7424
5369 832.8785 1663.7424
5370 832.8785 1663.7424
5371 832.8785 1663.7424
5372 832.8785 1663.7424
5373 832.8785 1663.7424
5374 832.8785 1663.7424
5375 832.8785 1663.7424
5376 832.8785 1663.7424
5377 832.8785 1663.7424
5378 832.8785 1663.7424
5379 832.8785 1663.7424
5380 832.8785 1663.7424
5381 832.8785 1663.7424
5382 832.8785 1663.7424
5383 832.8785 1663.7424
5384 832.8785 1663.7424
5385 832.8785 1663.7424
5386 832.8785 1663.7424
5387 832.8785 1663.7424
5388 832.8785 1663.7424
5389 832.8785 1663.7424
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