MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4801–4900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4521 751.8416 1501.6686
4523 751.8416 1501.6686
4524 751.8416 1501.6686
4525 751.8416 1501.6686
4526 751.8416 1501.6686
4527 751.8416 1501.6686
4528 751.8416 1501.6686
4529 751.8416 1501.6686
4530 751.8416 1501.6686
4531 751.8416 1501.6686
4532 751.8416 1501.6686
4533 751.8416 1501.6686
4534 751.8416 1501.6686
4535 751.8416 1501.6686
4536 751.8416 1501.6686
4537 751.8416 1501.6686
4538 751.8416 1501.6686
4539 751.8416 1501.6686
4540 751.8416 1501.6686
4541 751.8416 1501.6686
4542 751.8416 1501.6686
4543 751.8416 1501.6686
4544 751.8416 1501.6686
4545 751.8416 1501.6686
4546 751.8416 1501.6686
4547 751.8416 1501.6686
4548 751.8416 1501.6686
4549 751.8416 1501.6686
4550 751.8416 1501.6686
4551 751.8416 1501.6686
4552 751.8416 1501.6686
4553 751.8464 1501.6782
4554 765.3339 1528.6532
4555 765.3391 1528.6636
4556 765.3422 1528.6698
4557 765.3478 1528.6810
4558 765.3478 1528.6810
4559 765.3478 1528.6810
4560 765.3478 1528.6810
4561 765.3478 1528.6810
4562 765.3478 1528.6810
4563 765.3478 1528.6810
4564 765.3478 1528.6810
4565 765.3478 1528.6810
4566 765.3478 1528.6810
4567 765.3478 1528.6810
4568 765.3478 1528.6810
4569 765.3478 1528.6810
4570 765.3478 1528.6810
4571 765.3478 1528.6810
4572 765.3478 1528.6810
4573 765.3478 1528.6810
4574 765.3478 1528.6810
4575 765.3478 1528.6810
4576 765.3478 1528.6810
4577 765.3478 1528.6810
4578 765.3478 1528.6810
4579 765.3478 1528.6810
4580 765.3478 1528.6810
4581 765.3478 1528.6810
4582 765.3478 1528.6810
4583 765.3478 1528.6810
4584 765.3478 1528.6810
4585 765.3478 1528.6810
4586 765.3478 1528.6810
4587 765.3478 1528.6810
4588 765.3478 1528.6810
4589 765.3478 1528.6810
4590 765.3478 1528.6810
4591 765.3478 1528.6810
4592 765.3478 1528.6810
4593 765.3478 1528.6810
4594 765.3478 1528.6810
4595 765.3478 1528.6810
4596 765.3478 1528.6810
4597 765.3478 1528.6810
4598 765.3478 1528.6810
4599 765.3478 1528.6810
4600 765.3478 1528.6810
4601 765.3478 1528.6810
4602 765.3478 1528.6810
4603 765.3478 1528.6810
4604 765.3478 1528.6810
4605 765.3478 1528.6810
4606 765.3478 1528.6810
4607 765.3478 1528.6810
4608 765.3478 1528.6810
4609 765.3478 1528.6810
4610 765.3478 1528.6810
4611 765.3478 1528.6810
4612 765.3478 1528.6810
4613 765.3478 1528.6810
4614 765.3478 1528.6810
4615 765.3478 1528.6810
4616 765.3478 1528.6810
4617 765.3478 1528.6810
4618 765.3478 1528.6810
4619 765.3478 1528.6810
4620 765.3478 1528.6810
4621 765.3478 1528.6810
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