MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5001–5100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4733 778.8539 1555.6932
4734 778.8539 1555.6932
4735 778.8539 1555.6932
4736 778.8539 1555.6932
4737 778.8539 1555.6932
4738 778.8539 1555.6932
4739 778.8539 1555.6932
4740 778.8539 1555.6932
4741 778.8539 1555.6932
4743 778.8539 1555.6932
4744 778.8539 1555.6932
4745 778.8539 1555.6932
4746 778.8539 1555.6932
4747 778.8539 1555.6932
4748 778.8539 1555.6932
4749 778.8539 1555.6932
4750 778.8539 1555.6932
4751 778.8539 1555.6932
4752 778.8539 1555.6932
4753 778.8539 1555.6932
4754 778.8539 1555.6932
4755 778.8539 1555.6932
4756 778.8539 1555.6932
4757 778.8539 1555.6932
4758 778.8539 1555.6932
4759 778.8539 1555.6932
4760 778.8539 1555.6932
4761 778.8539 1555.6932
4762 778.8539 1555.6932
4763 778.8539 1555.6932
4765 778.8539 1555.6932
4766 778.8539 1555.6932
4767 778.8539 1555.6932
4768 778.8539 1555.6932
4769 778.8539 1555.6932
4770 778.8539 1555.6932
4772 778.8539 1555.6932
4773 778.8539 1555.6932
4774 778.8539 1555.6932
4775 778.8539 1555.6932
4776 778.8539 1555.6932
4777 778.8539 1555.6932
4778 778.8539 1555.6932
4780 778.8539 1555.6932
4781 778.8539 1555.6932
4782 778.8539 1555.6932
4783 778.8539 1555.6932
4784 778.8539 1555.6932
4786 778.8539 1555.6932
4787 778.8539 1555.6932
4788 778.8539 1555.6932
4789 778.8539 1555.6932
4790 778.8539 1555.6932
4791 778.8539 1555.6932
4792 778.8539 1555.6932
4793 778.8539 1555.6932
4794 778.8539 1555.6932
4795 778.8539 1555.6932
4796 778.8539 1555.6932
4797 778.8539 1555.6932
4798 778.8539 1555.6932
4800 778.8539 1555.6932
4801 778.8539 1555.6932
4802 778.8539 1555.6932
4803 778.8539 1555.6932
4804 778.8539 1555.6932
4805 778.8539 1555.6932
4806 778.8539 1555.6932
4807 778.8539 1555.6932
4808 778.8539 1555.6932
4809 778.8539 1555.6932
4810 778.8539 1555.6932
4811 778.8539 1555.6932
4812 778.8539 1555.6932
4813 778.8539 1555.6932
4815 778.8539 1555.6932
4816 778.8539 1555.6932
4817 778.8539 1555.6932
4818 778.8539 1555.6932
4820 778.8539 1555.6932
4822 778.8539 1555.6932
4823 778.8539 1555.6932
4824 778.8539 1555.6932
4825 778.8539 1555.6932
4826 778.8539 1555.6932
4827 778.8539 1555.6932
4828 778.8539 1555.6932
4829 778.8539 1555.6932
4830 778.8539 1555.6932
4831 778.8539 1555.6932
4832 778.8539 1555.6932
4833 778.8539 1555.6932
4835 778.8539 1555.6932
4837 778.8539 1555.6932
4838 778.8539 1555.6932
4839 778.8539 1555.6932
4840 778.8539 1555.6932
4841 778.8539 1555.6932
4842 778.8539 1555.6932
4845 778.8539 1555.6932
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