MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4701–4800 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4387 738.3355 1474.6564
4388 738.3355 1474.6564
4389 738.3355 1474.6564
4390 738.3365 1474.6584
4392 751.8416 1501.6686
4393 751.8416 1501.6686
4394 751.8416 1501.6686
4395 751.8416 1501.6686
4396 751.8416 1501.6686
4397 751.8416 1501.6686
4398 751.8416 1501.6686
4399 751.8416 1501.6686
4400 751.8416 1501.6686
4401 751.8416 1501.6686
4402 751.8416 1501.6686
4403 751.8416 1501.6686
4404 751.8416 1501.6686
4405 751.8416 1501.6686
4407 751.8416 1501.6686
4408 751.8416 1501.6686
4409 751.8416 1501.6686
4410 751.8416 1501.6686
4411 751.8416 1501.6686
4412 751.8416 1501.6686
4413 751.8416 1501.6686
4414 751.8416 1501.6686
4416 751.8416 1501.6686
4419 751.8416 1501.6686
4420 751.8416 1501.6686
4421 751.8416 1501.6686
4424 751.8416 1501.6686
4425 751.8416 1501.6686
4426 751.8416 1501.6686
4427 751.8416 1501.6686
4428 751.8416 1501.6686
4429 751.8416 1501.6686
4430 751.8416 1501.6686
4431 751.8416 1501.6686
4439 751.8416 1501.6686
4446 751.8416 1501.6686
4447 751.8416 1501.6686
4448 751.8416 1501.6686
4449 751.8416 1501.6686
4450 751.8416 1501.6686
4453 751.8416 1501.6686
4454 751.8416 1501.6686
4456 751.8416 1501.6686
4457 751.8416 1501.6686
4458 751.8416 1501.6686
4460 751.8416 1501.6686
4462 751.8416 1501.6686
4463 751.8416 1501.6686
4464 751.8416 1501.6686
4465 751.8416 1501.6686
4466 751.8416 1501.6686
4467 751.8416 1501.6686
4468 751.8416 1501.6686
4469 751.8416 1501.6686
4470 751.8416 1501.6686
4471 751.8416 1501.6686
4472 751.8416 1501.6686
4473 751.8416 1501.6686
4474 751.8416 1501.6686
4475 751.8416 1501.6686
4478 751.8416 1501.6686
4479 751.8416 1501.6686
4480 751.8416 1501.6686
4481 751.8416 1501.6686
4483 751.8416 1501.6686
4484 751.8416 1501.6686
4485 751.8416 1501.6686
4486 751.8416 1501.6686
4487 751.8416 1501.6686
4488 751.8416 1501.6686
4490 751.8416 1501.6686
4491 751.8416 1501.6686
4492 751.8416 1501.6686
4493 751.8416 1501.6686
4495 751.8416 1501.6686
4496 751.8416 1501.6686
4497 751.8416 1501.6686
4498 751.8416 1501.6686
4499 751.8416 1501.6686
4501 751.8416 1501.6686
4503 751.8416 1501.6686
4505 751.8416 1501.6686
4506 751.8416 1501.6686
4507 751.8416 1501.6686
4508 751.8416 1501.6686
4509 751.8416 1501.6686
4510 751.8416 1501.6686
4511 751.8416 1501.6686
4512 751.8416 1501.6686
4513 751.8416 1501.6686
4514 751.8416 1501.6686
4515 751.8416 1501.6686
4516 751.8416 1501.6686
4518 751.8416 1501.6686
4519 751.8416 1501.6686
4520 751.8416 1501.6686
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