MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4901–5000 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4622 765.3478 1528.6810
4623 765.3478 1528.6810
4624 765.3478 1528.6810
4625 765.3478 1528.6810
4626 765.3478 1528.6810
4627 765.3478 1528.6810
4628 765.3478 1528.6810
4630 765.3478 1528.6810
4631 765.3478 1528.6810
4633 765.3478 1528.6810
4634 765.3478 1528.6810
4637 765.3478 1528.6810
4638 765.3478 1528.6810
4639 765.3478 1528.6810
4640 765.3478 1528.6810
4642 765.3478 1528.6810
4643 765.3478 1528.6810
4644 765.3478 1528.6810
4645 765.3478 1528.6810
4646 765.3478 1528.6810
4647 765.3478 1528.6810
4648 765.3478 1528.6810
4649 765.3478 1528.6810
4650 765.3478 1528.6810
4651 765.3478 1528.6810
4652 765.3478 1528.6810
4653 765.3478 1528.6810
4654 765.3478 1528.6810
4655 765.3478 1528.6810
4656 765.3478 1528.6810
4657 765.3478 1528.6810
4658 765.3478 1528.6810
4659 765.3478 1528.6810
4660 765.3478 1528.6810
4661 765.3478 1528.6810
4662 765.3478 1528.6810
4663 765.3478 1528.6810
4664 765.3478 1528.6810
4665 765.3478 1528.6810
4666 765.3478 1528.6810
4668 765.3478 1528.6810
4669 765.3478 1528.6810
4670 765.3478 1528.6810
4671 765.3478 1528.6810
4672 765.3478 1528.6810
4673 765.3478 1528.6810
4674 765.3478 1528.6810
4676 765.3478 1528.6810
4678 765.3478 1528.6810
4679 765.3478 1528.6810
4680 765.3478 1528.6810
4682 765.3478 1528.6810
4683 765.3478 1528.6810
4684 765.3478 1528.6810
4685 765.3478 1528.6810
4688 765.3478 1528.6810
4689 765.3478 1528.6810
4690 765.3478 1528.6810
4691 765.3478 1528.6810
4692 765.3478 1528.6810
4693 765.3478 1528.6810
4694 765.3478 1528.6810
4695 765.3478 1528.6810
4696 765.3478 1528.6810
4697 765.3478 1528.6810
4698 765.3478 1528.6810
4699 765.3478 1528.6810
4700 765.3478 1528.6810
4701 765.3478 1528.6810
4702 765.3478 1528.6810
4703 765.3478 1528.6810
4704 765.3478 1528.6810
4705 765.3478 1528.6810
4706 765.3478 1528.6810
4707 765.3478 1528.6810
4708 765.3516 1528.6886
4709 765.3526 1528.6906
4710 765.3530 1528.6914
4711 765.3534 1528.6922
4712 765.3539 1528.6932
4713 765.3550 1528.6954
4714 765.3551 1528.6956
4715 765.3593 1528.7040
4716 778.8539 1555.6932
4717 778.8539 1555.6932
4718 778.8539 1555.6932
4719 778.8539 1555.6932
4720 778.8539 1555.6932
4721 778.8539 1555.6932
4722 778.8539 1555.6932
4723 778.8539 1555.6932
4724 778.8539 1555.6932
4725 778.8539 1555.6932
4726 778.8539 1555.6932
4727 778.8539 1555.6932
4728 778.8539 1555.6932
4729 778.8539 1555.6932
4730 778.8539 1555.6932
4731 778.8539 1555.6932
4732 778.8539 1555.6932
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