MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 5401–5500 (out of 7290)

Page: Previous 1 …50 51 52 53 54 55 56 57 58 59 60 … 73 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5167 805.8662 1609.7178
5168 805.8662 1609.7178
5169 805.8662 1609.7178
5170 805.8662 1609.7178
5171 805.8662 1609.7178
5172 805.8662 1609.7178
5173 805.8662 1609.7178
5174 805.8662 1609.7178
5175 805.8662 1609.7178
5176 805.8662 1609.7178
5177 805.8662 1609.7178
5178 805.8662 1609.7178
5179 805.8662 1609.7178
5180 805.8662 1609.7178
5181 805.8662 1609.7178
5182 805.8662 1609.7178
5183 805.8662 1609.7178
5184 805.8662 1609.7178
5185 805.8662 1609.7178
5186 805.8662 1609.7178
5187 805.8662 1609.7178
5188 805.8662 1609.7178
5189 805.8662 1609.7178
5190 805.8662 1609.7178
5191 805.8662 1609.7178
5192 805.8662 1609.7178
5193 805.8662 1609.7178
5194 805.8662 1609.7178
5195 805.8662 1609.7178
5196 805.8662 1609.7178
5197 805.8662 1609.7178
5198 805.8662 1609.7178
5199 805.8662 1609.7178
5200 805.8662 1609.7178
5201 805.8662 1609.7178
5202 819.3723 1636.7300
5203 819.3723 1636.7300
5204 819.3723 1636.7300
5205 819.3723 1636.7300
5206 819.3723 1636.7300
5207 819.3723 1636.7300
5208 819.3723 1636.7300
5209 819.3723 1636.7300
5211 819.3723 1636.7300
5212 819.3723 1636.7300
5213 819.3723 1636.7300
5214 819.3723 1636.7300
5215 819.3723 1636.7300
5216 819.3723 1636.7300
5217 819.3723 1636.7300
5218 819.3723 1636.7300
5219 819.3723 1636.7300
5220 819.3723 1636.7300
5221 819.3723 1636.7300
5223 819.3723 1636.7300
5224 819.3723 1636.7300
5226 819.3723 1636.7300
5227 819.3723 1636.7300
5228 819.3723 1636.7300
5229 819.3723 1636.7300
5230 819.3723 1636.7300
5231 819.3723 1636.7300
5232 819.3723 1636.7300
5233 819.3723 1636.7300
5234 819.3723 1636.7300
5235 819.3723 1636.7300
5236 819.3723 1636.7300
5237 819.3723 1636.7300
5238 819.3723 1636.7300
5239 819.3723 1636.7300
5240 819.3723 1636.7300
5241 819.3723 1636.7300
5242 819.3723 1636.7300
5243 819.3723 1636.7300
5244 819.3723 1636.7300
5245 819.3723 1636.7300
5246 819.3723 1636.7300
5248 819.3723 1636.7300
5249 819.3723 1636.7300
5250 819.3723 1636.7300
5251 819.3723 1636.7300
5252 819.3723 1636.7300
5253 819.3723 1636.7300
5256 819.3723 1636.7300
5257 819.3723 1636.7300
5258 819.3723 1636.7300
5259 819.3723 1636.7300
5260 819.3723 1636.7300
5261 819.3723 1636.7300
5262 819.3723 1636.7300
5263 819.3723 1636.7300
5264 819.3723 1636.7300
5265 819.3723 1636.7300
5266 819.3723 1636.7300
5268 819.3723 1636.7300
5269 819.3723 1636.7300
5270 819.3723 1636.7300
5271 819.3723 1636.7300
5272 819.3723 1636.7300
5273 819.3723 1636.7300
Page: Previous 1 …50 51 52 53 54 55 56 57 58 59 60 … 73 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/