MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5101–5200 (out of 7290)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4846 778.8539 1555.6932
4848 778.8539 1555.6932
4849 778.8539 1555.6932
4850 778.8539 1555.6932
4852 778.8539 1555.6932
4853 778.8539 1555.6932
4855 778.8539 1555.6932
4856 778.8539 1555.6932
4857 778.8539 1555.6932
4860 778.8539 1555.6932
4861 778.8539 1555.6932
4862 778.8539 1555.6932
4863 778.8539 1555.6932
4864 778.8539 1555.6932
4865 778.8539 1555.6932
4866 778.8539 1555.6932
4867 778.8539 1555.6932
4868 778.8539 1555.6932
4869 778.8539 1555.6932
4870 778.8539 1555.6932
4871 778.8539 1555.6932
4872 778.8539 1555.6932
4873 778.8539 1555.6932
4874 778.8590 1555.7034
4875 778.8602 1555.7058
4876 778.8624 1555.7102
4877 778.8641 1555.7136
4878 792.3444 1582.6742
4879 792.3463 1582.6780
4880 792.3463 1582.6780
4881 792.3475 1582.6804
4882 792.3476 1582.6806
4883 792.3477 1582.6808
4884 792.3481 1582.6816
4885 792.3481 1582.6816
4886 792.3481 1582.6816
4887 792.3489 1582.6832
4888 792.3490 1582.6834
4889 792.3493 1582.6840
4890 792.3495 1582.6844
4891 792.3498 1582.6850
4892 792.3505 1582.6864
4893 792.3525 1582.6904
4894 792.3600 1582.7054
4895 792.3600 1582.7054
4896 792.3600 1582.7054
4897 792.3600 1582.7054
4898 792.3600 1582.7054
4899 792.3600 1582.7054
4900 792.3600 1582.7054
4901 792.3600 1582.7054
4902 792.3600 1582.7054
4903 792.3600 1582.7054
4904 792.3600 1582.7054
4905 792.3600 1582.7054
4906 792.3600 1582.7054
4907 792.3600 1582.7054
4908 792.3600 1582.7054
4909 792.3600 1582.7054
4910 792.3600 1582.7054
4911 792.3600 1582.7054
4912 792.3600 1582.7054
4913 792.3600 1582.7054
4914 792.3600 1582.7054
4915 792.3600 1582.7054
4916 792.3600 1582.7054
4917 792.3600 1582.7054
4918 792.3600 1582.7054
4920 792.3600 1582.7054
4921 792.3600 1582.7054
4922 792.3600 1582.7054
4923 792.3600 1582.7054
4924 792.3600 1582.7054
4925 792.3600 1582.7054
4926 792.3600 1582.7054
4927 792.3600 1582.7054
4928 792.3600 1582.7054
4929 792.3600 1582.7054
4930 792.3600 1582.7054
4931 792.3600 1582.7054
4932 792.3600 1582.7054
4933 792.3600 1582.7054
4934 792.3600 1582.7054
4935 792.3600 1582.7054
4936 792.3600 1582.7054
4937 792.3600 1582.7054
4938 792.3600 1582.7054
4939 792.3600 1582.7054
4941 792.3600 1582.7054
4943 792.3600 1582.7054
4944 792.3600 1582.7054
4945 792.3600 1582.7054
4946 792.3600 1582.7054
4947 792.3600 1582.7054
4948 792.3600 1582.7054
4949 792.3600 1582.7054
4950 792.3600 1582.7054
4952 792.3600 1582.7054
4953 792.3600 1582.7054
4954 792.3600 1582.7054
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