MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2421 576.2618 1150.5090 1150.4933 13.7 0 2 1.1 +1Score > 15 indicates identity CMTQMNIPR + Deamidated (NQ)
6907 954.4337 1906.8528 1906.8690 -8.48 1 2 1.7 +1Score > 17 indicates identity LSAEDNARVNVAMNQTR + 3 Deamidated (NQ); Oxidation (M)
1918 535.7434 1069.4722 1069.4862 -13.1 0 2 0.58 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
4442 751.8416 1501.6686 1501.6909 -14.8 1 2 1.6 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
6883 954.4337 1906.8528 1906.8784 -13.4 0 2 1.7 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
806 441.2004 880.3862 880.3821 4.72 1 2 0.92 +1Score > 15 indicates identity NRSSTCR + Deamidated (NQ)
4489 751.8416 1501.6686 1501.6727 -2.71 1 2 1.6 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
6266 900.4092 1798.8038 1798.7904 7.46 0 2 1 +1Score > 15 indicates identity SHQHTTQTSGQGMLER + 2 Deamidated (NQ)
2717 603.2741 1204.5336 1204.5473 -11.3 0 2 1.3 +1Score > 16 indicates identity YDVHNLDSSR
4204 724.8293 1447.6440 1447.6725 -19.7 1 2 0.73 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
5905 873.3969 1744.7792 1744.7839 -2.64 1 2 2 +1Score > 18 indicates identity GGQYCSADYQAQLKR + Deamidated (NQ)
2031 549.2496 1096.4846 1096.4753 8.49 1 2 0.99 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
1260 481.7188 961.4230 961.4352 -12.7 0 2 0.94 +1Score > 15 indicates identity NVQANGTEK + 2 Deamidated (NQ)
6070 886.9030 1771.7914 1771.7725 10.7 1 2 1.3 +1Score > 16 indicates identity QEVCAMMALNMLRR + 2 Deamidated (NQ); 3 Oxidation (M)
672 441.1928 880.3710 880.3562 16.8 0 2 0.71 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
5899 873.3969 1744.7792 1744.7648 8.31 1 2 2.1 +1Score > 18 indicates identity EEEKAFLMECASTGK + Oxidation (M)
6568 927.4214 1852.8282 1852.8625 -18.5 1 2 1.5 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
4986 792.3600 1582.7054 1582.6875 11.4 0 2 0.98 +1Score > 15 indicates identity VYFCDPQSPWQR + Deamidated (NQ)
6410 913.9153 1825.8160 1825.8087 4.02 1 2 1.7 +1Score > 17 indicates identity NGMECGIGVKNYNDVR + Deamidated (NQ)
983 468.2127 934.4108 934.4178 -7.46 0 2 1.1 +1Score > 15 indicates identity MNTTPSQR + Deamidated (NQ)
1270 481.7188 961.4230 961.4253 -2.38 0 2 0.95 +1Score > 15 indicates identity SEFEHSAR
1711 522.2372 1042.4598 1042.4576 2.17 0 2 0.59 +1Score > 13 indicates identity GFADAIMMR + 2 Oxidation (M)
6995 967.9399 1933.8652 1933.8840 -9.68 1 2 1.6 +1Score > 17 indicates identity MGWSGEPTTSELKNPER + Oxidation (M)
3808 697.8171 1393.6196 1393.6031 11.9 0 2 1.4 +1Score > 16 indicates identity TEQVNQQLMER + 3 Deamidated (NQ); Oxidation (M)
3769 697.8171 1393.6196 1393.6361 -11.8 0 2 1.4 +1Score > 16 indicates identity LEQDLTDAGFER + Deamidated (NQ)
5649 846.3846 1690.7546 1690.7403 8.49 0 2 1.3 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
5911 873.3969 1744.7792 1744.7648 8.31 1 2 2.1 +1Score > 18 indicates identity EEEKAFLMECASTGK + Oxidation (M)
6309 900.4092 1798.8038 1798.7870 9.35 1 2 1 +1Score > 15 indicates identity QPNGQVWSPQNDDRR + 3 Deamidated (NQ)
469 414.1881 826.3616 826.3569 5.74 0 2 0.69 +1Score > 13 indicates identity ANAQQHR + 3 Deamidated (NQ)
1211 481.7188 961.4230 961.4253 -2.38 0 2 0.96 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
2035 549.2496 1096.4846 1096.4753 8.49 1 2 1 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
6572 927.4214 1852.8282 1852.8625 -18.5 1 2 1.5 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
4443 751.8416 1501.6686 1501.6909 -14.8 1 2 1.6 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
6896 954.4337 1906.8528 1906.8784 -13.4 0 2 1.7 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
412 414.1881 826.3616 826.3677 -7.33 0 2 0.7 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
6487 913.9153 1825.8160 1825.7900 14.2 0 2 1.7 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
5685 846.3956 1690.7766 1690.7984 -12.9 0 2 1.8 +1Score > 17 indicates identity IEQVSTWQGMNLER + Deamidated (NQ)
5275 819.3723 1636.7300 1636.7119 11.1 1 2 1.5 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
5597 846.3846 1690.7546 1690.7661 -6.78 1 2 1.3 +1Score > 16 indicates identity GYSDTQGMLGVKYSW
6416 913.9153 1825.8160 1825.8482 -17.6 1 2 1.7 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
2190 562.7557 1123.4968 1123.4894 6.65 0 2 0.95 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
3625 684.3109 1366.6072 1366.6299 -16.6 1 2 1.3 +1Score > 16 indicates identity GAAMEGQYKGAQR + Deamidated (NQ)
2308 576.2618 1150.5090 1150.4891 17.4 0 2 1.1 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
659 441.1922 880.3698 880.3562 15.4 0 2 0.8 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
3630 684.3109 1366.6072 1366.6339 -19.5 1 2 1.4 +1Score > 16 indicates identity NFGMPAPEGYRK + Deamidated (NQ)
5935 873.3969 1744.7792 1744.8103 -17.8 0 2 2.2 +1Score > 18 indicates identity YAHLNANWLEMNNR
6543 927.4214 1852.8282 1852.8389 -5.74 0 2 1.6 +1Score > 17 indicates identity SLPWQAVWEMYCQR
3984 711.3232 1420.6318 1420.6512 -13.6 1 2 1.5 +1Score > 16 indicates identity QMMGMEPKQIAR + 2 Deamidated (NQ)
2100 549.2496 1096.4846 1096.4753 8.49 1 2 1.1 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
1633 522.2302 1042.4458 1042.4277 17.4 0 2 0.63 +1Score > 13 indicates identity FSLGNMDDK + Deamidated (NQ); Oxidation (M)
2769 603.2839 1204.5532 1204.5684 -12.6 0 2 2.6 +1Score > 19 indicates identity SAETLSGGEAQR
4844 778.8539 1555.6932 1555.7163 -14.8 0 2 1.2 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
6335 900.4163 1798.8180 1798.8416 -13.1 1 2 1.6 +1Score > 16 indicates identity RYTDMPMLVMLEER + Oxidation (M)
6404 913.9153 1825.8160 1825.8206 -2.49 1 2 1.8 +1Score > 17 indicates identity DVNGQFRGGPAWYMAR + 2 Deamidated (NQ)
6859 954.4337 1906.8528 1906.8811 -14.8 0 2 1.8 +1Score > 17 indicates identity SPVGSLFYDFFGPNTMK + Deamidated (NQ)
6299 900.4092 1798.8038 1798.7870 9.35 0 2 1.1 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
1934 535.7434 1069.4722 1069.4862 -13.1 1 2 0.64 +1Score > 13 indicates identity EAMGFSEKR + Oxidation (M)
689 441.1935 880.3724 880.3562 18.4 0 2 0.8 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
1632 522.2287 1042.4428 1042.4277 14.5 0 2 0.67 +1Score > 13 indicates identity FSLGNMDDK + Deamidated (NQ); Oxidation (M)
663 441.1925 880.3704 880.3596 12.3 0 2 0.84 +1Score > 14 indicates identity LNSQMNR + 3 Deamidated (NQ); Oxidation (M)
5809 859.8907 1717.7668 1717.7876 -12.1 1 2 1.2 +1Score > 15 indicates identity SMQLLAEDRTDHMR + Oxidation (M)
5916 873.3969 1744.7792 1744.7648 8.31 1 2 2.2 +1Score > 18 indicates identity EEEKAFLMECASTGK + Oxidation (M)
1823 535.7434 1069.4722 1069.4862 -13.1 0 2 0.64 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1843 535.7434 1069.4722 1069.4862 -13.1 0 2 0.65 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
5987 873.3969 1744.7792 1744.7735 3.28 0 2 2.3 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
6998 967.9399 1933.8652 1933.9017 -18.9 1 2 1.7 +1Score > 17 indicates identity QDIDRWQSTSVNDVLR + 3 Deamidated (NQ)
2484 589.7679 1177.5212 1177.5363 -12.8 1 2 0.94 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
2036 549.2496 1096.4846 1096.4753 8.49 1 2 1.1 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
5325 819.3723 1636.7300 1636.7119 11.1 1 2 1.6 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
444 414.1881 826.3616 826.3677 -7.33 0 2 0.75 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1074 468.2127 934.4108 934.4178 -7.46 1 2 1.2 +1Score > 15 indicates identity LDEDCKR
4433 751.8416 1501.6686 1501.6943 -17.1 1 2 1.8 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
4843 778.8539 1555.6932 1555.7114 -11.7 0 2 1.2 +1Score > 15 indicates identity SDATADQHQLQALR + 3 Deamidated (NQ)
411 414.1881 826.3616 826.3677 -7.33 0 2 0.75 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
675 441.1929 880.3712 880.3709 0.45 1 2 0.78 +1Score > 13 indicates identity NDMSNKR + Deamidated (NQ); Oxidation (M)
1031 468.2127 934.4108 934.4178 -7.46 1 2 1.2 +1Score > 15 indicates identity LDEDCKR
5580 846.3846 1690.7546 1690.7767 -13.0 1 2 1.4 +1Score > 16 indicates identity MDSNNQIEPCLSRK
6993 967.9399 1933.8652 1933.9017 -18.9 1 2 1.7 +1Score > 17 indicates identity QDIDRWQSTSVNDVLR + 3 Deamidated (NQ)
2145 562.7557 1123.4968 1123.4894 6.65 0 2 1 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
6737 940.9276 1879.8406 1879.8305 5.41 1 2 2 +1Score > 17 indicates identity QSHGNPLMKQGHQMQR + 4 Deamidated (NQ)
6892 954.4337 1906.8528 1906.8784 -13.4 0 2 1.9 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
1107 468.2127 934.4108 934.4290 -19.5 1 2 1.3 +1Score > 15 indicates identity LNRSCER + Deamidated (NQ)
1306 495.2180 988.4214 988.4324 -11.1 0 2 0.66 +1Score > 13 indicates identity MDYEFLR + Oxidation (M)
5933 873.3969 1744.7792 1744.7735 3.28 0 2 2.3 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
1283 481.7188 961.4230 961.4352 -12.7 0 2 1.1 +1Score > 15 indicates identity VNDQEISR + 2 Deamidated (NQ)
4632 765.3478 1528.6810 1528.6868 -3.74 0 2 1.1 +1Score > 15 indicates identity FFAAGADLNEMAEK + Oxidation (M)
2027 549.2496 1096.4846 1096.4753 8.49 1 2 1.1 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
3626 684.3109 1366.6072 1366.6299 -16.6 1 2 1.5 +1Score > 16 indicates identity GAAMEGQYKGAQR + Deamidated (NQ)
142 387.1814 772.3482 772.3351 17.0 0 2 0.67 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
532 427.6943 853.3740 853.3600 16.5 0 2 0.67 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
1046 468.2127 934.4108 934.4178 -7.46 0 2 1.3 +1Score > 15 indicates identity MNTTPSQR + Deamidated (NQ)
2742 603.2741 1204.5336 1204.5394 -4.75 0 2 1.5 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
128 387.1810 772.3474 772.3351 15.9 0 2 0.68 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
6259 900.4092 1798.8038 1798.8308 -15.0 0 2 1.2 +1Score > 15 indicates identity IYDSDSILSMHHEPR
2749 603.2741 1204.5336 1204.5506 -14.1 0 2 1.5 +1Score > 16 indicates identity GCGITVDQAER
701 441.1940 880.3734 880.3562 19.5 0 2 0.85 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4113 724.8293 1447.6440 1447.6725 -19.7 1 2 0.85 +1Score > 13 indicates identity ECAAEITDKAGQR
6894 954.4337 1906.8528 1906.8784 -13.4 0 2 2 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
3483 670.8048 1339.5950 1339.6143 -14.4 0 2 1.6 +1Score > 16 indicates identity TGVSNTLENEFK + 2 Deamidated (NQ)
2246 562.7557 1123.4968 1123.4968 0.046 0 2 1.1 +1Score > 14 indicates identity HADTAMYTAK + Oxidation (M)
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