MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4037 711.3232 1420.6318
4038 711.3232 1420.6318
4039 711.3232 1420.6318
4040 711.3232 1420.6318
4041 711.3232 1420.6318
4042 711.3232 1420.6318
4043 711.3232 1420.6318
4044 711.3232 1420.6318
4045 711.3232 1420.6318
4046 711.3232 1420.6318
4047 711.3232 1420.6318
4048 711.3232 1420.6318
4049 711.3232 1420.6318
4050 711.3232 1420.6318
4051 711.3232 1420.6318
4052 711.3232 1420.6318
4053 711.3232 1420.6318
4055 711.3232 1420.6318
4056 711.3232 1420.6318
4057 711.3232 1420.6318
4058 711.3232 1420.6318
4059 711.3232 1420.6318
4060 711.3232 1420.6318
4061 711.3232 1420.6318
4062 711.3232 1420.6318
4063 711.3232 1420.6318
4064 711.3232 1420.6318
4065 711.3232 1420.6318
4066 711.3307 1420.6468
4068 724.8293 1447.6440
4069 724.8293 1447.6440
4070 724.8293 1447.6440
4071 724.8293 1447.6440
4072 724.8293 1447.6440
4073 724.8293 1447.6440
4074 724.8293 1447.6440
4075 724.8293 1447.6440
4076 724.8293 1447.6440
4077 724.8293 1447.6440
4079 724.8293 1447.6440
4080 724.8293 1447.6440
4081 724.8293 1447.6440
4082 724.8293 1447.6440
4083 724.8293 1447.6440
4084 724.8293 1447.6440
4085 724.8293 1447.6440
4086 724.8293 1447.6440
4087 724.8293 1447.6440
4088 724.8293 1447.6440
4091 724.8293 1447.6440
4092 724.8293 1447.6440
4093 724.8293 1447.6440
4096 724.8293 1447.6440
4098 724.8293 1447.6440
4101 724.8293 1447.6440
4103 724.8293 1447.6440
4105 724.8293 1447.6440
4110 724.8293 1447.6440
4114 724.8293 1447.6440
4115 724.8293 1447.6440
4116 724.8293 1447.6440
4117 724.8293 1447.6440
4118 724.8293 1447.6440
4119 724.8293 1447.6440
4120 724.8293 1447.6440
4123 724.8293 1447.6440
4124 724.8293 1447.6440
4127 724.8293 1447.6440
4128 724.8293 1447.6440
4129 724.8293 1447.6440
4131 724.8293 1447.6440
4132 724.8293 1447.6440
4133 724.8293 1447.6440
4134 724.8293 1447.6440
4135 724.8293 1447.6440
4136 724.8293 1447.6440
4137 724.8293 1447.6440
4138 724.8293 1447.6440
4139 724.8293 1447.6440
4140 724.8293 1447.6440
4141 724.8293 1447.6440
4142 724.8293 1447.6440
4143 724.8293 1447.6440
4144 724.8293 1447.6440
4145 724.8293 1447.6440
4146 724.8293 1447.6440
4147 724.8293 1447.6440
4149 724.8293 1447.6440
4150 724.8293 1447.6440
4151 724.8293 1447.6440
4152 724.8293 1447.6440
4153 724.8293 1447.6440
4154 724.8293 1447.6440
4155 724.8293 1447.6440
4156 724.8293 1447.6440
4157 724.8293 1447.6440
4158 724.8293 1447.6440
4159 724.8293 1447.6440
4160 724.8293 1447.6440
4161 724.8293 1447.6440
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