MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4501–4600 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4162 724.8293 1447.6440
4163 724.8293 1447.6440
4164 724.8293 1447.6440
4165 724.8293 1447.6440
4166 724.8293 1447.6440
4167 724.8293 1447.6440
4170 724.8293 1447.6440
4171 724.8293 1447.6440
4172 724.8293 1447.6440
4173 724.8293 1447.6440
4175 724.8293 1447.6440
4176 724.8293 1447.6440
4178 724.8293 1447.6440
4179 724.8293 1447.6440
4180 724.8293 1447.6440
4181 724.8293 1447.6440
4182 724.8293 1447.6440
4183 724.8293 1447.6440
4184 724.8293 1447.6440
4186 724.8293 1447.6440
4188 724.8293 1447.6440
4190 724.8293 1447.6440
4192 724.8293 1447.6440
4193 724.8293 1447.6440
4194 724.8293 1447.6440
4195 724.8293 1447.6440
4196 724.8293 1447.6440
4197 724.8293 1447.6440
4198 724.8293 1447.6440
4199 724.8293 1447.6440
4200 724.8293 1447.6440
4201 724.8293 1447.6440
4202 724.8293 1447.6440
4203 724.8293 1447.6440
4205 724.8293 1447.6440
4206 724.8293 1447.6440
4207 724.8293 1447.6440
4208 724.8293 1447.6440
4210 724.8293 1447.6440
4211 724.8293 1447.6440
4212 724.8293 1447.6440
4213 724.8293 1447.6440
4214 724.8293 1447.6440
4215 724.8293 1447.6440
4216 724.8293 1447.6440
4217 724.8293 1447.6440
4219 724.8293 1447.6440
4220 724.8293 1447.6440
4221 724.8293 1447.6440
4222 724.8293 1447.6440
4223 724.8293 1447.6440
4224 724.8293 1447.6440
4225 724.8293 1447.6440
4226 724.8293 1447.6440
4227 724.8293 1447.6440
4228 724.8293 1447.6440
4229 724.8430 1447.6714
4230 738.3220 1474.6294
4231 738.3355 1474.6564
4232 738.3355 1474.6564
4233 738.3355 1474.6564
4234 738.3355 1474.6564
4235 738.3355 1474.6564
4236 738.3355 1474.6564
4237 738.3355 1474.6564
4238 738.3355 1474.6564
4239 738.3355 1474.6564
4240 738.3355 1474.6564
4241 738.3355 1474.6564
4242 738.3355 1474.6564
4243 738.3355 1474.6564
4244 738.3355 1474.6564
4245 738.3355 1474.6564
4246 738.3355 1474.6564
4247 738.3355 1474.6564
4248 738.3355 1474.6564
4249 738.3355 1474.6564
4250 738.3355 1474.6564
4251 738.3355 1474.6564
4252 738.3355 1474.6564
4253 738.3355 1474.6564
4254 738.3355 1474.6564
4255 738.3355 1474.6564
4256 738.3355 1474.6564
4257 738.3355 1474.6564
4258 738.3355 1474.6564
4259 738.3355 1474.6564
4261 738.3355 1474.6564
4262 738.3355 1474.6564
4263 738.3355 1474.6564
4264 738.3355 1474.6564
4265 738.3355 1474.6564
4267 738.3355 1474.6564
4268 738.3355 1474.6564
4269 738.3355 1474.6564
4270 738.3355 1474.6564
4271 738.3355 1474.6564
4272 738.3355 1474.6564
4273 738.3355 1474.6564
4274 738.3355 1474.6564
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